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Physiological analysis of polyunsaturated fatty acid (PUFA) in Saccharomyces cerevisiae and its application to improving stress tolerance

Research Project

Project/Area Number 24580109
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionKyoto University

Principal Investigator

TANAKA Koichi  京都大学, (連合)農学研究科(研究院), 研究員 (90282615)

Co-Investigator(Kenkyū-buntansha) SHIMA Jun  龍谷大学, 農学部, 教授 (00343822)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords出芽酵母 / 不飽和脂肪酸 / ストレス耐性 / バイオエタノール / 酢酸 / 微生物 / 脂肪酸 / ステロール / 芳香族アミノ酸
Outline of Final Research Achievements

Lignocellulose hydrolysates contain aliphatic acids, such as acetic acid, that negatively affects the fermentation ability of S. cerevisiae. In this study, we found that the strain overexpressing HAA1 gene acquired a higher level of acetic acid tolerance. The ethanol production ability of HAA1-overexpressing strain was not influenced by acetic acid addition. We believe that this technology has the great potential to reduce bioethanol production costs.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (21 results)

All 2015 2014 2013 2012 Other

All Journal Article (12 results) (of which Peer Reviewed: 6 results,  Open Access: 1 results) Presentation (9 results)

  • [Journal Article] Trehalose accumulation enhances tolerance of Saccharomyces cerevisiae to acetic acid2015

    • Author(s)
      Yoko Yoshiyama, Koichi Tanaka, Kohei Yoshiyama, Makoto Hibi, Jun Ogawa, and Jun Shima
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 119 Issue: 2 Pages: 172-175

    • DOI

      10.1016/j.jbiosc.2014.06.021

    • NAID

      110009922577

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Case for an Early Biological Origin of DNA2014

    • Author(s)
      Anthony M. Poole, Nobuyuki Horinouchi, Ryan J. Catchpole, Dayong Si, Makoto Hibi, Koichi Tanaka, Jun Ogawa
    • Journal Title

      Journal of Molecular Evolution

      Volume: 79 Issue: 5-6 Pages: 204-212

    • DOI

      10.1007/s00239-014-9656-6

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 有機酸ストレス耐性酵母の探索・育種とバイオプロセスへの応用-未利用バイオマスの資源化に向けた酵母の機能開拓2014

    • Author(s)
      島純、田中晃一、中村敏英
    • Journal Title

      化学と生物

      Volume: 52 Pages: 351-353

    • NAID

      130005072109

    • Related Report
      2014 Annual Research Report
  • [Journal Article] 新規有用機能性パン酵母の自然界からの分離と製パンへの応用2014

    • Author(s)
      田中晃一
    • Journal Title

      公益財団法人エリザベス・アーノルド富士財団平成25年度報告書

      Pages: 262-271

    • NAID

      40021273399

    • Related Report
      2014 Annual Research Report
  • [Journal Article] 酵母の環境ストレス耐性機構の解析と食品製造への応用2014

    • Author(s)
      島純、田中晃一、安藤晃規, 小川順
    • Journal Title

      IFO Research Communications

      Volume: 28 Pages: 73-82

    • Related Report
      2014 Annual Research Report
  • [Journal Article] 有機酸ストレス耐性機構の解明と新規バイオ燃料生産プロセスの構築2014

    • Author(s)
      島純、田中晃一、安藤晃規, 小川順
    • Journal Title

      IFO Research Communications

      Volume: 28 Pages: 83-93

    • Related Report
      2014 Annual Research Report
  • [Journal Article] ノンコンベンショナル酵母の機能探索と油脂等の有用物質生産への応用2014

    • Author(s)
      島純、田中晃一、安藤晃規, 小川順
    • Journal Title

      IFO Research Communications

      Volume: 28 Pages: 95-107

    • Related Report
      2014 Annual Research Report
  • [Journal Article] 有機栄養液栽培に寄与する微生物群の解析とその応用2014

    • Author(s)
      安藤晃規、田中晃一、島純、小川順
    • Journal Title

      IFO Research Communications

      Volume: 28 Pages: 109-119

    • Related Report
      2014 Annual Research Report
  • [Journal Article] An organic acid-tolerant HAA1-overexpression mutant of an industrial bioethanol strain of Saccharomyces cerevisiae and its application to the production of bioethanol from sugarcane molasses.2013

