| Project/Area Number |
24580205
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Shinshu University (2015)
Kobe Gakuin University (2012-2014) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
IRINO Yasuhiro 神戸大学, 医学研究科, 助教 (10415565)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 健康機能性食品 / DNA合成酵素 / 酵素阻害剤 / 抗炎症作用 / 抗がん作用 / マイクロアレイ解析 / ノックダウン解析 / 作用機序 / β-マンゴスチン / ステロール配糖体エステル / 抗炎症活性 / 抗アレルギー活性 / クルクミン / ナフトキノン / メタボローム解析 / RNA干渉 / 米糠 / フェルラ酸シクロアルテニル / 化学的ノックアウト解析 / 食品成分 / 栄養素 / 抗がん活性 |
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| Outline of Final Research Achievements |
Some nutrients and food components are known to have various healthy functions. In this study, I established the in vitro screening method of mammalian DNA polymerase (pol) inhibitors, and screened them from nutrients and food components. Especially, I found a cerebroside (glucosyl ceramide, N-{1-[(β-D-Glucopyranosyloxy)methyl]-2-hydroxyheptadeca-3,7-dien-1-yl}-2-hydroxyhexadecanamide) and a steroidal glycoside (eleutheroside A) as mammalian pol lambda specific inhibitors from soybean (Glycine max L.), and beta-mangostin as a DNA replicative pols inhibitor from mangosteen (Garcinia mangostana L.). These inhibitors of pol lambda and DNA replicative pols were related to anti-inflammatory effect and anticancer effect, respectively.
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