Analysis of rice genes involved in RNA silencing and response to virus

• Project/Area Number: 24580065
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Ehime University
• Principal Investigator: Nishiguchi Masamichi 愛媛大学, 農学部, 研究員 (40343313)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: RNAサイレンシング / イネ / 防御 / イネえそモザイクウイルス / キュウリモザイクウイルス / DNA損傷 / 小分子RNA / 感染応答 / RNA サイレンシング / 遺伝子サイレンシング / ウイルス病 / 菌類病 / 細菌病 / RDR6 / SGS3 / ジーンサイレンシング / RNA依存RNAポリメラーゼ / ウイルス / 遺伝子 サイレンシング / 植物病防御 / 植物 / siRNA / miRNA / qiRNA

Outline of Final Research Achievements

Defense gens including lipoxygenase were proven to be down-regulated in an OsRDR1 mutant by rice 44K microarray. Using rice mutants and overexpressed lines, response to infection with virus, bacterial and fungal pathogens were analyzed. All mutants of OsRDR1, OsRDR6 and OsSGS3 were found to be more sensitive to pathogen infection, respectively, than wild type while the overexpressed lines of OsRDR1 or OSSGS3 showed resistance. DNA damaging stress induced rice to produce DNA damage specific small RNAs. Both OsRDR1 and OsRecQ1 were elucidated to be involved in this small RNA biogenesis.

Research Products

• [Journal Article] Analysis of rice RNA-dependent RNA polymerase (OsRDR6) gene in response to viral, bacterial and fungal pathogens (2016)
  • Author(s): Wagh S. G., Alam M. M., Kobayashi K., Yaeno T., Yamaoka N., Toriba T., Hirano H.-Y. and Nishiguchi M.
  • Journal Title: Journal of General Plant Pathology Volume: 82 Issue: 1 Pages: 12-17
  • DOI: 10.1007/s10327-015-0630-y
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Conferring high-temperature tolerance to nontransgenic tomato scions using graft transmission of RNA silencing of the fatty acid desaturase gene. (2016)
  • Author(s): Nakamura S., Hondo K., Kawara T., Okazaki Y., Saito K., Kobayashi K., Yaeno T., Yamaoka N. and Nishiguchi M.
  • Journal Title: Plant Biotech Journal Volume: 14 Issue: 2 Pages: 783-790
  • DOI: 10.1111/pbi.12429
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Rice necrosis mosaic virus, a fungal transmitted Bymovirus: complete nucleotide sequence of the genomic RNAs and subgrouping of bymoviruses. (2016)
  • Author(s): Wagh S. G., Kobayashi K., Yaeno T., Yamaoka N. and Nishiguchi M.
  • Journal Title: Journal of General Plant Pathology Volume: 82 Issue: 1 Pages: 38-42
  • DOI: 10.1007/s10327-015-0618-7
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Transmission of RNA silencing through grafting: conferring virus resistance from transgenically silenced tobacco rootstocks to non-transgenic tomato scions (2015)
  • Author(s): M.A. Emran, S. Waliullah, K. Kobayashi, N. Yamaoka and M. Nishiguchi
  • Journal Title: J Plant Biochem Biotechnol Volume: ｰ Issue: 3 Pages: 245-252
  • DOI: 10.1007/s13562-015-0334-6
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Molecular analysis of an attenuated strain of Cucumber green mottle mosaic virus using in vitro infectious cDNA clone (2015)
  • Author(s): M. E. Ali, S. Waliullah and M. Nishiguchi
  • Journal Title: J Plant Biochem Biotechnol Volume: ｰ Issue: 1 Pages: 79-86
  • DOI: 10.1007/s13562-015-0312-z
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Overexpression of a rice heme activator protein gene (OsHAP2E) confers resistance to pathogens, salinity and drought, and increases photosynthesis and tiller number (2015)
  • Author(s): Md. M. Alam, T. Tanaka, H. Nakamura, H. Ichikawa, K. Kobayashi, T. Yaeno, N. Yamaoka, K. Takayama, H. Nishina and M. Nishiguchi
  • Journal Title: Plant Biotech J Volume: 13 Issue: 1 Pages: 85-96
  • DOI: 10.1111/pbi.12239
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Overexpression of OsHAP2E for a CCAAT-binding factor confers resistance to Cucumber mosaic virus and Rice necrosis mosaic virus (2015)
  • Author(s): Md. M. Alam, H. Nakamura, H. Ichikawa, K. Kobayashi, T. Yaeno, N. Yamaoka and M. Nishiguchi.