    • Author(s)
      Takuya Inaba, Daisuke Watanabe, Yoko Yoshiyama, Koichi Tanaka, Jun Ogawa, Hiroshi Takagi, Hitoshi Shimoi and Jun Shima.
    • Journal Title

      AMB Express

      Volume: 74 Issue: 1 Pages: 1-7

    • DOI

      10.1186/2191-0855-3-74

    • NAID

      120006537405

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of an acetate-tolerant strain of Saccharomyces cerevisiae and characterization by gene expression analysis2012

    • Author(s)
      Haitani, Y., Tanaka, K., Yamamoto, M., Nakamura, T., Ando, A., Ogawa, J. and Shima, J.
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 114(6) Issue: 6 Pages: 648-651

    • DOI

      10.1016/j.jbiosc.2012.07.002

    • NAID

      110009578160

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Overexpression of vacuolar H+-ATPase-related genes in bottom-fermenting yeast enhances ethanol tolerance and fermentation rates during high-gravity fermentation2012

    • Author(s)
      Hasegawa S, Ogata T, Tanaka K, Ando A, Takagai H, Shima J
    • Journal Title

      Journal of the Institute of Brewing

      Volume: 118 Issue: 2 Pages: 179-185

    • DOI

      10.1002/jib.32

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Overexpression of HAA1 gene encoding transcriptional activator enhances acetic acid tolerance in Saccharomyces cerevisiae.2012

    • Author(s)
      Koichi Tanaka, Yukari Ishii, Jun Ogawa and Jun Shima
    • Journal Title

      Appl. Environ. Microbiol.

      Volume: 78 Issue: 22 Pages: 8161-8163

    • DOI

      10.1128/aem.02356-12

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] An organic acid-tolerant HAA1-overexpression strain from industrial bioethanol yeast.

    • Author(s)
      Takuya Inaba, Daisuke Watanabe, Yoko Yoshiyama, Koichi Tanaka, Jun Ogawa, Hiroshi Takagi, Hitoshi Shimoi and Jun Shima.
    • Organizer
      International Conference on Agricultural, Ecological and Medical Sciences (AEMS-2014)
    • Place of Presentation
      Bali (Indonesia)
    • Related Report
      2013 Research-status Report
  • [Presentation] HAA1遺伝子過剰発現酵母株を用いたバイオエタノール生産

    • Author(s)
      稲葉拓哉、渡邊大輔、吉山洋子、田中晃一、小川順、高木博史、下飯 仁、島純
    • Organizer
      シンポジウム「微生物科学研究の多様性と新展開」
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Presentation] 出芽酵母の酢酸耐性はHaa1依存的な細胞内グリセロールの蓄積により増強される

    • Author(s)
      田中晃一、小川順、島純
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Presentation] 産業用バイオエタノール酵母由来のHAA1遺伝子過剰発現株と糖蜜を用いたバイオエタノール生産

    • Author(s)
      稲葉拓哉、渡邊大輔、吉山洋子、田中晃一、小川順、高木博史、下飯 仁、島純
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Presentation] 酵母の有機酸およびエタノールストレス耐性には芳香族アミノ酸チロシンが重要である

    • Author(s)
      ○田中 晃一、小松崎 典子、藤原 しのぶ、石井 由香里、日比 慎、小川 順、島 純
    • Organizer
      第4回日本醸造学会若手シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] 液胞プロトンポンプ関連遺伝子の過剰発現によりビール酵母はエタノールストレス耐性を獲得する

    • Author(s)
      長谷川園子、尾形智夫、田中晃一、安藤 聡、小川 順、高木博史、○島 純
    • Organizer
      第4回日本醸造学会若手シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] 出芽酵母のストレス耐性における芳香族アミノ酸の重要性とその応用

    • Author(s)
      ○田中 晃一、小松崎 典子、藤原 しのぶ、石井 由香里、日比 慎、小川 順、島 純
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸
    • Related Report
      2012 Research-status Report
  • [Presentation] 産業プロセスに有用な酢酸ストレス耐性酵母の同定と耐性メカニズムの解析

    • Author(s)
      ○田中 晃一、灰谷 豊、吉山 洋子、山本 まみ、中村 敏英、安藤 聡、小川 順、 島 純
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      仙台
    • Related Report
      2012 Research-status Report
  • [Presentation] 芳香族アミノ酸の代謝調節による酵母の酢酸ストレス耐性化戦略

    • Author(s)
      田中 晃一、小松崎 典子、藤原 しのぶ、石井 由香里、日比 慎、小川 順、○島 純
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      仙台
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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