Revealing the mechanism of ring expansion reaction to tropolon formation uniquely found in woody plant.
| Project/Area Number |
24580248
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Wood science
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| Research Institution | Kyushu University |
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Principal Investigator |
FUJITA Koki 九州大学, (連合)農学研究科(研究院), 助教 (90264100)
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| Co-Investigator(Kenkyū-buntansha) |
TSUTSUMI Yuji 九州大学, 大学院農学研究院, 教授 (30236921)
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| Co-Investigator(Renkei-kenkyūsha) |
ICHINOSE Hirofumi 九州大学, 大学院農学研究院, 准教授 (00432948)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | ヒノキチオール / トロポロン環 / テルピノレン / ＧＣＭＳ / Ｐ４５０ / ジオキシゲナーゼ / テルペン / Cupressus lusitanica / トロポロン / P450 / メキシコイトスギ / 重水素ラベル / エポキシ / 生合成 / モノテルペン |
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| Outline of Final Research Achievements |
Feeding experiment with deuterized terpinolene into Cupressus lusitanica cultured cell revealed that terpinolene is the first olefin monoterpene intermediate to biosynthesize beta-thujaplicin. This pathway was the first report that tropolon ring was formed from terpenoid.
Serching other intermediates by comparing GCMS analysis of extractives from normal and deuterized terpinolene fed to the cell did not give the good candidate of the intermediates. Therefore, we should consider another pathway driven by dioxygenase.
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Report
(4 results)
Research Products
(8 results)
