Biochemical characterization of reductases responsible for the formation of multiple floral scents in Petunia

• Project/Area Number: 24580162
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Yamaguchi University (2013-2014); Kyoto University (2012)
• Principal Investigator: KOEDUKA Takao 山口大学, 農学部, 助教 (30565106)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 植物二次代謝 / 香気成分 / ペチュニア / 還元酵素

Outline of Final Research Achievements

Given the chemical structures of phenylpropanoid volatiles such as dihydroconiferyl acetate, we assumed that the reduction of phenylpropanoids into dihydro derivatives must be catalyzed by double bond reductases in Petunia axillaris subsp. parodii unlike other petunia species. In this study, we investigated the double bond reductases that are responsible for the branching of the biosynthetic pathway of phenylpropanoid volatiles in petunia flowers.

Research Products

• [Journal Article] The phenylpropene synthase pathway and its applications in the engineering of volatile phenylpropanoids in plants (2014)
  • Author(s): Koeduka T
  • Journal Title: Plant Biotechnology Volume: 31 Pages: 401-407
  • NAID: 130004713021
  • Peer Reviewed / Open Access
• [Journal Article] Microbial production of plant specialized metabolites (2014)
  • Author(s): Suzuki S, Koeduka T, Sugiyama A, Yazaki K, Umezawa T
  • Journal Title: Plant Biotechnology Volume: 31 Pages: 465-482
  • NAID: 130004721175
  • Peer Reviewed / Open Access
• [Journal Article] Biosynthesis of geraniol, a potent aroma compound in giger rhizome (Zingiber officinale): Molecular cloning and characterization of geraniol dehydrogenase (2014)
  • Author(s): Iijima Y, Koeduka T, Suzuki H, Kubota K
  • Journal Title: Plant Biotechnology Volume: 31 Pages: 525-534
  • Peer Reviewed / Open Access
• [Journal Article] Metabolic engineering of flavonoids with prenyltransferase and chalcone isomerase genes in tomato fruits (2014)
  • Author(s): Kawasaki T, Koeduka T Sugiyama A, Sasaki K, Linley PJ, Shitan N, Kumano T, Yamamoto H, Ezura H, Kuzuyama T, Yazaki K
  • Journal Title: Plant Biotechnology Volume: 31 Pages: 567-571
  • NAID: 130004713024
  • Peer Reviewed / Open Access
• [Journal Article] Bioactivity of natural O-prenylated phenylpropenes from Illicium anisatum leaves and their derivatives against spider mites and fungal pathogens. (2014)
  • Author(s): Koeduka T, Sugimoto K, Watanabe B, Someya N, Kawanishi D, Gotoh T, Ozawa R, Takabayashi J, Matsui K, Hiratake J.
  • Journal Title: Plant Biology Volume: 16 Pages: 451-456
  • Peer Reviewed
• [Journal Article] A coumarin-specific prenyltransferase catalyzes the crucial biosynthetic reaction for furanocoumarin formation in parsley. (2014)
  • Author(s): Karamat F, Olry A, Munakata R, Koeduka T, Sugiyama A, Paris C, Hehn A, Bourgaud F, Yazaki K.
  • Journal Title: The Plant Journal Volume: 77 Pages: 627-638
  • Peer Reviewed
• [Journal Article] Enhancement of production of eugenol and its glycosides in transgenic aspen plants via genetic engineering. (2013)
  • Author(s): Koeduka T, Suzuki S, Iijima Y, Ohnishi T, Suzuki H, Watanabe B, Shibata D, Umezawa T, Pichersky E, Hiratake J.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 436 Pages: 73-78
  • NAID: 120005296113
  • Peer Reviewed
• [Presentation] サンショウ葉の香気成分とセスキテルペン合成酵素に関する研究 (2015)
  • Author(s): 藤田芳勧, 飯島陽子, 鈴木秀幸, 松井健二, 肥塚崇男
  • Organizer: 日本農芸化学会中四国支部第41回講演会
  • Place of Presentation: 水産大学校（山口県下関市）
  • Year and Date: 2015-01-24
• [Presentation] 苔類ゼニゴケにおけるオキシリピン代謝酵素CYP74ファミリー酵素遺伝子の機能解析 (2014)
  • Author(s): 肥塚崇男、石崎公庸、河内孝之、松井健二
  • Organizer: 第55回植物生理学会
  • Place of Presentation: 富山大学（富山市）
• [Presentation] フラノクマリン生合成初発反応を担うクマリン特異的プレニル基転移酵素の解析 (2014)
  • Author(s): 棟方涼介、Karamat Fazeelat, Alexandre Olry,肥塚崇男、杉山暁史、Cedric Paris, Alain Hehn, Frederic Bourgaud,矢崎一史
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 明治大学（川崎市）
• [Presentation] Specialized metabolites and biochemical characterization of double bond reductase in Marchantia polymorpha L. (2012)
  • Author(s): Takao Koeduka, Jun Hiratake, Kenji Matsui
  • Organizer: Marchantia Workshop 2012
  • Place of Presentation: 熊本県阿蘇郡南阿蘇村 ホテルグリーンピア南阿蘇
  • Invited