Structure and mechanism of bacterial flagella rod

• Project/Area Number: 24570132
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Osaka University
• Principal Investigator: SAIJO-HAMANO Yumiko 大阪大学, 生命機能研究科, 特任助教 (00444513)
• Co-Investigator(Renkei-kenkyūsha): IMADA Katsumi 大阪大学, 大学院理学研究科, 教授 (40346143) ; KATO Takayuki 大阪大学, 大学院生命機能研究科, 助教 (20423155)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 結晶構造解析 / 細菌べん毛 / ナノマシン / ロッド / X線結晶構造解析

Outline of Final Research Achievements

Bacterial flagellum is a highly efficient nano-machine rotating at high speed, 300 rpm/sec if Salmonella, in viscous environments. The rod of the bacterial flagellum acts as the drive shaft to enable the high-speed rotation. FlgG is the protein to constructs the distal rod which is a major part of the rod. We revealed the molecular model at 2 angstrom resolution of a core fragment of FlgG by X-ray crystal structure analysis. Furthermore we constructed the distal rod structure based on this FlgG model to fit to cryo-EM density map.

Research Products

• [Journal Article] Ｉｄｅｎｔｉｆｉｃａｔｉｏｎ and functional analysis of 2-hydroxyflavanone C-glucosyltransferase in soybean (Glycine max) (2015)
  • Author(s): Yoshihiro Hirade, Naoyuki Kotoku, Kazuyoshi Terasaka, Yumiko Saijo-Hamano, Akemi Fukumoto, Hajime Mizukami
  • Journal Title: FEBS Letters Volume: 未定 Issue: 15 Pages: 1778-1786
  • DOI: 10.1016/j.febslet.2015.05.010
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Conformational analysis of isolated domains of Helicobacter pylori CagA. (2013)
  • Author(s): Woon, A.-P., Tohidpour, A., Alonso, H., Saijo-Hamano, Y., Kwok, T. & Roujeinikova, A.
  • Journal Title: PLOS One Volume: 8 Issue: 11 Pages: e79367-e79367
  • DOI: 10.1371/journal.pone.0079367
  • Peer Reviewed
• [Journal Article] Expression, purification, crystallization and preliminary X-ray diffraction analysis of a core fragment of FlgG, a bacterial flagellar rod protein (2013)
  • Author(s): Yumiko Saijo-Hamano*, Hideyuki Matsunami, Keiichi Namba and Katsumi Imada
  • Journal Title: Acta Crystallographica Section F Volume: F69 Issue: 5 Pages: 547-550
  • DOI: 10.1107/s1744309113008075
  • Peer Reviewed
• [Journal Article] Isolation of Salmonella mutants resistant to the inhibitory effect of salicylidene acylhydrazides on flagella-mediated motility. (2013)
  • Author(s): Martinez-Argudo, I., et al
  • Journal Title: PLoS One Volume: 8 Issue: 1 Pages: e52179-e52179
  • DOI: 10.1371/journal.pone.0052179
  • Peer Reviewed
• [Presentation] 細菌べん毛ディスタルロッドの構造解析. High-resolution structure of the bacterial flagellar distal rod.
  • Author(s): 西條由見子, 今田勝巳, 松波秀行, 藤井高志, 難波啓一.
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] 細菌べん毛ディスタルロッドの構造と力学的特性
  • Author(s): 西條由見子, 今田勝巳, 松波秀行, 藤井高志, 難波啓一.
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県）
• [Presentation] 細菌べん毛ロッドタンパク質の結晶化（アミロイド化させずに結晶化させる）
  • Author(s): 西條由見子, 松波秀行, 難波啓一, 今田勝巳.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡（福岡県）
• [Presentation] サルモネラ菌べん毛ロッドタンパク質FlgGのコア領域の結晶構造解析（結晶化の話を中心に）
  • Author(s): 西條由見子
  • Organizer: 2012年度べん毛研究交流会
  • Place of Presentation: よろこびの宿 しん喜（群馬県）
• [Presentation] 細菌べん毛ロッドタンパク質の結晶化の試み -アミロイド化させずに結晶化させる-
  • Author(s): 西條由見子
  • Organizer: 第3回生命機能研究会
  • Place of Presentation: 兵衛向陽閣（兵庫県）
  • Invited
• [Remarks] プロトニックナノマシン研究室〈難波教授〉
  • URL: http://www.fbs.osaka-u.ac.jp/jpn/general/lab/02/result/