| Project/Area Number |
24570161
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Okayama University |
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Principal Investigator |
Mori Koichi 岡山大学, 自然科学研究科, 助教 (50379715)
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| Co-Investigator(Kenkyū-buntansha) |
TOBIMATSU Takamasa 岡山大学, 大学院自然科学研究科, 准教授 (30188768)
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| Co-Investigator(Renkei-kenkyūsha) |
TORAYA Tetsuo 岡山大学, 名誉教授 (70026318)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | ビタミンB12 / 補酵素B12 / ラジカル触媒酵素 / 再活性化因子 / 再活性化蛋白質 / メチルマロン酸尿症 / B12アデノシル化酵素 / 酵素反応機構 / ビタミンB12補酵素 / メチルマロニルCoAムターゼ / MMAA蛋白質 / ジオールデヒドラターゼ / グリセロールデヒドラターゼ / エタノールアミンアンモニアリアーゼ / アデノシルコバラミン / 酵素の不活性化と再活性化 / コバラミン / 酵素の再活性化 |
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| Outline of Final Research Achievements |
Vitamin B12 is biologically converted to the coenzyme form, and participate in enzyme reactions. Coenzyme B12-dependent enzymes catalyze enzymatically difficult reactions by radical mechanism. On the other hand, coenzyme B12-dependent enzymes tend to inactivate accompanying side reactions. In order to overcome such inactivation, several coenzyme B12-dependent enzymes have specific reactivating system for an inactivated enzyme. In human, a malfunction of a coenzyme B12-dependent enzyme leads to metabolic disorder. Also, inactivation of enzyme will be a serious problem when it applied to industrial production. Furthermore, since vitamin B12 is a rare compound, efficient use is desired.
In this research project, we studied on proteins that participate in reactivation of coenzyme B12-dependent enzymes and recycling of coenzyme B12 in order to elucidate systems that maintain catalytic activity of coenzyme B12-dependent radical enzymes.
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