Histological analysis and identification of the gene responsible for a germ cell deficient mutant in medaka

• Project/Area Number: 24570066
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Utsunomiya University
• Principal Investigator: MASARU Matsuda 宇都宮大学, バイオサイエンス教育研究センター, 教授 (20414013)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: メダカ / 性分化 / 生殖細胞 / 生殖腺

Outline of Final Research Achievements

A germ cell deficient mutant has no germ cells at 30 days after hatching stage. So, I analyzed when the germ cells were missing and I narrowed the chromosomal region located on the responsible gene. First, I searched recombinant progeny of the heterozygous mutant and the HNI strain, and found a few recombinants. Therefore, I mated heterozygous mutant with the Hd-rR strain and found 34 recombinants from 975 progeny. Since these recombinants were heterozygous, I performed mating experiment to obtain homozygous offsprings. Analyses of phenotypes of these homozygous offsprings narrowed the responsible region to 87 kb.

Research Products

• [Journal Article] Developmental and morphological studies in Japanese medaka with ultra-high resolution optical coherence tomography (2015)
  • Author(s): Gladys, F.M., Matsuda, M., Lim, Y., Jackin, B.J., Imai, T., Otani, Y., Yatagai, T., Cense, B.
  • Journal Title: Biomedical Optics Express Volume: 6 Issue: 2 Pages: 297-308
  • DOI: 10.1364/boe.6.000297
  • Peer Reviewed / Open Access
• [Journal Article] Spontaneous germline excision of Tol1, a DNA-based transposable element naturally occurring in the medaka fish genome (2014)
  • Author(s): Watanabe, K., Koga, H., Nakamura, K., Fujita, A., Hattori, A., Matsuda, M., Koga, A.
  • Journal Title: Genome Volume: 57 Issue: 4 Pages: 193-199
  • DOI: 10.1139/gen-2014-0011
  • Peer Reviewed
• [Journal Article] TALEN-mediated mutagenesis in zebrafish reveals a role for r-spondin 2 in fin ray and vertebral development (2014)
  • Author(s): Tatsumi, Y., Takeda, M., Matsuda, M., Suzuki, T., Yokoi, H.
  • Journal Title: FEBS Lett Volume: 558 Issue: 24 Pages: 4543-4550
  • DOI: 10.1016/j.febslet.2014.10.015
  • Peer Reviewed / Open Access
• [Journal Article] Co-option of Sox3 as the male-determining factor on the Y chromosome in the fish Oryzias dancena (2014)
  • Author(s): Takehana, Y., Matsuda, M., Myosho, T., Suster, M.L., Kawakami, K., Shin-I, T., Kohara, Y., Kuroki, Y., Toyoda, A., Fujiyama, A., Hamaguchi, S., Sakaizumi, M., and Naruse, K.
  • Journal Title: Nature Communications Volume: 5 Issue: 1 Pages: 4157-4157
  • DOI: 10.1038/ncomms5157
  • Peer Reviewed / Open Access
• [Presentation] Evolution of the sex-determining gene in fish (2014)
  • Author(s): Matsuda, M.
  • Organizer: Internatinal Conference on Frontiers in Comparative Endocrinology and Nueobiology 2014
  • Place of Presentation: Hyderabad, India
  • Year and Date: 2014-11-25 – 2014-11-28
  • Invited
• [Presentation] XX メダカの性転換誘導に おける性決定関連遺伝子 Gsdf の役割 (2014)
  • Author(s): 佐藤忠, 今井拓人, 松田勝, 酒泉満, 濱口哲
  • Organizer: 日本動物学会第85回大会
  • Place of Presentation: 仙台
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] The involvement of Gsdf in sex determination of the medaka, Oryzias latipes (2014)
  • Author(s): Imai, T., Saino, K., Matsuda, M.
  • Organizer: 2nd Strategical Meeting for Medaka Research
  • Place of Presentation: Seville, Spain
  • Year and Date: 2014-04-10 – 2014-04-12
• [Presentation] Gsdfの変異はXYメダカを雄へと性転換させる (2013)
  • Author(s): 今井拓人, 齊野兼太郎, 松田勝
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山
• [Presentation] XX性転換変異体メダカの解析 (2012)
  • Author(s): 今井拓人, 松島美喜子, 松田勝
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪
• [Remarks] 宇都宮大学バイオサイエンス教育研究センター教員HP
  • URL: http://c-bio.mine.utsunomiya-u.ac.jp/lab/matsuda.html