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Development of highly sensitive biomolecular interaction detection system utilizing affinity peptide conjugated cushion protein as the anchor domain

Research Project

Project/Area Number 24560963
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionOkayama University

Principal Investigator

IMANAKA Hiroyuki  岡山大学, 自然科学研究科, 助教 (10379711)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordsバイオ分子 / 固定化 / 相互作用 / 超好熱菌 / タンパク質 / ペプチド / 親和性 / 耐熱性 / T7ファージ
Outline of Final Research Achievements

Biomolecular immobilization is one of the basic technologies for microanalysis, diagnosis and proteome analysis. We have tried to establish the highly sensitive biomolecular interaction detection system on solid substrate by utilizing techniques of precise control of biomolecular immobilization. As the results, high interaction detection sensitivity was achieved through the combination of selected cushion protein and solid surface affinity peptide for immobilization of target biomolecule.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (13 results)

All 2014 2013 Other

All Presentation (13 results) (of which Invited: 1 results)

  • [Presentation] Influence of Conformational Constraint and Spatial Arrangement of Ligand Peptide on the Detection of Peptide-Protein Interaction2014

    • Author(s)
      Hiroyuki Imanaka, Miki Ishimaru, Takashi Fujii, Naoyuki Ishida and Koreyoshi Imamura
    • Organizer
      Young Asian Biochemical Engineers' Community 2014
    • Place of Presentation
      Chia-yi, Taiwan
    • Year and Date
      2014-11-06 – 2014-11-08
    • Related Report
      2014 Annual Research Report
  • [Presentation] Design of highly sensitive peptide-protein interaction detection system adopting CS complex formation as the model2014

    • Author(s)
      Runa Matsushita , Naoyuki Ishida , Koreyoshi Imamura and Hiroyuki Imanaka
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Screening of a novel gold affinity peptide and its application on protein immobilization2014

    • Author(s)
      Yojiro Shigemori, Kaori Yoshida, Koreyoshi Imamura, YuichiroTakahashi and Hiroyuki Imanaka
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] ペプチド-タンパク質間相互作用を制御する因子の検討2014

    • Author(s)
      松下瑠奈,石田尚之,今村維克,今中洋行
    • Organizer
      第46回化学工学会秋季大会
    • Place of Presentation
      福岡
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] バイオ分子と固体材料をつなぐ2014

    • Author(s)
      今中洋行
    • Organizer
      農芸化学会中四国支部第17回若手シンポジウム
    • Place of Presentation
      岡山
    • Year and Date
      2014-05-16 – 2014-05-17
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 金表面親和性ペプチドを用いた機能的タンパク質固定化2013

    • Author(s)
      重森陽士郎・今村維克・高橋裕一郎・今中洋行
    • Organizer
      日本農芸化学会中四国支部第36回講演会
    • Place of Presentation
      島根
    • Related Report
      2013 Research-status Report
  • [Presentation] Utilization of hydrophilic polystyrene affinity peptide for functional biomolecular immobilization and effective site specific peptide binder screening

    • Author(s)
      H. Imanaka, R. Matsushita, T. Takimoto, T. Kunikata, K. Imamura, and K. Nakanishi
    • Organizer
      23rd American Peptide Symposium
    • Place of Presentation
      ハワイ(アメリカ合衆国)
    • Related Report
      2013 Research-status Report
  • [Presentation] 固体材料親和性ペプチドタグを用いた機能的バイオ分子固定化技術の開発とその応用

    • Author(s)
      今中 洋行・重森 陽士郎・松下 瑠奈・ 瀧本 貴之・今村 維克
    • Organizer
      化学工学会 第45回秋季大会
    • Place of Presentation
      岡山
    • Related Report
      2013 Research-status Report
  • [Presentation] 新規金表面親和性ペプチドの同定及びタンパク質固定化への応用

    • Author(s)
      重森陽士郎・石田尚之・今村維克・高橋裕一郎・今中洋行
    • Organizer
      第65回日本生物工学会大会
    • Place of Presentation
      広島
    • Related Report
      2013 Research-status Report
  • [Presentation] ペプチド-タンパク質間相互作用検出に及ぼすリガンドペプチド束縛の影響

    • Author(s)
      今中洋行・石丸実季・藤井崇史・石田尚之・今村維克
    • Organizer
      化学工学会 第79回年会
    • Place of Presentation
      岐阜
    • Related Report
      2013 Research-status Report
  • [Presentation] 新規金表面親和性ペプチドタグの開発

    • Author(s)
      高橋拓也,清水友樹,今村維克,今中洋行
    • Organizer
      日本生物工学会西日本支部 第2回講演会
    • Place of Presentation
      岡山
    • Related Report
      2012 Research-status Report
  • [Presentation] 重森 陽士郎,吉田 香織,石田 尚之,今村 維克,今中 洋行,高橋 裕一郎

    • Author(s)
      新規金表面親和性ペプチドのスクリーニングおよび結合特性解析評価
    • Organizer
      化学工学会 第78年会
    • Place of Presentation
      大阪
    • Related Report
      2012 Research-status Report
  • [Presentation] 松下 瑠奈,石田 尚之,今村 維克,今中 洋行

    • Author(s)
      CS複合体形成をモデルとしたバイオ分子間相互作用に及ぼすリガンド固定化の影響
    • Organizer
      化学工学会 第78年会
    • Place of Presentation
      大阪
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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