Study of functional diversity of plant specific malate transporter, aiming for an increase in crop productivity.

• Project/Area Number: 24580093
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Okayama University
• Principal Investigator: SASAKI TAKAYUKI 岡山大学, 資源植物科学研究所, 助教 (60362985)
• Co-Investigator(Renkei-kenkyūsha): YAMAMOTO Yoko 岡山大学, 資源植物科学研究所, 教授 (50166831)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: リンゴ酸輸送体 / ALMT / アニオン輸送 / アルミニウム / 電気生理学 / ALMT1

Outline of Final Research Achievements

Endomembrane localized ALMT-family transporter in tomato were analyzed. SlALMT possessed malate and anion transport function. The overexpression of the gene could not show malate accumulation in fruits, but caused change of organic anion contents in seed, suggesting that SlALMT transporter is involved in organic anion metabolisms in seed.

Research Products

• [Journal Article] A domain-based approach for analyzing the function of aluminum-activated malate transporters from wheat (Triticum aestivum) and Arabidopsis thaliana in Xenopus oocytes. (2014)
  • Author(s): Sasaki, T., Tsuchiya, Y., Ariyoshi, M., Ryan, P.R., Furuichi, T. and Yamamoto Y.
  • Journal Title: Plant Cell Physiol. Volume: 55 Issue: 12 Pages: 2126-2138
  • DOI: 10.1093/pcp/pcu143
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Sucrose transporter NtSUT1 confers aluminum tolerance on cultured cells of tobacco (Nicotiana tabacum L.). (2013)
  • Author(s): Muhammad S., Sasaki T., Yamamoto Y.
  • Journal Title: Soil Sci. Plant Nutri. Volume: 59 Issue: 5 Pages: 756-770
  • DOI: 10.1080/00380768.2013.830230
  • NAID: 110009766777
  • Peer Reviewed
• [Journal Article] A novel mechanism of aluminium-induced cell death involving vacuolar processing enzyme and vacuolar collapse in tobacco cell line BY-2 (2013)
  • Author(s): Kariya, K., Demiral, T., Sasaki, T., Tsuchiya, Y., Turkan, I., Sano, T., Hasezawa, S., Yamamoto, Y.
  • Journal Title: J. Inorg. Biochem. Volume: 128 Pages: 196-201
  • DOI: 10.1016/j.jinorgbio.2013.07.001
  • Peer Reviewed
• [Journal Article] Al-induced secretion of organic acid, gene expression and root elongation in soybean roots. (2013)
  • Author(s): Wu, D., Zhao, M., Shen, S., Fu, Y., Sasaki, T., Yamamoto, Y., Wei ,W., Shen, H.
  • Journal Title: Acta Physiol. Plant. Volume: 35 Issue: 1 Pages: 223-232
  • DOI: 10.1007/s11738-012-1067-y
  • Peer Reviewed
• [Journal Article] Inhibitory Effects of Methylglyoxal on Light-Induced Stomatal Opening and Inward K<SUP>+</SUP> Channel Activity in <I>Arabidopsis</I> (2012)
  • Author(s): Hoque, T.S., Okuma, E., Uraji, M., Furuichi T., Sasaki, T., Hoque, M.A., Nakamura, Y, Murata, Y.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Issue: 3 Pages: 617-619
  • DOI: 10.1271/bbb.110885
  • NAID: 10030751095
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Presentation] 植物特異的リンゴ酸輸送体ALMTの機能多様性の解析 (2015)
  • Author(s): 佐々木孝行・土屋善幸・有吉美智代・東泉恵美・山本洋子
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 京都
  • Year and Date: 2015-09-09 – 2015-09-11
• [Presentation] タバコの培養細胞ならびに根におけるアルミニウムによる細胞死に伴うVPE遺伝子の発現誘導 (2015)
  • Author(s): 苅谷耕輝・土屋善幸・佐々木孝行・山本洋子
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] アルミニウム耐性タバコ培養細胞株と野生株に発現する遺伝子群の比較解析 (2015)
  • Author(s): 土屋善幸・苅谷耕輝・佐々木孝行・山本洋子
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] タバコのアルミニウムによる根生育阻害における液胞の関わり (2014)
  • Author(s): 苅谷耕輝・土屋善幸・佐々木孝行・山本洋子
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 東京
  • Year and Date: 2014-09-09 – 2014-09-11
• [Presentation] タバコ培養細胞におけるアルミニウム耐性とスクロースシンターゼとの関係 (2014)
  • Author(s): 土屋善幸・苅谷耕輝・佐々木孝行・山本洋子
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 東京
