Development of pro-biotic lactic acid bacteria protecting intestine from oxidation stress

• Project/Area Number: 24580125
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Tokyo University of Agriculture
• Principal Investigator: NIIMURA YOUICHI 東京農業大学, 応用生物科学部, 教授 (00180563)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 乳酸菌 / プロバイオティックス / 過酸化脂質 / 過酸化脂質分解 / 活性酸素防御系 / バイオアッセイ系開発 / Caenorhabditis elegans / 食品乳酸菌 / 過酸化水素分解 / 酸化ストレス防御 / 腸疾患防御 / 過酸化脂質還元酵素 / 過酸化水素還元酵素 / 過酸化脂質還元 / 過酸化水素 / 過酸化物還元酵素 / Mg-catalase / NADH peroxidase

Outline of Final Research Achievements

In order to develop a pro-biotic lactic acid bacteria system protecting intestine from oxidation stress, lactic acid bacteria, Lactobacillus plantarum P2 was isolated from fermented food. While L. plantarum P2 exhibited high scavenging activity for environmental alkyl hydroperoxide, its activity for environmental hydorgen peroxide was not so high. Next, Pediococcus pentosaceus Be1 exhibiting high scavenging activity for environmental hydrogen peroxide was isolated from fermented vegetable. The scavenging activity for environmental peroxide in vivo of the two isolates were demonstrated by bio-assay system established with Caenorhabditis elegans Δfer-15;mev-1.