  • Journal Title: J Gen Plant Pathol Volume: 81 Issue: 1 Pages: 32-41
  • DOI: 10.1007/s10327-014-0564-9
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Response of an aspartic protease gene OsAP77 to fungal, bacterial and viral infections in rice (2014)
  • Author(s): Md. M. Alam, H. Nakamura, H. Ichikawa, A. Miyao, H. Hirochika, K. Kobayashi, N. Yamaoka and M. Nishiguchi
  • Journal Title: RICE Volume: 7 Issue: 1 Pages: 1-13
  • DOI: 10.1186/s12284-014-0009-2
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Artificial induction of a plant virus protein in transgenic tobacco provides a synchronous system for analyzing the process of leaf chlorosis (2014)
  • Author(s): S. Waliullah, T. Mochizuki, K.-T. Sekine, G. Atsumi , M. E. Ali, T. Yaeno, N. Yamaoka, M. Nishiguchi and K. Kobayashi.
  • Journal Title: Physiol Mol Plant Pathol Volume: ８８ Pages: 43-51
  • DOI: 10.1016/j.pmpp.2014.08.005
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Recent studies on biological control of plant diseases in Japan (2014)
  • Author(s): M. Hyakumachi, H. Takahashi, Y. Matsubara, N. Someya, M. Shimizu, K. Kobayashi, M. Nishiguchi
  • Journal Title: Journal of General Plant Pathology Volume: XX
  • NAID: 130004779396
  • Peer Reviewed
• [Journal Article] Graft transmission of RNA silencing to non-transgenic scions for conferring virus resistance in tobacco. (2013)
  • Author(s): E Md Ali, K Kobayashi, N Yamaoka, M. Ishikawa, M. Nishiguchi.
  • Journal Title: PLOS ONE Volume: 8(5): e63257 Issue: 5 Pages: 1-8
  • DOI: 10.1371/journal.pone.0063257
  • Peer Reviewed
• [Journal Article] Both OsRecQ1 and OsRDR1 are required for the production of small RNA in response to DNA-damage in rice (2013)
  • Author(s): H. Chen, K. Kobayashi, A. Miyao, H. Hirochika, N. Yamaoka and M. Nishiguchi.
  • Journal Title: PLOS ONE Volume: 8: e55252 Issue: 1 Pages: 1-8
  • DOI: 10.1371/journal.pone.0055252
  • Peer Reviewed
• [Presentation] Analysis of Transgenic Rice Overexpressing a Truncated Form of a Rice Receptor-like Kinase Gene, Leaf Panicle 2: Virus Resistance (2016)
  • Author(s): M. M. Alam, Y. Uemura, N. Hara, S. Toki, K. Kobayshi, T. Yaeno, N. Yamaoka and M. Nishiguchi
  • Organizer: 平成２8年度日本植物病理学会大会
  • Place of Presentation: 岡山コンベンションセンター（岡山県岡山市）
  • Year and Date: 2016-03-21
• [Presentation] Functional analysis of Beet pseudo-yellows virus RNA1 encoded-p6 gene (2016)
  • Author(s): Akhter, M.S., Bhor S.A., Yaeno, T., Yamaoka, N., Nishiguchi, M. and Kobayashi, K.
  • Organizer: 平成２8年度日本植物病理学会大会
  • Place of Presentation: 岡山コンベンションセンター（岡山県岡山市）
  • Year and Date: 2016-03-21
• [Presentation] Artificial induction of chlorosis symptom through the silencing of chloroplast genes in transgenic tobacco (2016)
  • Author(s): Bhor, S.A., Sekine, K.-T.1, Yaeno, T., Yamaoka, N., Nishiguchi, M. and Kobayashi, K.
