Metallomics study for the enhanced thermal stability of membrane proteins
Project/Area Number |
24570158
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Functional biochemistry
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Research Institution | Kobe University |
Principal Investigator |
KIMURA YUKIHIRO 神戸大学, (連合)農学研究科(研究院), 助教 (20321755)
|
Co-Investigator(Renkei-kenkyūsha) |
OTOMO Seiu 茨城大学, 理学部, 教授 (10213612)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
|
Keywords | 光合成細菌 / 膜タンパク質 / 耐熱性 / カルシウム / 全反射吸収赤外分光法 / 等温滴定熱量分析 / 共鳴ラマン分光法 / 原子吸光分光法 / 光捕集タンパク質 / 赤外分光法 / ラマン分光法 / 熱量分析 / 金属タンパク質 / 光合成 / ストロンチウム |
Outline of Final Research Achievements |
Purple sulfur photosynthetic bacterium Thermochromatium (Tch.) tepidum grows at the highest known temperature among purple bacteria. The light-harvesting 1 reaction center comples (LH1-RC) enhances the thermal stability by utilizing calcium ions. In the present study, metal-protein and metal-pigment interactions and metal-binding sites of the LH1-RC complex from Tch. tepidum were investigated by means of various physicohemical analyses. The results provided valuable information for understanding the moelcular mechanism responsible for the enhanced thermal stability of the Tch. tepidum LH1-RC complex.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] The Roles of C-terminal Residues on the Thermal Stability and Local Heme Environment of Cytochrome c' from the Thermophilic Purple Sulfur Bacterium Thermochromatium2015
Author(s)
Kimura, Y., Kasuga, S., Unno, M., Furusawa, T., Osoegawa, S., Sasaki, Y., Ohno, T., and Wang-Otomo, Z.-Y.
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Journal Title
Photosynthesis Research
Volume: 124
Issue: 1
Pages: 19-29
DOI
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Peer Reviewed / Acknowledgement Compliant
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[Presentation] Molecular mechanisms for the enhanced thermal stability and unusually red-shifted Qy transition of light-harvesting 1 complexes from thermophilic purple sulfur bacterium Thermochromatium tepidum as revealed by vibrational spectroscopy2013
Author(s)
Kimura, Y., Yong Li, Arikawa, T., Inada, Y., Wang, Z.-Y., and Ohno, T.
Organizer
16th International Congress on Photosynthesis
Place of Presentation
Hyatt Regency at The Arch
Related Report
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[Presentation] Detection of pigment-protein interactions in photosynthetic purple bacteria by Near Infrared Raman Spectroscopy2012
Author(s)
Mumata, T., Inada, Y., Yong Li, Arikawa, T., Wang, Z.-Y., Ohno, T., and Kimura, Y.
Organizer
Japan Analytical & Scientific Instruments Show
Place of Presentation
幕張メッセ国際展示場
Related Report
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