Production of recombinant salmon insulin-like growth factor binding proteins

• Project/Area Number: 24580256
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General fisheries
• Research Institution: Hokkaido University
• Principal Investigator: SHIMIZU Munetaka 北海道大学, 水産科学研究科(研究院), 講師 (90431337)
• Co-Investigator(Renkei-kenkyūsha): ANDO Hironori 新潟大学, 自然科学系, 教授 (60221743)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 成長 / サケ科魚類 / ホルモン / 組換え蛋白 / インスリン様成長因子 / 結合蛋白 / 脳下垂体 / 細胞培養 / 成長ホルモン

Outline of Final Research Achievements

The present study dealt with production of functional recombinant insulin-like growth factor binding proteins (IGFBPs) that are important regulators of IGFs. We first established a primary pituitary cell culture for immature masu salmon. We next expressed recombinant IGFBP-1a and -1b using bacterial cell system. These recombinant IGFBPs were added to the pituitary cell culture to look at how the inhibitory action of IGF-I on GH release was modulated. The result suggests that although salmon IGFBP-1a and -1b were believed to be inhibitors of IGF-I actions, they actually enhanced the action of IGF-I on the pituitary. Availability of recombinant protein will be extremely useful to further analyze functions of fish IGFBPs.

Research Products

• [Journal Article] Development of a time-resolved fluoroimmunoassay for salmon insulin-like growth factor binding protein-1b (2015)
  • Author(s): Fukuda, M., Kaneko, N., Kawaguchi, K., Hevroy, E.M., Hara, A., and Shimizu, M.
  • Journal Title: Comparative Biochemistry and Physiology Part A Volume: in press
  • NAID: 120005828484
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Responses of insulin-like growth factor (IGF)-I and two IGF-binding protein-1 subtypes to fasting and re-feeding, and relationship with individual growth rate in yearling masu salmon (Oncorhynchus masou) (2013)
  • Author(s): Kawaguchi, K., Kaneko, N., Fukuda, M., Nakano, Y., Kimura, S., Hara, A., and Shimizu, M.
  • Journal Title: Comparative Biochemistry and Physiology, Part A Volume: 165A Issue: 2 Pages: 191-198
  • DOI: 10.1016/j.cbpa.2013.02.029
  • Peer Reviewed
• [Journal Article] GH-IGF system regulation of attenuated muscle growth and lipolysis in Atlantic salmon reared at elevated sea temperatures (2013)
  • Author(s): Hevroy, E.M., Hunskar C., Gelder, S., Shimizu, M., Waagbo, R., Breck, O., Takle, H., Sussort, S., and Hansen, T.
  • Journal Title: Journal of Comparative Physiology B Volume: 183 Issue: 2 Pages: 243-259
  • DOI: 10.1007/s00360-012-0704-5
  • Peer Reviewed
• [Journal Article] Responses of insulin-like growth factor (IGF)-I and two IGF-binding protein-1 subtypes to fasting and re-feeding, and relationships with individual growth rate in yearling masu salmon (Oncorhynchus masou) (2013)
  • Author(s): Kawaguchi, K., Kaneko, N., Fukuda, M., Nakano, Y., Kimura, S., Hara, A., and Shimizu, M.
  • Journal Title: Comparative Biochemistry and Physiology, Part A Volume: 165A Pages: 191-198
  • Peer Reviewed
• [Presentation] Two insulin-like growth factor binding protein (IGFBP)-1 subtypes as negative growth indices in salmon (2014)
  • Author(s): Munetaka Shimizu, Kohei Kawaguchi, Miki Fukuda, Nobuto Kaneko, and Yusuke Nakano
  • Organizer: 11th International Congress on the Biology of Fish
  • Place of Presentation: Heriot-Watt University, Edinburgh, UK
  • Year and Date: 2014-08-06
• [Presentation] サケ科魚類のインスリン様成長因子結合蛋白の免疫測定法の確立と成長との関係 (2013)
  • Author(s): 福田美樹･川口航平･木村志津雄･原 彰彦・清水宗敬
  • Organizer: 第7回サケ学研究会
  • Place of Presentation: 北海道大学（北海道札幌市）
• [Presentation] サクラマスの2種類のインスリン様成長因子結合蛋白-1の絶食･再給餌に対する反応と成長率との関係 (2013)
  • Author(s): 川口航平･福田美樹･中野裕介･木村志津雄･原 彰彦・清水宗敬
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学（東京都品川区）
• [Presentation] Circulating salmon insulin-like growth factor binding proteins: Duplication matters (2012)
  • Author(s): Shimizu, M., Suzuki, S., Kishimoto, K., Yamaguchi, T., Hara, A., and Dickhoff, W.W.
  • Organizer: 7th International Symposium on Fish Endocrinology
  • Place of Presentation: NH City & Tower Hotel (Argentina)
• [Book] Insulin-like growth factor binding proteins (in Handbook of Hormones: Comparative and General Endocrinology) (2015)
  • Author(s): Shimizu, M.
  • Total Pages: 3
  • Publisher: Elsevier
• [Book] Insulin-like growth factor-I (in Handbook of Hormones: Comparative and General Endocrinology) (2015)
  • Author(s): Shimizu, M.
  • Total Pages: 3
  • Publisher: Elsevier
• [Book] Insulin-like growth factor-II (in Handbook of Hormones: Comparative and General Endocrinology) (2015)
  • Author(s): Shimizu, M.
  • Total Pages: 3
  • Publisher: Elsevier
• [Book] サケ学大全 (2013)
  • Author(s): 帰山雅秀、永田光博、中川大介
  • Total Pages: 296
  • Publisher: 北海道大学出版会
• [Remarks] Munetaka Shimizu, Ph.D.
  • URL: http://www.geocities.jp/fishvitellogenin/ms/Welcome.html
• [Remarks] 研究者が作成した研究内容に関するWebページ
  • URL: http://www.geocities.jp/fishvitellogenin/ms/Welcome.html
• [Remarks] 研究者が作成した研究内容に関するWebページ
  • URL: http://www.geocities.jp/fishvitellogenin/ms/Welcome.html