Analysis of taste-recognition mechanism through taste receptor
| Project/Area Number |
24580172
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Nigata University of Phermacy and Applied Life Sciences (2014)
Yamagata University (2012-2013) |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | 甘味受容体 |
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| Outline of Final Research Achievements |
Sweet taste is perceived through sweet-taste receptor, which is a membrane protein of taste cells. In this study, we tried to construct expression system of amino terminal domain (ATD) of human sweet-taste receptor, that is a heterodimer of hT1R2 and hT1R3, in order to analyze the interaction between sweet-taste receptor and sweeteners.
We succeeded to produce ATD of hT1R2 and hT1R3 fused with maltose-binding protein (MBP) in E.coli. We further analyzed hT1R3-ATD, since its expression level was higher than that of hT1R2-ATD. The protein was very unstable, and the molecular size was found to be much larger than the size expected.
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Report
(4 results)
Research Products
(1 results)