  • Year and Date: 2014-09-09 – 2014-09-11
• [Presentation] ミヤコグサ根粒で発現するALMTの機能解析 (2014)
  • Author(s): 高梨功次郎・佐々木孝行・管智弘・齊田有桂・杉山暁史・山本洋子・矢﨑一史
  • Organizer: 日本植物細胞分子生物学会
  • Place of Presentation: 盛岡
  • Year and Date: 2014-08-21 – 2014-08-22
• [Presentation] Analyses of Aluminum-Activation for ALMT Transporters. (2014)
  • Author(s): Sasaki, T., Furuichi, T., Yamamoto, Y.
  • Organizer: XVII. International Plant Nutrition Colloquium
  • Place of Presentation: Istanbul, Turkey
• [Presentation] Auxin inducible sucrose transpoter NtSUT1 is involved in aluminum tolerance in tobacco cells. (2014)
  • Author(s): Sameeullah, M., Sasaki, T., Yamamoto, Y.
  • Organizer: XVII. International Plant Nutrition Colloquium
  • Place of Presentation: Istanbul, Turkey
• [Presentation] ALMT1輸送体のアルミニウムによる活性化におけるN末端側とC末端側領域の機能解析 (2014)
  • Author(s): 佐々木孝行, 土屋善幸, 有吉美智代, 古市卓也, 山本洋子
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山
• [Presentation] タバコBY-2細胞におけるアルミニウムによるVPE遺伝子の発現誘導と細胞死 (2014)
  • Author(s): 苅谷耕輝, 土屋善幸, 佐々木孝行, 山本洋子
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山
• [Presentation] アルミニウム活性化型ALMT1輸送体の機能向上を目指した遺伝子改変 (2013)
  • Author(s): 佐々木孝行, 古市卓也, 山本洋子
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岡山市
• [Presentation] Role of plasma membrane sucrose transporter in growth in tobacco cells (2013)
  • Author(s): Muhammad Sameeullah, Takayuki Sasaki and Yoko Yamamoto
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岡山市
• [Presentation] BY-2タバコ培養細胞株においてアルミニウムが誘発する細胞死における液胞の関わり (2013)
  • Author(s): 苅谷耕輝、佐々木孝行、山本洋子
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岡山市
• [Presentation] Analyses of aluminum-activation domain of ALMT transporters (2013)
  • Author(s): Takayuki Sasaki, Takuya Furuichi, Yoko Yamamoto
  • Organizer: 16th International Workshop on Plant Membrane Biology
  • Place of Presentation: Kurashiki, Japan
• [Presentation] Effects of major sucrose transporter gene (NtSUT1-l) in normal growth and under aluminum stress in tobacco cells (2013)
  • Author(s): Muhammad Sameeullah, Takayuki Sasaki and Yoko Yamamoto
  • Organizer: 16th International Workshop on Plant Membrane Biology
  • Place of Presentation: Kurashiki, Japan
• [Presentation] AtALMT3 is a malate transporter induced in roots of phosphorus deficient Arabidopsis thaliana (2013)
  • Author(s): Hayato Maruyama, Takayuki Sasaki, Jun Wasaki
  • Organizer: 16th International Workshop on Plant Membrane Biology
  • Place of Presentation: Kurashiki, Japan
• [Presentation] 植物ALMT輸送体機能の比較解析 (2012)
  • Author(s): 佐々木孝行, 有吉美智代,山本洋子
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 鳥取
• [Presentation] Role of sucrose transporter (NtSUT1) under normal growth and Al stress conditions in cultured tobacco cells (2012)
  • Author(s): Muhammad Sameeullah, Takayuki Sasaki and Yoko Yamamoto
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 鳥取
• [Presentation] アルミニウム応答におけるエネルギー代謝の関わり アルミニウム耐性の異なるタバコ培養細胞株の比較解析 (2012)
  • Author(s): 山本洋子、泉洋平、信濃卓郎、中村卓司、岡崎圭毅、佐々木孝行
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 鳥取
• [Presentation] シロバナルーピンのクラスター根から単離したALMTホモログの解析 (2012)
  • Author(s): 矢倉興士、丸山隼人、佐々木孝行、和崎淳
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 鳥取
• [Presentation] タバコ培養細胞BY-2を用いたアルミニウムによる細胞死の解析 (2012)
  • Author(s): 苅谷耕輝、佐々木孝行、山本洋子
  • Organizer: 日本土壌肥料学会 関西支部会
  • Place of Presentation: 倉敷市
• [Presentation] 植物特異的アニオン輸送体の機能多様性と作物生産性向上への応用
  • Author(s): 佐々木孝行
  • Organizer: 平成24年度日本農芸化学会東北支部シンポジウム「遺伝子解析による実用研究の新展開」
  • Place of Presentation: 秋田
  • Invited
• [Remarks] 植物成長制御グループ
  • URL: http://www.rib.okayama-u.ac.jp/plant.growth/index-j.html
• [Remarks] 植物成長制御グループ
  • URL: http://www.rib.okayama-u.ac.jp/plant.growth/index-j.html