Molecular mechanism of endocytosis by a novel membrane-bending domain
| Project/Area Number |
24570216
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Cell biology
|
|---|
| Research Institution | Kobe University |
|---|
Principal Investigator |
ITOH TOSHIKI 神戸大学, 学内共同利用施設等, 教授 (30313092)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
TSUJITA Kazuya 神戸大学, 自然科学系先端融合研究環バイオシグナル研究センター, 助教 (10457054)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | エンドサイトーシス / イノシトールリン脂質 / 生体膜 |
|---|
| Outline of Final Research Achievements |
It was found that human SH3YL1, which contains a novel phosphoinositide-binding domain named the SYLF domain, is involved in endocytosis of epidermal growth factor receptor. In vitro, SH3YL1 not only bound to liposomes, but also mediated interactions between liposomes that it resides, promoting aggregation of membrane vesicles. This study also succeeded in obtaining a knowledge about the positively-charged residues in the SYLF domain that participate in lipid-binding, therefore will provide a novel molecular basis of endocytosis.
|
Report
(4 results)
Research Products
(23 results)
-
[Journal Article] The functional link between Rab GTPase-mediated membrane trafficking and PI4,5P_2 signaling.2014
Author(s)
Cuifang Li, Ayako Kita, Yuuka Hashimoto, Misako Ihara, Ayaka Kato, Naoya Ogura, Akira Doi, Masahide Oku, Toshiki Itoh, Yasuyoshi Sakai, Reiko Sugiura
-
Journal Title
Genes to Cells
Volume: VOL. 19
Issue: 3
Pages: 177-197
DOI
Related Report
Peer Reviewed
-
[Journal Article] Phosphatidylinositol 4-phosphate in the Golgi apparatus regulates cell-cell adhesion and invasive cell migration in human breast cancer.2014
Author(s)
Tokuda, E., Itoh, T., Hasegawa, J., Ijuin, T., Takeuchi, Y., Irino, Y., Fukumoto, M., Takenawa, T.
-
Journal Title
Cancer Res.
Volume: 74
Issue: 11
Pages: 3054-3066
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