  • Organizer: 平成２8年度日本植物病理学会大会
  • Place of Presentation: 岡山コンベンションセンター（岡山県岡山市）
  • Year and Date: 2016-03-21
• [Presentation] Complete nucleotide sequences of a fungus transmitted Bymovirus, Rice necrosis mosaic virus genomic RNAs and comparison with those of other members (2015)
  • Author(s): Wagh S. G., Kobayashi, K., Yaeno T., Yamaoka, N., Masuta, C. and M. Nishiguchi
  • Organizer: 平成２７年度日本植物病理学会関西部会
  • Place of Presentation: あわぎんホール（徳島県徳島市）
  • Year and Date: 2015-09-29
• [Presentation] 薬剤誘導型カリフラワーモザイクウイルスTav遺伝子導入シロイヌナズナは誘導薬剤依存的に葉の黄化および成長抑制を示す (2015)
  • Author(s): 寺田忍・B. S. Ashok・関根健太郎・八丈野孝・山岡直人・西口正通・小林括平
  • Organizer: 平成２７年度日本植物病理学会関西部会
  • Place of Presentation: あわぎんホール（徳島県徳島市）
  • Year and Date: 2015-09-29
• [Presentation] Construction of a single copy agroinfection vector for the development of infectious cDNA clone of plant viruses (2015)
  • Author(s): Akhter, M.S., Sekine, K.-T.1, Yaeno, T., Yamaoka, N., Nishiguchi, M. and Kobayashi, K.
  • Organizer: 平成２７年度日本植物病理学会関西部会
  • Place of Presentation: あわぎんホール（徳島県徳島市）
  • Year and Date: 2015-09-29
• [Presentation] Genome sequence analysis of Rice necrosis mosaic virus, a fungus transmitted Bymovirus (2015)
  • Author(s): G. S. Wagh, K. Kobayashi, T. Yaeno, N. Yamaoka and M. Nishiguchi
  • Organizer: 第30回中国四国ウイルス研究会
  • Place of Presentation: 岡山大学（岡山県倉敷市）
  • Year and Date: 2015-06-27
• [Presentation] Roles of Rice RNA Dependent RNA Polymerase (OsRDR1 anOsRDR6) to Viral, Fungal and Bacterial Infections (2015)
  • Author(s): Wagh, S. G., M. Alam, M., Tanaka, T., Miyao, A., H. Hirochika, H., Toba, T., Hirano, H., Kobayashi, K., Yaeno T., Yamaoka, N. and Nishiguchi, M.
  • Organizer: 平成27年度日本植物病理学会大会
  • Place of Presentation: 東京都
  • Year and Date: 2015-03-28 – 2015-03-31
• [Presentation] イネRNAサイレンシング関与遺伝子OsSGS3の機能解析 (2015)
  • Author(s): 田中 徹・ Wagh, S. G.・Alam, M. M.・Chen, H.・宮尾安藝雄・廣近 洋彦・小林括平・八丈野孝・山岡直人・西口正通
  • Organizer: 平成27年度日本植物病理学会大会
  • Place of Presentation: 東京都
  • Year and Date: 2015-03-28 – 2015-03-31
• [Presentation] A rice activator protein gene (OsHAP2E), a CCAAT binding protein, plays multiple roles in rice: resistance to biotic and abiotic stress and incleased photosynthesis and tiller number (2014)
  • Author(s): Alam, M.M., K. Kobayashi, T. Yaeno, N. Yamaoka and M. Nishiguchi
  • Organizer: 第37 回日本分子生物学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Role of rice hemeactivator protein gene (OsHAP2E), a CCAAT binding protein: resistance to fugi, bacterial and viral pathogens. (2014)
  • Author(s): Alam, M.M., K. Kobayashi, T. Yaeno, N. Yamaoka and M. Nishiguchi
  • Organizer: 3rd Korea-Japan Joint Symposium
  • Place of Presentation: Busan, Korea
  • Year and Date: 2014-10-22 – 2014-10-25
• [Presentation] Virus Resistance in Transgenic Rice Over-expressing a Heme Activator Protein Gene (OsHAP2E): Rice necrosis mosaic virus (2014)
  • Author(s): Alam, M.M., K. Kobayashi, T. Yaeno, N. Yamaoka and M. Nishiguchi
  • Organizer: 平成26年度日本植物病理学会関西部会
