Understanding the molecular functions of the condensin complex in chromosome condensation

• Project/Area Number: 24570006
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: The University of Tokyo
• Principal Investigator: SUTANI Takashi 東京大学, 分子細胞生物学研究所, 助教 (30401524)
• Research Collaborator: SAKATA Toyonori
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 染色体 / 染色体凝縮 / 染色体分配 / 転写 / 分裂酵母 / ChIP-seq法 / 染色体高次構造 / ChIP-seq / 単鎖DNA / 細胞周期

Outline of Final Research Achievements

The condensin plays an important role in shaping genomic DNA into compact, rod-like “condensed” chromosomes, and is required for faithful inheritance of genetic material. In this study, I aimed to reveal the molecular function of condensin in condensation through high resolution mapping of condensin binding locations in condensed chromosomes. I found that condensin targets and rewinds unwound DNA structure created by transcription during mitosis, thereby contributing to chromosome condensation.

Research Products

• [Journal Article] Dissecting the first and the second meiotic divisions using a marker-less drug-hypersensitive fission yeast. (2014)
  • Author(s): Yuki Aoi, Masamitsu Sato, Takashi Sutani, Katsuhiko Shirahige, Tarun M Kapoor, and Shigehiro A Kawashima.
  • Journal Title: Cell cycle Volume: 13 Issue: 8 Pages: 1327-1334
  • DOI: 10.4161/cc.28294
  • Peer Reviewed
• [Presentation] Condensin precludes interference from transcription-driven DNA unwinding in mitotic chromosome condensation (2014)
  • Author(s): Takashi Sutani
  • Organizer: The 9th 3R Symposium "3R and beyond"
  • Place of Presentation: 御殿場高原ホテル（静岡県御殿場市）
  • Year and Date: 2014-11-17 – 2014-11-21
• [Presentation] 染色体凝縮因子による染色体リセット (2014)
  • Author(s): 須谷尚史
  • Organizer: 国立遺伝学研究所研究会「表現型と遺伝子型のミッシングリンクをつなぐ」
  • Place of Presentation: 遺伝学研究所（静岡県三島市）
  • Year and Date: 2014-06-27 – 2014-06-28
  • Invited
• [Presentation] Expulsion of ongoing RNA polymerase II from mitotic chromosomes by condensin (2014)
  • Author(s): Takashi Sutani, Toyonori Sakata, Katsuhiko Shirahige
  • Organizer: 高等研究所カンファレンス "Chromoatin decoding"
  • Place of Presentation: 国際高等研究所（京都府木津川市）
  • Year and Date: 2014-05-12 – 2014-05-15
• [Presentation] Condensin complex negates an inhibitory effect of gene transcription on mitotic chromosome segregation. (2013)
  • Author(s): Takashi Sutani, Katsuhiko Shirahige
  • Organizer: 7th International Fission Yeast Meeting
  • Place of Presentation: University of London, London, UK
• [Presentation] Transcription Regulation by SMC proteins (2013)
  • Author(s): Takashi Sutani, Katsuhiko Shirahige
  • Organizer: Message from yeast to epigenetics
  • Place of Presentation: グランディア芳泉（福井県あわら市）
  • Invited
• [Presentation] コンデンシン複合体はM期遺伝子転写のもつ染色体分配に対する障害作用を解消する (2013)
  • Author(s): 須谷尚史、坂田豊典、中戸隆一郎、白髭克彦
  • Organizer: 国立遺伝学研究所研究会「染色体DNAの安定維持の分子メカニズム」
  • Place of Presentation: 国立遺伝学研究所（静岡県、三島市）
  • Invited
• [Presentation] Cohesin resolves interference between mitotic gene transcription and chromosome segregation. (2013)
  • Author(s): Takashi Sutani, Toyonori Sakata, Mai Ishibashi, Ryuichiro Nakato, Masashige Bando, Katsuhiko Shirahige
  • Organizer: 18th ICBM Symposium; SMC proteins from molecule to disease
  • Place of Presentation: 東京大学弥生講堂（東京都文京区）
• [Presentation] コンデンシンは遺伝子転写がもつM期染色体分配に対する阻害的効果を中和する (2012)
  • Author(s): 須谷尚史、中戸隆一郎、鈴木穣、白髭克彦
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場（福岡）
  • Invited
• [Presentation] コンデンシンは遺伝子転写がもつM期染色体分配に対する阻害的効果を中和する (2012)
  • Author(s): 須谷尚史、石橋舞、中戸隆一郎、白髭克彦
  • Organizer: 第30回染色体ワークショップ
  • Place of Presentation: 淡路夢舞台国際会議場（兵庫）