Investigation of communications in male and female gametophytic cells of land plants

• Project/Area Number: 24570045
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nagoya University
• Principal Investigator: KANAOKA Masahiro 名古屋大学, 理学(系)研究科(研究院), 助教 (10467277)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 花粉管ガイダンス / 花粉管 / 受精 / 胚珠

Outline of Final Research Achievements

(1) LURE1 althologue gene from T. concolor was isolated. pollen tube guidance assay using chimeric TfLURE1 and TcLURE1 identified important region for attraction in T. fournieri.
(2) A novel pollen tube attraction molecule was successfully identified by using a novel microfluidic pollen tube guidance system.
(3) a live imaging method for sperm attraction in Marchantia polymorpha was developed.

Research Products

• [Journal Article] Apoplastic ROS production upon pollination by RbohH and RbohJ in Arabidopsis (2015)
  • Author(s): 1)Kaya H, Iwano M, Takeda S, Kanaoka MM, Kimura S, Higashiyama T, Abe M, Kuchitsu K.
  • Journal Title: Plant Signaling Behavior Volume: 0 Issue: 2 Pages: 0-0
  • DOI: 10.4161/15592324.2014.989050
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Ca2+-Activated Reactive Oxygen Species Production by Arabidopsis RbohH and RbohJ Is Essential for Proper Pollen Tube Tip Growth (2014)
  • Author(s): 3)Kaya H, Nakajima R, Iwano M, Kanaoka MM, Kimura S, Takeda S, Kawarazaki T, Senzaki E, Hamamura Y, Higashiyama T, Takayama S, Abe M, Kuchitsu K.
  • Journal Title: Plant Cell Volume: 26 Pages: 1069-1080
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Pollen-pistil interactions in reproductive interference: comparisons of heterospecific pollen tube growth from alien species between two native Taraxacum species (2013)
  • Author(s): Nishida S., M. M. Kanaoka, Y. Hashimoto, K-I. Takakura, T. Nishida
  • Journal Title: Functional Ecology Volume: 28 Issue: 2 Pages: 450-457
  • DOI: 10.1111/1365-2435.12165
  • Peer Reviewed / Open Access
• [Journal Article] A microfluidic device for quantitative analysis of chemoattraction in plants (2013)
  • Author(s): 1. Mitsuhiro Horade, Masahiro M Kanaoka, Motoki Kuzuya, Tetsuya Higashiyama, Noritada Kaji
  • Journal Title: RSC Advances Volume: 3 Issue: 44 Pages: 22301-22307
  • DOI: 10.1039/c3ra42804d
  • Peer Reviewed
• [Journal Article] Arabidopsis homeodomain-leucine zipper IV proteins promote stomatal development and their ectopic expression induces stomata beyond the epidermis (2013)
  • Author(s): Kylee M Peterson, Christine Shyu, Christian A Burr, Robin J Horst, Masahiro M Kanaoka, Minami Omae, Yutaka Sato, and Keiko U Torii
  • Journal Title: Development Volume: 140 Issue: 9 Pages: 1936-1945
  • DOI: 10.1242/dev.090209
  • Peer Reviewed
• [Journal Article] Acquisition of LURE-Binding Activity at the Pollen Tube Tip of Torenia fournieri (2013)
  • Author(s): Satohiro Okuda, 他5名
  • Journal Title: Molecular Plant Volume: (印刷中)(in press) Issue: 4 Pages: 1074-1090
  • DOI: 10.1093/mp/sst050
  • Peer Reviewed
• [Presentation] Dynamics of stomatal behaviors and transcriptome in relation to environmental changes in Cardamine allotetraploid species (2014)
  • Author(s): Kanaoka, M.M
