Development of pro-biotic lactic acid bacteria protecting intestine from oxidation stress
Project/Area Number |
24580125
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied microbiology
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Research Institution | Tokyo University of Agriculture |
Principal Investigator |
NIIMURA YOUICHI 東京農業大学, 応用生物科学部, 教授 (00180563)
|
Project Period (FY) |
2012-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 乳酸菌 / プロバイオティックス / 過酸化脂質 / 過酸化脂質分解 / 活性酸素防御系 / バイオアッセイ系開発 / Caenorhabditis elegans / 食品乳酸菌 / 過酸化水素分解 / 酸化ストレス防御 / 腸疾患防御 / 過酸化脂質還元酵素 / 過酸化水素還元酵素 / 過酸化脂質還元 / 過酸化水素 / 過酸化物還元酵素 / Mg-catalase / NADH peroxidase |
Outline of Final Research Achievements |
In order to develop a pro-biotic lactic acid bacteria system protecting intestine from oxidation stress, lactic acid bacteria, Lactobacillus plantarum P2 was isolated from fermented food. While L. plantarum P2 exhibited high scavenging activity for environmental alkyl hydroperoxide, its activity for environmental hydorgen peroxide was not so high. Next, Pediococcus pentosaceus Be1 exhibiting high scavenging activity for environmental hydrogen peroxide was isolated from fermented vegetable. The scavenging activity for environmental peroxide in vivo of the two isolates were demonstrated by bio-assay system established with Caenorhabditis elegans Δfer-15;mev-1.
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Report
(5 results)
Research Products
(13 results)
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[Journal Article] Isolation of lactic acid bacteria exhibiting high scavenging activity for environmental hydrogen peroxide from fermented foods and its two scavenging enzymes for hydrogen peroxide2016
Author(s)
Akio Watanabe, Chiaki Kaneko, Yasuhiro Hamada, Kouji Takeda, Shinya Kimata, Takashi Matsumoto, Akira Abe, Naoto Tanaka, Sanae Okada, Masataka Uchino, Junichi Satoh, Junichi Nakagawa, and Youichi Niimura
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Journal Title
The Journal of General and Applied Microbiology
Volume: 62
Issue: 2
Pages: 75-82
DOI
NAID
ISSN
0022-1260, 1349-8037
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] NADH oxidase and alkyl hydroperoxide reductase subunit C (peroxiredoxin) from Amphibacillus xylanus form an oligomeric assembly2015
Author(s)
Toshiaki Arai, Shinya Kimata, Daichi Mochizuki, Keita Hara, Tamotsu Zako, Masafumi Odaka, Masafumi Yohda, Fumio Arisaka, Shuji Kanamaru, Takashi Matsumoto, Shunsuke Yajima, Junichi Sato, Shinji Kawasaki, Youichi Niimura
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Journal Title
FEBS Open Bio
Volume: 5
Issue: 1
Pages: 124-131
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] NADH oxidase and alkyl hydroperoxide reductase subunit C (peroxiredoxin) from Amphibacillus xylanus form an oligomeric assembly2015
Author(s)
Toshiaki Arai, Shinya Kimata, Daichi Mochizuki, Keita Hara, Tamotsu Zako, Masafumi Odaka, Masafumi Yohda, Fumio Arisaka, Shuji Kanamaru, Takashi Matsumoto, Shunsuke Yajima, Junichi Sato, Shinji Kawasaki, and Youichi Niimura
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Journal Title
FEBS Open Bio
Volume: 5
Pages: 124-131
Related Report
Peer Reviewed / Open Access
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[Journal Article] Adaptive response of Amphibacillus xylanus to normal aerobic and forced oxidative stress conditions2014
Author(s)
Daichi Mochizuki,13 Toshiaki Arai,13 Masazumi Asano,1 Natsuki Sasakura,1 Toshihiro Watanabe,2 Yuh Shiwa,3 Sanae Nakamura,4 Yoko Katano,4 Shun Fujinami,4 Nobuyuki Fujita,4 Akira Abe,5 Junichi Sato,1 Junichi Nakagawa2 and Youichi Niimura1
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Journal Title
Microbiology
Volume: 160
Issue: 2
Pages: 340-352
DOI
Related Report
Peer Reviewed
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[Presentation] 代謝産物として初めて見つかったリボフラビン(ビタミンB2)誘導体2016
Author(s)
佐藤純一, 越野広雪, 関野滉太, 金子元優, 伊藤晋作, 勝田亮, 武田晃治, 吉村悦郎, 新町文絵, 川崎信治, 額田恭郎, 新村洋一
Organizer
日本農芸化学会(2016
Place of Presentation
札幌
Year and Date
2016-03-27
Related Report
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[Presentation] Amphibacillus xylanusの好気代謝における遊離フラビンとSH化合物に関する研究2016
Author(s)
木俣真弥, 名須川哲哉, 及川雅人, 新地大輔, 富塚駿, 吉村悦郎, 有賀智子, 阿部晃, 武田晃治, 佐藤純一, 川崎信治, 新村洋
Organizer
日本農芸化学会
Place of Presentation
札幌
Year and Date
2016-03-27
Related Report
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