Studies of the low-cost immobilization system for recombinant proteins using beta-GRP and curdlan.
| Project/Area Number |
24560954
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Health Sciences University of Hokkaido (2013-2014)
Hokkaido University (2012) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
落合 正則 北海道大学, 低温科学研究所, 准教授 (10241382)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | タンパク質 / バイオテクノロジー / バイオリアクター / 生物・生体工学 / ナノ材料 |
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| Outline of Final Research Achievements |
Amino terminal domain of Silkworm βGRP (GRP-tag) tightly and specifically associates with a water-insoluble β-1,3-glucan, curdlan. We have studied a novel immobilization system for recombinant proteins based on the association between the GRP-tag and curdlan. The recombinant proteins fused to the GRP-tag were expressed in several hosts and were immobilized in one step on the curdlan beads from cell lysates and medium. Furthermore, we succeeded in preparation of a curdlan disk and curdlan sheet as a support carrier for GRP-tag.
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Report
(4 results)
Research Products
(6 results)
