Analysis of taste-recognition mechanism through taste receptor

Research Project

Project/Area Number	24580172
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Food science
Research Institution	Nigata University of Phermacy and Applied Life Sciences (2014) Yamagata University (2012-2013)
Principal Investigator	SHIMIZU-IBUKA Akiko 新潟薬科大学, 応用生物科学部, 教授 (60301420)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000) Fiscal Year 2014: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000) Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Keywords	甘味受容体
Outline of Final Research Achievements	Sweet taste is perceived through sweet-taste receptor, which is a membrane protein of taste cells. In this study, we tried to construct expression system of amino terminal domain (ATD) of human sweet-taste receptor, that is a heterodimer of hT1R2 and hT1R3, in order to analyze the interaction between sweet-taste receptor and sweeteners. We succeeded to produce ATD of hT1R2 and hT1R3 fused with maltose-binding protein (MBP) in E.coli. We further analyzed hT1R3-ATD, since its expression level was higher than that of hT1R2-ATD. The protein was very unstable, and the molecular size was found to be much larger than the size expected.

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(4 results)