Research Products

• [Journal Article] Isolation of lactic acid bacteria exhibiting high scavenging activity for environmental hydrogen peroxide from fermented foods and its two scavenging enzymes for hydrogen peroxide (2016)
  • Author(s): Akio Watanabe, Chiaki Kaneko, Yasuhiro Hamada, Kouji Takeda, Shinya Kimata, Takashi Matsumoto, Akira Abe, Naoto Tanaka, Sanae Okada, Masataka Uchino, Junichi Satoh, Junichi Nakagawa, and Youichi Niimura
  • Journal Title: The Journal of General and Applied Microbiology Volume: 62 Issue: 2 Pages: 75-82
  • DOI: 10.2323/jgam.62.75
  • NAID: 130005148733
  • ISSN: 0022-1260, 1349-8037
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Cucumis sativus secretes 4′-ketoriboflavin under iron-deficient conditions. (2016)
  • Author(s): J. Satoh, H. Koshino, K Sekino, S. Ito, R Katsuta, K. Takeda, E. Yoshimura, F Shinmachi, S. Kawasaki, Y. Niimura and T. Nukada.
  • Journal Title: Biosci. Biotech.Biochem. Volume: 80 Issue: 2 Pages: 363-367
  • DOI: 10.1080/09168451.2015.1095070
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] NADH oxidase and alkyl hydroperoxide reductase subunit C (peroxiredoxin) from Amphibacillus xylanus form an oligomeric assembly (2015)
  • Author(s): Toshiaki Arai, Shinya Kimata, Daichi Mochizuki, Keita Hara, Tamotsu Zako, Masafumi Odaka, Masafumi Yohda, Fumio Arisaka, Shuji Kanamaru, Takashi Matsumoto, Shunsuke Yajima, Junichi Sato, Shinji Kawasaki, Youichi Niimura
  • Journal Title: FEBS Open Bio Volume: 5 Issue: 1 Pages: 124-131
  • DOI: 10.1016/j.fob.2015.01.005
  • Peer Reviewed / Open Access
• [Journal Article] NADH oxidase and alkyl hydroperoxide reductase subunit C (peroxiredoxin) from Amphibacillus xylanus form an oligomeric assembly (2015)
  • Author(s): Toshiaki Arai, Shinya Kimata, Daichi Mochizuki, Keita Hara, Tamotsu Zako, Masafumi Odaka, Masafumi Yohda, Fumio Arisaka, Shuji Kanamaru, Takashi Matsumoto, Shunsuke Yajima, Junichi Sato, Shinji Kawasaki, and Youichi Niimura
  • Journal Title: FEBS Open Bio Volume: 5 Pages: 124-131
  • Peer Reviewed / Open Access
• [Journal Article] Adaptive response of Amphibacillus xylanus to normal aerobic and forced oxidative stress conditions (2014)
  • Author(s): Daichi Mochizuki,13 Toshiaki Arai,13 Masazumi Asano,1 Natsuki Sasakura,1 Toshihiro Watanabe,2 Yuh Shiwa,3 Sanae Nakamura,4 Yoko Katano,4 Shun Fujinami,4 Nobuyuki Fujita,4 Akira Abe,5 Junichi Sato,1 Junichi Nakagawa2 and Youichi Niimura1
  • Journal Title: Microbiology Volume: 160 Issue: 2 Pages: 340-352
  • DOI: 10.1099/mic.0.068726-0
  • Peer Reviewed
• [Journal Article] Purification and characterization of oxygen-inducible heme catlase from oxygen-tolerant Bifidobacterium asteroides. (2013)
  • Author(s): 1. Hayashi K, Maekawa I, Tanaka K, Ijyuin S, Suzuki I, Niimura Y, Kawasaki S.
  • Journal Title: Microbiology Volume: 159 Pages: 89-95
  • Peer Reviewed
• [Journal Article] Evolution and diversification of oxygen metabolisms of aerotolerant anaerobes in the order Bacillales and other bacterial taxonomic groups. (2012)
  • Author(s): 2. Mochizuki D, Tanaka N, Ishikawa M, Endo K, Shiwa Y, Fujita N, Sato J, Niimura Y.
  • Journal Title: The Bulletin of BISMiS Volume: 3 Pages: 1-18
  • Peer Reviewed
• [Presentation] 代謝産物として初めて見つかったリボフラビン（ビタミンB2）誘導体 (2016)
  • Author(s): 佐藤純一, 越野広雪, 関野滉太, 金子元優, 伊藤晋作, 勝田亮, 武田晃治, 吉村悦郎, 新町文絵, 川崎信治, 額田恭郎, 新村洋一
  • Organizer: 日本農芸化学会（2016
  • Place of Presentation: 札幌
  • Year and Date: 2016-03-27
• [Presentation] Amphibacillus xylanusの好気代謝における遊離フラビンとSH化合物に関する研究 (2016)
  • Author(s): 木俣真弥, 名須川哲哉, 及川雅人, 新地大輔, 富塚駿, 吉村悦郎, 有賀智子, 阿部晃, 武田晃治, 佐藤純一, 川崎信治, 新村洋
  • Organizer: 日本農芸化学会
  • Place of Presentation: 札幌
  • Year and Date: 2016-03-27
• [Presentation] NADH oxidase -AhpC (Prx) system functional as NADH oxidase and NADH peroxidase < Protein interaction and its physiological role in Amphibacillus xylanus > (2014)
  • Author(s): Daichi Mochizuki, Shinya Kimata,Toshiaki Arai, Keita Hara, Junichi Sato, Shinji Kawasaki, Shuji Kanamaru, Fumio Arisaka , and Youichi Niimura
  • Organizer: 国際フラビンシンポジウム
  • Place of Presentation: タイ国
  • Year and Date: 2014-07-27 – 2014-07-30
• [Presentation] Iron release from iron storage protein by Escherichia coli ferric reductases in the presence or absence of free flavins (2014)
  • Author(s): Junichi Sato , Eisuke Tanaka , Tetsuya Mori , Kouji Takeda , Etsuro Yoshimura , Junichi Nakagawa, Akira Abe, Shinji Kawasaki, and Youichi Niimura
  • Organizer: 国際フラビンシンポジウム
  • Place of Presentation: タイ国
  • Year and Date: 2014-07-27 – 2014-07-30
• [Presentation] 鉄酸化酵素 (2014)
  • Author(s): 佐藤純一
  • Organizer: 日本農芸化学会
  • Place of Presentation: 明治大学
• [Presentation] 過酸化物還元活性を有する乳酸菌の酸素代謝系 (2013)
  • Author(s): 梅澤秀幸 他9名
  • Organizer: 日本農芸化学会
  • Place of Presentation: 仙台