Studies on evolution and functional differentiation of diterpene cyclase genes in rice.

• Project/Area Number: 24580155
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Yamagata University
• Principal Investigator: TOYOMASU Tomonobu 山形大学, 農学部, 教授 (60272085)
• Co-Investigator(Renkei-kenkyūsha): OKADA Kazunori 東京大学, 生物生産工学研究センター, 准教授 (20312241) ; KURODA Masaharu 独立行政法人農業・食品産業技術総合研究機構, 中央農業総合研究センター作物開発研究領域, 主任研究員 (30355581) ; NAKAZONO Mikio 名古屋大学, 大学院生命農学研究科, 教授 (70282697)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: イネ / ジテルペン / 生合成 / 酵素 / 機能分化 / 進化 / ファイトアレキシン / ジベレリン / 野生イネ / フィトアレキシン

Outline of Final Research Achievements

Cultivated rice (Oryza sativa) produces not only diterpene phytohormone gibberellins but also labdane-related diterpene phytoalexins, including momilactones, phytocassanes and oryzalexins. Our studies indicated that enzyme genes responsible for early-step biosynthesis of these diterpenoids express according to their biological roles, one is growth regulation and another is defense. Our studies also showed that momilactones and phytocassanes were accumulated in leaf blade samples of wild species Oryza rufipogon.

Research Products

• [Journal Article] Transcripts of two ent-copalyl diphosphate synthase genes differentially localize in rice plants according to their distinct biological roles. (2015)
  • Author(s): Toyomasu, T., Usui, M., Sugawara, C., Kanno, Y., Sakai, A., Takahashi, H., Nakazono, M., Kuroda, M., Miyamoto, K., Morimoto, Y., Mitsuhashi, W., Okada, K., Yamaguchi, S., and Yamane, H.
  • Journal Title: Journal of Experimental Botany Volume: 66 Issue: 1 Pages: 369-376
  • DOI: 10.1093/jxb/eru424
  • Peer Reviewed / Open Access / Acknowledgement Compliant