  • Place of Presentation: 富山市
  • Year and Date: 2014-09-27 – 2014-09-28
• [Presentation] Response of Rice RNA dependent RNA Polymerase1 (OsRDR1) and Suppressor of Gene Silencing (OsSGS3) to Pathogen Infections. (2014)
  • Author(s): Wagh, S.G., M.M. Alam, T. Tanaka, K. Kobayashi, T. Yaeno, N. Yamaoka and M. Nishiguchi
  • Organizer: 平成26 年度日本植物病理学会関西部会
  • Place of Presentation: 富山市
  • Year and Date: 2014-09-27 – 2014-09-28
• [Presentation] Resistance to Rice necrosis mosaic virus in rice overexpressing OsHAP2E, a rice CAAT binding protein gene (2014)
  • Author(s): Alam, M.M., T. Tanaka, M.E. Ali, K. Kobayashi, T. Yaeno, N. Yamaoka and M. Nishiguchi
  • Organizer: 第29 回中国四国ウイルス研究会
  • Place of Presentation: 山口市
  • Year and Date: 2014-06-28 – 2014-06-29
• [Presentation] Molecular Cloning and Nucleotide Sequence of Rice necrosis mosaic virus, a Fungal Transmitted Bymovirus, Genomic RNA (2014)
  • Author(s): Wagh, S.G., M.M. Alam, T. Tanaka, K. Kobayashi, T. Yaeno, N. Yamaoka and M. Nishiguchi
  • Organizer: 平成26年度日本植物病理学会大会
  • Place of Presentation: 札幌市
  • Year and Date: 2014-06-02 – 2014-06-04
• [Presentation] Response of Rice Heme Activator Protein Gene (OsHAP2E) to Virus Infection (2014)
  • Author(s): Alam, M.M., H. Nakamura, H. Ichikawa, K. Kobayashi, T. Yaeno, N. Yamaoka and M. Nishiguchi
  • Organizer: 平成26年度日本植物病理学会大会
  • Place of Presentation: 札幌市
  • Year and Date: 2014-06-02 – 2014-06-04
• [Presentation] Functional characterization of rice aspartic protease (OsAP77) gene in response to bacterial and fungal pathogens (2014)
  • Author(s): Alam M. M., Miyao M. , Hirochika H., Nakamura H., Ichikawa H., Kobayshi K., Yamaoka N., Nishiguchi M.
  • Organizer: Plant and Animal Genome Conference XXII,
  • Place of Presentation: 米国 サンディエゴ
• [Presentation] Further investigation of graft-transmission of RNA silencing and virus resistance from transgenic silenced rootstocks to non-transgenic scions of tobacco and tomato. (2013)
  • Author(s): Nishiguchi M., Ali M. E., Kobayashi K., Yaeno T. and Yamaoka N.
  • Organizer: 第29回日本分子生物学会年会
  • Place of Presentation: 神戸市
• [Presentation] Further investigation on graft-transmission of RNA silencing and tobamovirus resistance to non-transgenic scions of tobacco and tomato. (2013)
  • Author(s): Ali, M. E., Kobayashi, K., Yaeno, T., Yamaoka, N. and Nishiguchi, M.
  • Organizer: 平成25年度日本植物病理学会関西部会
  • Place of Presentation: 岡山市
• [Presentation] Further investigation on graft-transmission of RNA silencing from silenced rootstocks to non-transgenic scions of necrotic responding tobacco upon tobamovirus infection. (2013)
  • Author(s): Ali Md. E., Kobayashi K., Yamaoka N. and Nishiguchi M.
  • Organizer: 第28 回中国四国ウイルス研究会
  • Place of Presentation: 広島市
• [Presentation] OsRecQ1 and OsRDR1 is involved in the biogensis of qiRNA and DNA damage response in rice (2013)
  • Author(s): Chen H., Kobayashi K., Miyao A., Hirochika H., Yamaoka N. and Nishiguchi M. .
