Milk oligosaccharides-mediated symbiosis and co-evolution between bifidobacteria and humans

• Project/Area Number: 24580119
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Ishikawa Prefectural University
• Principal Investigator: KATAYAMA Takane 石川県立大学, 生物資源環境学部, 寄附講座教授 (70346104)
• Co-Investigator(Kenkyū-buntansha): HIDAKA Masafumi 東北大学, (連合)農学研究科, 助教 (00584848) ; 廣瀬 潤子 滋賀県立大学, 人間文化学部, 准教授 (40381917)
• Co-Investigator(Renkei-kenkyūsha): HIROSE Junko 滋賀県立大学, 人間環境学部, 准教授 (40381917)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ビフィズス菌 / 母乳オリゴ糖 / 共生 / 共進化 / 糖質分解酵素 / グリコシダーゼ

Outline of Final Research Achievements

Breast-fed infant intestines generally have microbiota rich in bifidobacteria, and the population of the bacteria in the ecosystem drastically decreases after weaning. This suggests that human milk has a bifidogenic compound; however, the mechanism underlying it remains elusive. We have reecntly found that infant gut-associated bifidobacteria are equipped with genetic and enzymatic sets dedicated to the utilization of oligosaccharides contained in human milk (human milk oligosaccharides, HMOs). In this study, we aimed to obtain a comprehensive understanding of the HMOs assimilation pathway of bifidobacteria.

Research Products

• [Journal Article] Novel substrate specificities of two lacto-N-biosidases towards β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1. (2015)
  • Author(s): Gotoh A, Katoh T, Sugiyama Y, Kurihara S, Honda Y, Sakurama H, Kambe T, Ashida H, Kitaoka M, Yamamoto K, Katayama T
  • Journal Title: Carbohydr Res Volume: 408 Pages: 18-24
  • DOI: 10.1016/j.carres.2015.03.005
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] α-N-Acetylglucosaminidase from Bifidobacterium bifidum specifically hydrolyzes α-linked N-acetylglucosamine at nonreducing terminus of O-glycan on gastric mucin (2015)
  • Author(s): 芦田久ら
  • Journal Title: Appl Microbiol Biotechnol. Volume: 99 Issue: 9 Pages: 3941-3948
  • DOI: 10.1007/s00253-014-6201-x
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Distinct substrate specificities of three glycoside hydrolase family 42 β-galactosidases from Bifidobacterium longum subsp. infantis ATCC 15697 (2014)
  • Author(s): Viborg AH, Katayama T, Hachem MA, Andersen MC, Nishimoto M, Clausen MH, Urashima T, Svensson B, Kitaoka M.
  • Journal Title: Glycobiology Volume: 24 Issue: 2 Pages: 208-216
  • DOI: 10.1093/glycob/cwt104
  • Peer Reviewed / Open Access
• [Journal Article] Crystal structures of a glycoside hydrolase family 20 lacto-N-biosidase from Bifidobacterium bifidum (2013)
  • Author(s): 伊藤佑、芦田久、伏信進矢ら
  • Journal Title: J. Biol. Chem. Volume: 288 Issue: 17 Pages: 11795-11806
  • DOI: 10.1074/jbc.m112.420109
  • Peer Reviewed
• [Journal Article] Lacto-N-biosidase encoded by a novel gene of Bifidobacterium longum subspecies longum shows unique substrate specificity and requires a designated chaperone for its active expression (2013)
  • Author(s): 櫻間晴子、芦田久ら
  • Journal Title: J. Biol. Chem. Volume: 288 Issue: 35 Pages: 25194-25206
  • DOI: 10.1074/jbc.m113.484733
  • Peer Reviewed
• [Journal Article] Characterization of the cytosolic β-N-acetylglucosaminidase from Bifidobacterium longum subsp. longum (2013)
  • Author(s): Honda Y, Nishimoto M, Katayama T, and Kitaoka M.
  • Journal Title: Journal of Applied Glycoscience Volume: 60 Pages: 141-146
  • NAID: 10031179276
  • Peer Reviewed
• [Journal Article] Bifidobacterial α-galactosidase with unique carbohydrate-binding module specifically acts on blood group B antigen (2013)
  • Author(s): Takura Wakinaka, Masashi Kiyohara, Shin Kurihara, Akiko Hirata, Thida Chaiwangsri, Takayuki Ohnuma, Tamo Fukamizo, Takane Katayama, Hisashi Ashida, and Kenji Yamamoto
  • Journal Title: Glycobiology Volume: 23 Issue: 2 Pages: 232-240
  • DOI: 10.1093/glycob/cws142
  • Peer Reviewed
• [Journal Article] 1,3-1,4-α-l-Fucosynthase That Specifically Introduces Lewis a/x Antigens into Type-1/2 Chains (2012)
  • Author(s): Haruko Sakurama, Shinya Fushinobu, Masafumi Hidaka, Erina Yoshida, Yuji Honda, Hisashi Ashida, Motomitsu Kitaoka, Hidehiko Kumagai, Kenji Yamamoto, and Takane Katayama
  • Journal Title: Journal of Biological Chemistry Volume: 287: Issue: 20 Pages: 16709-16719
  • DOI: 10.1074/jbc.m111.333781
  • Peer Reviewed
• [Presentation] ヒトミルクオリゴ糖の酵素合成に向けた高効率1,2-α-L-フコシンターゼの作出 (2015)
  • Author(s): 杉山 友太、後藤 愛那、本多 裕司、吉田 永吏奈、栗原 新、北岡 本光、山本 憲二、片山 高嶺
  • Organizer: 日本農芸化学会２０１５年度大会
  • Place of Presentation: 岡山
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] ヒトミルクオリゴ糖に作用するビフィズス菌の酵素 ～機能解析と応用～ (2014)
  • Author(s): 片山高嶺
  • Organizer: ２０１４年度日本農芸化学会大会
  • Place of Presentation: 明治大学
• [Presentation] ビフィズス菌と母乳オリゴ糖～共生と共進化～ (2013)
  • Author(s): 片山高嶺
  • Organizer: ４８回植物感染生理談話会
  • Place of Presentation: 粟津温泉法師
  • Invited
• [Remarks] 石川県立大学腸内細菌共生機構学講座
  • URL: http://host-microbe.ishikawa-pu.ac.jp
• [Remarks] 石川県立大学 腸内細菌共生機構学講座
  • URL: http://host-microbe.ishikawa-pu.ac.jp