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Biosynthesis of Biopolyester from the mixed substrates of glycerin and fatty acid/amino acids

Research Project

Project/Area Number 24550179
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental chemistry
Research InstitutionRyukoku University

Principal Investigator

NAKAOKI Takahiko  龍谷大学, 理工学部, 教授 (90257824)

Co-Investigator(Kenkyū-buntansha) ISHII Daisuke  東京大学, 大学院農学生命科学研究科, 特任助教 (70415074)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Keywordsポリ(3-ヒドロキシアルカノエート) / 生合成 / グリセリン / 脂肪酸 / アミノ酸 / 収量 / バイオプラスチック / ポリヒドロキシアルカノエート / ブロック共重合体 / 力学物性 / バイオディーゼル燃料
Outline of Final Research Achievements

Plant oils are composed of triglycerides. Glycerin is derived from plant oil, however glycerin is not widely used in industrial applications. As for poly(3-hydroxyalkanoate) (P3HA) biosynthesis from glycerin, there have been only a small number of studies reported because glycerin is not a good carbon source for PHA biosynthesis. When the mixed substrate of glycerin and fatty acid is used as carbon sources for biosynthesizing P3HA by Ralstonia eutropha, we found that the addition of small amount of fatty acid to glycerin makes the polymer yield enhance. The polymer yield is about 1.3 times larger than the use of original carbon sources. Amino acids are another good carbon source to accumulate P3HA. In this case, phosphate-free medium was used because amino acid itself contains nitrogen. The cultivation of leucine and glycerin as carbon sources successfully improved the polymer yield with 3 times larger polymer yield than the original ones.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (9 results)

All 2015 2014 2013 2012

All Journal Article (3 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results) Presentation (6 results)

  • [Journal Article] T. SHirono, T. Nakaoki2015

    • Author(s)
      Biosynthesis of Poly(3-hydroxyalkanoate) by Ralstonia Eutropha Culyivated in a Glycerol/amino acids-containing Medium
    • Journal Title

      J. Polym Environ

      Volume: 23

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biosynthesis of Poly(3-hydroxyalkanoate) from Amino Acids in Medium with Nitrogen, Phosphate, and Magnesium, or some Combination of these Nutrientsels2014

    • Author(s)
      M. Sakamoto, Y. Kimura, D. Ishii, and T. Nakaoki
    • Journal Title

      J. Polym Environ

      Volume: 22 Pages: 488-493

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Production of Poly(3-hydroxyalkanoate)s by Pseudomonas putida Cultivated in a Glycerol/Nonanoic Acid-Containing Medium2013

    • Author(s)
      T. Miura, D. Ishii, and T. Nakaoki
    • Journal Title

      J. Polym. Environ

      Volume: 21 Pages: 760-765

    • Related Report
      2013 Research-status Report
  • [Presentation] Relationship between Mechanical Properties and Molecular Strictures of P(3HBV-block-3HB) Copolymer Biosynthesized by Wautersia eutropha2014

    • Author(s)
      Junya Yasui, Risa Yamagishi and Takahiko Nakaoki
    • Organizer
      The 10th SPSJ International Polymer Conference
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2014-12-02 – 2014-12-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] W. eutrophaを用いて生合成したP(3HBV-block-3HB)ブロック共重合体の引張特性2014

    • Author(s)
      安居 潤哉・山岸 理沙・中沖 隆彦
    • Organizer
      第63回高分子討論会
    • Place of Presentation
      長崎大学文教キャンパス(長崎県長崎市)
    • Year and Date
      2014-09-24 – 2014-09-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] W. eutrophaを用いたポリ(ヒドロキシブチレート-b-ヒドロキシバリレート)ブロック共重合体における力学物性の検討2014

    • Author(s)
      安居 潤哉・山岸 理沙・中沖 隆彦
    • Organizer
      繊維学会年次大会
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Year and Date
      2014-06-11 – 2014-06-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] W. eutrophaを用いたポリ(ヒドロキシブチレート-b-ヒドロキシバリレート)ブロック共重合体の生合成と力学物性2014

    • Author(s)
      安居 潤哉・山岸 理沙・中沖 隆彦
    • Organizer
      高分子学会年次大会
    • Place of Presentation
      名古屋国際会議場(愛知県名古屋市)
    • Year and Date
      2014-05-28 – 2014-05-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] P. putidaによるバイオディーゼル燃料の副生グリセリンを炭素源としたポリ(3-ヒドロキシアルカノエート)の生合成2013

    • Author(s)
      中沖隆彦、高橋輝行、安居潤哉
    • Organizer
      高分子討論会
    • Place of Presentation
      金沢大学
    • Related Report
      2013 Research-status Report
  • [Presentation] バイオディーゼル燃料合成時に副生するグリセリンからのポリヒドロキシアルカノエートの生合成2012

    • Author(s)
      佐篠慶・野出明奈・石井大輔・中沖隆彦
    • Organizer
      高分子討論会
    • Place of Presentation
      名古屋工業大学
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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