Resolving the function of the ENH1-PKC/PKD-Calcium Channel function in the cardiac hypertrophy
| Project/Area Number |
24570150
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
MATURANA ANDRES 名古屋大学, 生命農学研究科, 准教授 (10452004)
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| Co-Investigator(Renkei-kenkyūsha) |
KURODA Shunichi 名古屋大学, 大学院生命農学研究科, 教授 (60263406)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Keywords | シグナル伝達 / 心臓肥大 / intracellular signaling / scaffolding protein / cardiac hypetrophy / PDZ LIM protin / 細胞内シグナル / Zでぃすく / 足場タンパク質 / Zディスク |
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| Outline of Final Research Achievements |
Scaffolding proteins are essential for the transmission of intracellular signaling. Scaffolding proteins gather signaling proteins and their targets at precise subcellular locations to maximize the efficiency of the signal transduction. We are studying a scaffolding protein called ENH that is essential for the cardiac and skeletal muscle development. We have previously shown that ENH is essential for the development of cardiac hypertrophy but some ENH splice variants can on the contrary prevent cardiac hypertrophy. In the present study, we studied the splicing mechanisms of ENH. We generate a splice variant ENH trangenic mice to study in-vivo its ability to prevent cardiac hypertrophy. Finally, we analyzed the downstream signaling events downstream to the complex signaling composed by ENH1, the protein kinase D and the L-type calcium channels. Our work sustain the idea that ENH is a key player in the cardiac remodeling leading to heart hypertrophy.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Structural Basis for the Counter-Transport Mechanism of a H+/Ca2+ Exchanger2013
Author(s)
Nishizawa T, Kita S, Maturana AD, Furuya N, Hirata K, Kasuya G, Ogasawara S, Dohmae N, Iwamoto T, Ishitani R, Nureki O
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Journal Title
Science
Volume: 341
Issue: 6142
Pages: 168-172
DOI
Related Report
Peer Reviewed
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[Journal Article] An automated system for high-throughput single cell-based breeding2013
Author(s)
Nobuo Yoshimoto, Akiko Kida, Xu Jie, Masaya Kurokawa, Masumi Iijima, Tomoaki Niimi, Andrés D. Maturana, Itoshi Nikaido, Hiroki R. Ueda, Kenji Tatematsu, Katsuyuki Tanizawa, Akihiko Kondo, Ikuo Fujii, Shun'ichi Kuroda
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Journal Title
Scientific Reports
Volume: 3
Issue: 1
Pages: 1191-1191
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Structural basis for the drug extrusion mechanism by a MATE multidrug transporter2013
Author(s)
Y・ Tanaka, C・J・ Hipolito, A・D・ Maturana, K・ Ito, T・ Kuroda, T・ Higuchi, T・ Katoh, H・E・ Kato, M・ Hattori, K・ Kumazaki, T・ Tsukazaki, R・ Ishitani, H・ Suga, O・ Nureki
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Journal Title
Nature
Volume: 496
Issue: 7444
Pages: 247-51
DOI
Related Report
Peer Reviewed
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