Screening of bioactive peptide from marine metagenome and specific amplification methods for potential genes for drug discovery

• Project/Area Number: 24560964
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Hiroshima University
• Principal Investigator: OKAMURA YOSHIKO 広島大学, 先端物質科学研究科, 准教授 (80405513)
• Co-Investigator(Kenkyū-buntansha): SAITO Yumiko 広島大学, 大学院総合科学研究科, 教授 (00215568)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: メタゲノム / GPCR / 創薬ターゲット / ペプチド合成酵素遺伝子 / ペプチドリガンドスクリーニング / 海洋メタゲノム / リガンドスクリーニング

Outline of Final Research Achievements

The methods for drug screening using genomic resources from VBNC (viable but non-culturable) bacteria were developed. The surrogate ligand of orphan GPCR was screened among metagenomic library and we successfully found the candidate and its gene cluster. This result suggests that the gene resources from VBNC bacteria would be applicable to drug discvery. Moreover, we established the method that the whole genome harboring target genes for drug discovery were amplified specifically among the majority of non-target.

Research Products

• [Journal Article] バイオミディア 小さな生き物の大きな仕事 (2013)
  • Author(s): 岡村好子
  • Journal Title: 生物工学会誌 Volume: 91 Pages: 155-155
  • Peer Reviewed
• [Journal Article] Characterization of ciliary targeting sequence of rat melanin-concentrating hormone receptor 1 (2013)
  • Author(s): Nagata A, Hamamoto A, Horikawa M, Yoshimura K, Takeda S, Saito Y
  • Journal Title: General and Comparative Endocrinology Volume: 188 Issue: 1 Pages: 159
  • DOI: 10.1016/j.ygcen.2013.02.020
  • Peer Reviewed
• [Journal Article] Mutation of Phe318 within the NPxxY(x)5,6F motif in melanin-concentrating hormone receptor 1 results in an efficient signaling activity. (2012)
  • Author(s): Hamamoto A, Horikawa M, Saho T, Saito Y.
  • Journal Title: Front. Endocrinology Volume: 3.147 Pages: 1-10
  • DOI: 10.3389/fendo.2012.00147
  • Peer Reviewed
• [Presentation] メタゲノムアプローチを用いた好中球活性化ペプチド生合成遺伝子の同定 (2015)
  • Author(s): 高橋宏和, 椎田敦之, 鈴木 克彦, 竹山春子,岡村好子
  • Organizer: 第17回マリンバイオテクノロジー学会大会
  • Place of Presentation: 東京海洋大学、東京
  • Year and Date: 2015-05-30 – 2015-05-31
• [Presentation] ボンベシン様ペプチド受容体BRS-3のアゴニスト探索－マリンメタゲノムライブラリーの適用－ (2014)
  • Author(s): 徳丸雄一、岡村好子, 斎藤祐見子
  • Organizer: 第11回GPCR研究会
  • Place of Presentation: 日本科学未来館 みらいCANホール、東京
  • Year and Date: 2014-05-09 – 2014-05-10
• [Presentation] Utilization of seaweed biomass for functional lipid production. (2014)
  • Author(s): Kim Arafiles, Yuri Eramoto, Hiroaki Iwasaka, Yoshiko Okamura, Yukihiko Matsumura, Yutaka Nakashimada, Tsunehiro Aki
  • Organizer: 105th American Oil Chemists' Society Annual Meeting & Expo
  • Place of Presentation: Henry B. Gonzalez Convention Center, San Antonio, Texas, USA
  • Year and Date: 2014-05-04 – 2014-05-07
• [Presentation] 非リボソーム合成酵素遺伝子を有する細菌特異的なゲノム増幅法の開発 (2013)
