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Studies of the low-cost immobilization system for recombinant proteins using beta-GRP and curdlan.

Research Project

Project/Area Number 24560954
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionHealth Sciences University of Hokkaido (2013-2014)
Hokkaido University (2012)

Principal Investigator

HORIUCHI Masataka  北海道医療大学, 薬学部, 講師 (90322825)

Co-Investigator(Kenkyū-buntansha) 落合 正則  北海道大学, 低温科学研究所, 准教授 (10241382)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywordsタンパク質 / バイオテクノロジー / バイオリアクター / 生物・生体工学 / ナノ材料
Outline of Final Research Achievements

Amino terminal domain of Silkworm βGRP (GRP-tag) tightly and specifically associates with a water-insoluble β-1,3-glucan, curdlan. We have studied a novel immobilization system for recombinant proteins based on the association between the GRP-tag and curdlan. The recombinant proteins fused to the GRP-tag were expressed in several hosts and were immobilized in one step on the curdlan beads from cell lysates and medium. Furthermore, we succeeded in preparation of a curdlan disk and curdlan sheet as a support carrier for GRP-tag.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (6 results)

All 2014 2013 2012

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (2 results)

  • [Journal Article] The N-terminal domain of TIR domain-containing adaptor molecule-1, TICAM-1.2014

    • Author(s)
      Kumeta H, Sakakibara H, Enokizono Y, Ogura K, Horiuchi M, Matsumoto M, Seya T, Inagaki F.
    • Journal Title

      J Biomol NMR

      Volume: 58(3) Issue: 3 Pages: 227-30

    • DOI

      10.1007/s10858-014-9819-1

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation2013

    • Author(s)
      K. Takemoto (1), T. Matsuda (2)., 他12名
    • Journal Title

      Sci. Rep

      Volume: 3巻 Issue: 1 Pages: 2629-2629

    • DOI

      10.1038/srep02629

    • NAID

      120006995014

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structures and interface mapping of the TIR domain-containing adaptor molecules involved in interferon signaling.2013

    • Author(s)
      Enokizono Y, Kumeta H, Funami K, Horiuchi M, Sarmiento J, Yamashita K, Standley DM, Matsumoto M, Seya T, Inagaki F.
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 110(49) Issue: 49 Pages: 19908-19913

    • DOI

      10.1073/pnas.1222811110

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A low-cost affinity purification system using beta-1,3-glucan recognition protein and curdlan beads2012

    • Author(s)
      Horiuchi, M., Takahasi, K., Kobashigawa, Y., Ochiai, M. and Inagaki, F.
    • Journal Title

      PEDS

      Volume: 25 Issue: 8 Pages: 405-413

    • DOI

      10.1093/protein/gzs028

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] オッセオインテグレーション獲得に関わるタンパク質の昆虫細胞を用いた生産2014

    • Author(s)
      堀内留美,堀内正隆,横山敦郎
    • Organizer
      平成26年度 日本補綴歯科学会 東北北海道支部学術大会
    • Place of Presentation
      市民交流プラザ (郡山市)
    • Year and Date
      2014-10-25 – 2014-10-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] Investigation of the inhibitory factors for osseointegration in the early stage of the implantation.2014

    • Author(s)
      Horiuchi M, Horiuchi R, Ochiai M, Yokoyama A
    • Organizer
      The 28th Annual Symposium of the Protein Society
    • Place of Presentation
      Manchester Grand Hyatt (San Diego, USA)
    • Year and Date
      2014-07-27 – 2014-07-30
    • Related Report
      2014 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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