Metallomics study for the enhanced thermal stability of membrane proteins

• Project/Area Number: 24570158
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Kobe University
• Principal Investigator: KIMURA YUKIHIRO 神戸大学, (連合)農学研究科(研究院), 助教 (20321755)
• Co-Investigator(Renkei-kenkyūsha): OTOMO Seiu 茨城大学, 理学部, 教授 (10213612)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: 光合成細菌 / 膜タンパク質 / 耐熱性 / カルシウム / 全反射吸収赤外分光法 / 等温滴定熱量分析 / 共鳴ラマン分光法 / 原子吸光分光法 / 光捕集タンパク質 / 赤外分光法 / ラマン分光法 / 熱量分析 / 金属タンパク質 / 光合成 / ストロンチウム

Outline of Final Research Achievements

Purple sulfur photosynthetic bacterium Thermochromatium (Tch.) tepidum grows at the highest known temperature among purple bacteria. The light-harvesting 1 reaction center comples (LH1-RC) enhances the thermal stability by utilizing calcium ions. In the present study, metal-protein and metal-pigment interactions and metal-binding sites of the LH1-RC complex from Tch. tepidum were investigated by means of various physicohemical analyses. The results provided valuable information for understanding the moelcular mechanism responsible for the enhanced thermal stability of the Tch. tepidum LH1-RC complex.