  • Author(s): 岡村好子、高橋宏和
  • Organizer: 第15回マリンバイオテクノロジー学会大会
  • Place of Presentation: 沖縄県市町村自治会館
• [Presentation] カイメン共在バクテリアメタゲノムライブラリーを用いた抗炎症ペプチドのin sillicoスクリーニング (2013)
  • Author(s): 椎田敦之、鈴木克彦、竹山春子、岡村好子
  • Organizer: 第15回マリンバイオテクノロジー学会大会
  • Place of Presentation: 沖縄県市町村自治会館
• [Presentation] カイメン共在バクテリアメタゲノムを用いた好中球調節ペプチドの高効率スクリーニング (2013)
  • Author(s): 椎田敦之、高橋宏和、鈴木克彦、竹山春子、岡村好子
  • Organizer: 第６５回生物工学会大会
  • Place of Presentation: 広島国際会議場
• [Presentation] Screening of a Bioactive Compounds and Their Genes from a Metagenomic Library of Sponge- Associated Bacteria (2013)
  • Author(s): Yoshiko Okamura
  • Organizer: BIT's 3rd Annual World Congress of Marine Biotechnology
  • Place of Presentation: Hangzou Blossom Water Museum Hotel, Hangzou, China
  • Invited
• [Presentation] オーファン受容体bombesin receptor subtype-3 (BRS-3)の代替リガンド探索 (2013)
  • Author(s): 徳丸雄一、岡村好子, 斎藤祐見子
  • Organizer: 日本動物学会第84回岡山大会2013
  • Place of Presentation: 岡山大学津山キャンパス
• [Presentation] 特異的プライマーを用いた環状DNAの増幅 (2013)
  • Author(s): 岡村好子
  • Organizer: 第７回日本ゲノム微生物学会年会
  • Place of Presentation: 長浜バイオ大学
• [Presentation] 非リボソーム合成酵素遺伝子を有する細菌特異的なゲノム増幅法の開発 (2013)
  • Author(s): 岡村好子、高橋宏和
  • Organizer: 第１５回マリンバイオテクノロジー学会
  • Place of Presentation: 沖縄県市町村自治会館
• [Presentation] Screening of a Bioactive Compounds and Their Genes from a Metagenomic Library of Sponge-Associated Bacteria (2013)
  • Author(s): Yoshiko Okamura
  • Organizer: BIT's 3rd Annual World Congress of Marine Biotechnology
  • Place of Presentation: Hangzou Blossom Water Museum Hotel, Hangzou, China
  • Invited
• [Presentation] Study of Specific Gene Amplification with Rolling Cycle Amplification (2012)
  • Author(s): Masahiro Suemitsu, Hirokazu Takahashi, Yoshiko Okamura
  • Organizer: APMBC2012 (Asia-Pacific Marine Biotechnology Conference)
  • Place of Presentation: CUL-PORT, Kochi, Japan
• [Presentation] GPCRを介した情報伝達－古典的概念から新しい概念へ－
  • Author(s): 斎藤祐見子
  • Organizer: 日本神経化学会公開シンポジウム（兼オーガナイザー）“構造生物学から創薬まで：GPCR研究がもたらすパラダイムシフト”
  • Place of Presentation: 神戸
  • Invited
• [Presentation] “Structure-function insight on the MCH receptor” MSH and MCH: Evolution from classical body change
  • Author(s): Yumiko Saito
  • Organizer: 26th Conference of European Comparative Endocrinologists
  • Place of Presentation: Zurich, Switzerland
  • Invited
• [Presentation] オーファン受容体BRS-3の代替リガンド及びBRS-3.5の内在性リガンド探索
  • Author(s): 徳丸雄一、益田恵子、岩越栄子、浮穴和義、岡村好子、斎藤祐見子
  • Organizer: ２０１３年度 日本動物学会中国四国支部 広島県例会
  • Place of Presentation: 広島大学大学院理学研究科
• [Book] 実践治療薬 (2012)
  • Author(s): 斎藤祐見子
  • Total Pages: 376
  • Publisher: 金芳堂
• [Patent(Industrial Property Rights)] 標的DNAの特異的増幅法 (2012)
  • Inventor(s): 岡村好子
  • Industrial Property Rights Holder: 広島大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2012-261404
  • Filing Date: 2012-11-29