  • Organizer: Kyoto–Zurich Plant Workshop
  • Place of Presentation: 京都大学
  • Year and Date: 2014-12-22
  • Invited
• [Presentation] Sporophytic and gametophytic control of pollen tube guidance by secreted molecules (2014)
  • Author(s): Kanaoka MM
  • Organizer: 38th Naito Conference
  • Place of Presentation: シャトレーゼ札幌
  • Year and Date: 2014-10-08
• [Presentation] dentification of a novel pollen tube attractant in Torenia fournieri (2014)
  • Author(s): Kanaoka MM
  • Organizer: International ERATO Higashiyama Live-Holonics Symposium 2014
  • Place of Presentation: 名古屋大学
  • Year and Date: 2014-09-09
  • Invited
• [Presentation] Pollen Tube-in-a-Chip: A microfluidic devise for quantitative analysis of pollen tube guidance (2014)
  • Author(s): Kanaoka M, Horade M, Kuzuya M, Higashiyama T, Kaji N
  • Organizer: 23rd International Conference on Sexual Plant Reproduction, Porto, Portugal
  • Place of Presentation: Porto, Portugal
  • Year and Date: 2014-07-14
• [Presentation] Regulation of directional pollen tube growth by secreted molecules
  • Author(s): Masahiro Kanaoka, Motoki Kuzuya, Kei Hattori, Tetsuya Higashiyama
  • Organizer: 第55回日本植物生理学会大会
  • Place of Presentation: 富山大学
  • Invited
• [Presentation] 外来タンポポはなぜ強い?-在来種への繁殖干渉メカニズム-
  • Author(s): 西田佐知子、金岡 雅浩
  • Organizer: 第61回日本生態学会大会
  • Place of Presentation: 広島大学
• [Presentation] 異質倍数体植物の環境応答にみられる表現型可塑性の解析
  • Author(s): 青木優里、清水健太郎、東山哲也、清水(稲継)理恵、秋山玲子、金岡雅浩
  • Organizer: 日本植物学会第77回大会
  • Place of Presentation: 北海道大学
• [Presentation] トレニア花粉管先端における誘引物質LUREsの受容能獲得
  • Author(s): 奥田哲弘、鈴木孝征、森仁志、金岡雅浩、佐々木成江、東山哲也
  • Organizer: 日本植物学会第77回大会
  • Place of Presentation: 北海道大学
• [Presentation] Investigation of pollen tube guidance molecules and their roles for species-specificity in Torenia.
  • Author(s): Masahiro Kanaoka
  • Organizer: 77th Cold Spring Harbor Laboratory Symposium "The Biology of Plants"
  • Place of Presentation: Cold Spring Harbor Laboratory, USA
• [Presentation] トレニア成熟雌しべに見出された新規花粉管ガイダンス現象の解析
  • Author(s): 金岡雅浩
  • Organizer: 日本植物形態学会
  • Place of Presentation: 兵庫県立大学（兵庫県）
• [Presentation] トレニア胚珠に由来する新規花粉管ガイダンス現象とその因子の探索
  • Author(s): 葛谷元規
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 兵庫県立大学（兵庫県）
• [Presentation] 異質倍数化植物に見られる環境応答可塑性の解析
  • Author(s): 青木優里
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学（岡山県）
• [Book] 授業でそのまま使える最新高校生物解説書（岡田清孝、町田泰則 編） (2014)
  • Author(s): 金岡雅浩
  • Total Pages: 112
  • Publisher: 講談社サイエンティフィック
• [Book] Protoplasts from plant female gametophytes (in: Atlas of Plant Cell Structure) (Editors: Tetsuko Noguchi, Shigeyuki Kawano, Hirokazu Tsukaya, Sachihiro Matsunaga, Atsushi Sakai, Ichirou Karahara, Yasuko Hayashi ) (2014)
  • Author(s): Kanaoka, MM
  • Total Pages: 202
  • Publisher: Springer
• [Book] Pollen tube guidance toward the ovule (in: Atlas of Plant Cell Structure) (Editors: Tetsuko Noguchi, Shigeyuki Kawano, Hirokazu Tsukaya, Sachihiro Matsunaga, Atsushi Sakai, Ichirou Karahara, Yasuko Hayashi ) (2014)
  • Author(s): Kanaoka, MM
  • Total Pages: 202
  • Publisher: Springer
• [Book] 高校生物解説書 授業でそのまま使えるPowerPoint付き! 植物編 (2014)
  • Author(s): 町田 泰則, 岡田 清孝, 山本 興太朗 (監修) （分担執筆）
  • Total Pages: 112
  • Publisher: 講談社サイエンティフィク