  • Organizer: Keystone Symposia on RNA silencing. Abstract Book p56. (2013)
  • Place of Presentation: Whistler, BC, Canada
• [Presentation] The use of Cucumber mosaic virus in rice plants for analysis of stress inducible gene (2013)
  • Author(s): Alam M. M., Kobayashi K., Yamaoka, N. and Nishiguchi, M.
  • Organizer: 平成24年度日本植物病理学会大会
  • Place of Presentation: 岐阜市
• [Presentation] Effect of rice RNA-dependent RNA Polymerase 1 (OsRDR1) on RNA silencing and small RNA regulation (2012)
  • Author(s): Masamichi NISHIGUCHI, Hui CHEN, Kappei KOBAYASHI, Naoto YAMAOKA.
  • Organizer: XV International Congress of Molecular Plant-Microbe Interactions
  • Place of Presentation: 京都市
• [Book] 日本植物病理学会100年史 (2015)
  • Author(s): 百町満朗・土屋健一・西口正通他
  • Total Pages: 397
  • Publisher: 日本植物病理学会
• [Book] 植物防疫 (2015)
  • Author(s): 西口正通・M.M. ALAM・小林括平・市川裕章他
  • Total Pages: 77
  • Publisher: 日本植物防疫協会
• [Book] 生物防除． 日本植物病理学会100周年記念誌 (2015)
  • Author(s): 百町満朗・土屋健一・西口正通.
  • Total Pages: 3
  • Publisher: 日本植物病理学会
• [Book] 最近のわが国における植物病害の生物防除研究. 日植病報80特集号 (2014)
  • Author(s): 百町満朗・高橋英樹・松原陽一・染谷信孝・清水将文・小林括平・西口正通
  • Total Pages: 9
  • Publisher: 日本植物病理学会
• [Book] Recent studies on biological control of plant diseases in Japan. J Gen Plant Pathol (2014)
  • Author(s): 2.M. Hyakumachi, H. Takahashi, Y. Matsubara, N. Someya, M. Shimizu, K. Kobayashi and M. Nishiguchi. Recent studies on biological control of plant diseases in Japan. J Gen Plant Pathol 287-302. DOI 10.1007/s10327-014-0524-4 (2014)
  • Total Pages: 16
  • Publisher: 日本植物病理学会
• [Book] Breakdown of plant virus resistance: can we predict and extend the durability of virus resistance? J Gen Plant Pathol (2014)
  • Author(s): K. Kobayashi, K.-T. Sekine and M. Nishiguchi.
  • Total Pages: 10
  • Publisher: 日本植物病理学会
• [Book] 植物ウイルス抵抗性の打破：ウイルス抵抗性の永続性を予測したり延長させたりできるだろうか？日植病報80特集号 (2014)
  • Author(s): 小林括平・関根健太郎・西口正通
  • Total Pages: 7
  • Publisher: 日本植物病理学会
• [Remarks] 研究業績
  • URL: http://web.agr.ehime-u.ac.jp/~bunshishigen/
• [Remarks] 分子生物資源学
  • URL: http://web.agr.ehime-u.ac.jp/~bunshishigen/index.html
• [Remarks] 研究業績
  • URL: http://web.agr.ehime-u.ac.jp/~bunshishigen/index.html
• [Remarks] 研究のトピック
  • URL: http://web.agr.ehime-u.ac.jp/~bunshishigen/index.html
• [Remarks] 遺伝子サイレンシング利用
  • URL: http://web.agr.ehime-u.ac.jp/~bunshishigen/nishiguchi24-inobe-sinki05.pdf
• [Patent(Industrial Property Rights)] 植物に耐病性・耐乾性・耐塩性・光合成効率向上・分げつ数増大を付与する遺伝子 (2014)
  • Inventor(s): 西口正通、エムディ・マフズ・アラム、小林括平
  • Industrial Property Rights Holder: 愛媛大学学長
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-047696
  • Filing Date: 2014-03-11
• [Patent(Industrial Property Rights)] ジーンサイレンシング抑制技術 (2013)
  • Inventor(s): 西口正通、チェン フイ、山岡直人
  • Industrial Property Rights Holder: 愛媛大学学長
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-03-14
  • Acquisition Date: 2013-05-17