Research Products

• [Journal Article] The Roles of C-terminal Residues on the Thermal Stability and Local Heme Environment of Cytochrome c' from the Thermophilic Purple Sulfur Bacterium Thermochromatium (2015)
  • Author(s): Kimura, Y., Kasuga, S., Unno, M., Furusawa, T., Osoegawa, S., Sasaki, Y., Ohno, T., and Wang-Otomo, Z.-Y.
  • Journal Title: Photosynthesis Research Volume: 124 Issue: 1 Pages: 19-29
  • DOI: 10.1007/s11120-014-0069-6
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] ATR–FTIR Detection of Metal-Sensitive Structural Changes in the Light-Harvesting 1 Reaction Center Complex from the Thermophilic Purple Sulfur Bacterium Thermochromatium tepidum (2013)
  • Author(s): Yong Li, Yukihiro Kimura, Teruhisa Arikawa, Zheng-Yu Wang-Otomo, and Takashi Ohno
  • Journal Title: Biochemistry Volume: 52 Issue: 50 Pages: 9001-9008
  • DOI: 10.1021/bi401033y
  • Peer Reviewed
• [Journal Article] Strontium ions are functionally replaceable with calcium ions in the light-harvesting 1 reaction center core complex from Thermophilic purple sulfur bacterium Thermochromatium tepidum (2013)
  • Author(s): Yukihiro Kimura, Yuta Inada, Long-Jiang Yu, Takashi Ohno, and Zheng-Yu Wang,
  • Journal Title: Photosynthesis Research for Food, Fuel and the Future, Advanced Topics in Science and Technology in China Volume: VI Pages: 105-109
• [Presentation] Thermodynamic analysis of metal-protein interaction in the light-harvesting 1 reaction center complex from purple sulfur bacteria (2014)
  • Author(s): Kimura, Y., Hayashi, Y., Wang, Z.-Y., and Ohno, T.
  • Organizer: The 52nd Annual Meeting of the BSJ
  • Place of Presentation: Sapporo Convention Center
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Isotope-edited ATR-FTIR analysis of light-harvesting 1 reaction center complex from thermophilic purple photosynthetic bacteria (2014)
  • Author(s): Yura, Y., Kimura, Y., Wang, Z.-Y., and Ohno, T.
  • Organizer: The 52nd Annual Meeting of the BSJ
  • Place of Presentation: Sapporo Convention Center
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 同位体置換された好熱性紅色細菌Thermochromatium tepidum由来光捕集１反応中心複合体の振動分光学的解析 (2014)
  • Author(s): 木村行宏、由良優季、永麗、大友征宇、大野隆
  • Organizer: 第22回光合成セミナー：反応中心と色素系の多様性
  • Place of Presentation: 名古屋工業大学
  • Year and Date: 2014-07-12 – 2014-07-13
• [Presentation] 好熱性紅色細菌由来光捕集１反応中心複合体のメタロミクス解析 (2014)
  • Author(s): 林勇介、木村行宏、大友征宇、大野隆
  • Organizer: 第22回光合成セミナー：反応中心と色素系の多様性
  • Place of Presentation: 名古屋工業大学
  • Year and Date: 2014-07-12 – 2014-07-13
• [Presentation] Metal cations modulate protein conformation and thermal stability of light-harvesting 1 reaction center complexes from purple sulfur photosynthetic bacteria (2014)
  • Author(s): Kimura, Y., Yura, Y., Yong Li, Wang, Z.-Y., and Ohno T.
  • Organizer: The 2nd Awaji International Workshop on Electron Spin Science & Technology
  • Place of Presentation: Awaji Yumebutai
  • Year and Date: 2014-06-15 – 2014-06-17
  • Invited
• [Presentation] Molecular mechanisms for the enhanced thermal stability and unusually red-shifted Qy transition of light-harvesting 1 complexes from thermophilic purple sulfur bacterium Thermochromatium tepidum as revealed by vibrational spectroscopy (2013)
  • Author(s): Kimura, Y., Yong Li, Arikawa, T., Inada, Y., Wang, Z.-Y., and Ohno, T.
  • Organizer: 16th International Congress on Photosynthesis
  • Place of Presentation: Hyatt Regency at The Arch
• [Presentation] 好熱性紅色細菌由来光捕集１反応中心複合体の耐熱化を制御する構造変化の振動分光学的検出 (2013)
  • Author(s): 木村行宏、永麗、有川曜央、大友征宇、大野隆
  • Organizer: 第２１回光合成の色素系と反応中心に関するセミナー
  • Place of Presentation: 名古屋工業大学
• [Presentation] 全反射吸収赤外分光法による紅色硫黄光合成細菌Thermochromatium tepidum由来光捕集1膜蛋白質複合体の構造解析 (2013)
  • Author(s): 永麗、木村行宏、大友征宇、大野隆
  • Organizer: 日本化学会第93春季年会
  • Place of Presentation: 立命館大学びわこ・くさつキャンパ
• [Presentation] 灌流誘起赤外分光法による光合成膜蛋白質複合体の構造解析 (2012)
  • Author(s): 永麗、木村行宏、大野隆
  • Organizer: 第477回日本農芸化関西支部講演会
  • Place of Presentation: 神戸大学 六甲台キャンパス
• [Presentation] 紅色硫黄細菌由来光捕集１複合体における色素ー蛋白質相互作用の解析 (2012)
  • Author(s): 木村行宏、稲田勇太、有川曜央、沼田朋子、大友征宇、大野隆
  • Organizer: 第20回光合成の色素系と反応中心に関するセミナー
  • Place of Presentation: 大阪大学 豊中キャンパス
• [Presentation] 好熱性紅色硫黄細菌由来光捕集1複合体におけるBChl-aとTrp残基間の水素結合相互作用 (2012)
  • Author(s): 永麗、木村行宏、沼田朋子、稲田勇太、有川曜央、大友征宇、大野隆
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋大学東山キャンパス
• [Presentation] Detection of pigment-protein interactions in photosynthetic purple bacteria by Near Infrared Raman Spectroscopy (2012)
  • Author(s): Mumata, T., Inada, Y., Yong Li, Arikawa, T., Wang, Z.-Y., Ohno, T., and Kimura, Y.
  • Organizer: Japan Analytical & Scientific Instruments Show
  • Place of Presentation: 幕張メッセ国際展示場
• [Remarks]
  • URL: http://www.edu.kobe-u.ac.jp/ans-bpc/