Development of effective transformation methods for harmful algae

• Project/Area Number: 24580268
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General fisheries
• Research Institution: Kochi University
• Principal Investigator: ADACHI Masao 高知大学, 自然科学系, 教授 (70274363)
• Co-Investigator(Kenkyū-buntansha): OHNISHI Kohei 高知大学, 教育研究部総合科学系, 教授 (50211800)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 有害・有毒プランクトン / 形質転換 / 有害・有毒藻 / ウイルスプロモーター

Outline of Final Research Achievements

The objective for this study is to develop a transformation system of marine harmful microalgae using new promoters from microalgae-infecting viruses. We have determined the genome sequences of viruses which infect the diatom Rhizosolenia, the causative organism for bleaching of aquacultured ‘nori’ (Porphyra yezoensis) and the fish-killing raphidophyte Heterosigma akashiwo. We have succeeded in cultivation of the two harmful algae on semi-solid media containing low concentration of agarose. We have established a transformation method of Rhizosolenia using a vector which contained a new viral promoter isolated from a virus infecting the diatom. This is the first study describing the transformation system of harmful microalgae.

Research Products

• [Journal Article] Development of a biofuel production system using genetic engineered marine diatoms (2014)
  • Author(s): Masao Adachi, Shigeru Okada, Shigeki Sawayama, Kengo Suzuki
  • Journal Title: Journal of Japan Institute of Energy Volume: 93 Pages: 444-451
  • NAID: 110009818961
• [Journal Article] Effects of temperature, salinity and their interaction on growth of toxic Ostreopsis sp. 1 and Ostreopsis sp. 6 (Dinophyceae) isolated from Japanese coastal waters (2013)
  • Author(s): Yuko Tanimoto, Haruo Yamaguchi, Takamichi Yoshimatsu, Shinya Sato, Masao Adachi
  • Journal Title: Fisheries Sci. Volume: 79 Issue: 2 Pages: 285-291
  • DOI: 10.1007/s12562-013-0597-6
  • NAID: 10031138666
  • Peer Reviewed
• [Journal Article] Quantitative PCR method for enumeration of cells of cryptic species of the toxic marine dinoflagellate Ostreopsis spp. in coastal waters of Japan. (2013)
  • Author(s): Naohito Hariganeya, Yuko Tanimoto, Haruo Yamaguchi, Tomohiro Nishimura, Wittaya Tawong, Hiroshi Sakanari, Takamichi Yoshimatsu, Shinya Sato, Christina M. Preston, Masao Adachi
  • Journal Title: PLoS ONE Volume: 8 Issue: 3 Pages: e57627-e57627
  • DOI: 10.1371/journal.pone.0057627
  • Peer Reviewed
• [Journal Article] Genetic diversity and distribution of the ciguatera-causing dinoflagellate Gambierdiscus spp. (Dinophyceae) in coastal areas of Japan (2013)
  • Author(s): Tomohiro Nishimura, Shinya Sato, Wittaya Tawong, Hiroshi Sakanari, Keita Uehara, Md Mahfuzur Rahman Shah, Shoichiro Suda, Takeshi Yasumoto, Yohsuke Taira, Haruo Yamaguchi, Masao Adachi
  • Journal Title: PLoS ONE Volume: 8 Issue: 4 Pages: e60882-e60882
  • DOI: 10.1371/journal.pone.0060882
  • Peer Reviewed
• [Journal Article] LC-MS/MS analysis of palytoxin analogues in blue humphead parrotfish Scarus ovifrons causing human poisoning in Japan. (2013)
  • Author(s): Toshiyuki Suzuki, Ryuichi Watanabe, Ryoji Matsushima, Kenji Ishihara, Hajime Uchida, Saori Kikutsugi, Tomoko Harada , Hiroshi Nagai, Masao Adachi, Takeshi Yasumoto, Masakazu Murata
  • Journal Title: Food Additives & Contaminants Volume: 30 Issue: 8 Pages: 1358-1364
  • DOI: 10.1080/19440049.2013.790085
  • Peer Reviewed
• [Journal Article] Culture method and growth characteristics of the marine benthic dinoflagellate Ostreopsis spp. isolated from Japanese coastal waters. (2012)
  • Author(s): Haruo Yamaguchi, Yuko Tanimoto, Takamichi Yoshimatsu, Shinya Sato, Tomohiro Nishimura, Keita Uehara, Masao Adachi
  • Journal Title: Fisheries Science Volume: 78 Issue: 5 Pages: 993-1000
  • DOI: 10.1007/s12562-012-0530-4
  • NAID: 10030871684
  • Peer Reviewed
• [Journal Article] Effects of temperature, salinity and their interaction on growth of the benthic dinoflagellate Ostreopsis cf. ovata (Dinophyceae) from Japanese coastal waters. (2012)
  • Author(s): Haruo Yamaguchi, Takamichi Yoshimatsu, Yuko Tanimoto, Shinya Sato, Tomohiro Nishimura, Keita Uehara, Masao Adachi
  • Journal Title: Phycological Research Volume: 60 Issue: 4 Pages: 297-304
  • DOI: 10.1111/j.1440-1835.2012.00660.x
  • NAID: 10031136645
  • Peer Reviewed
• [Journal Article] LC-MS/MS analysis of novel ovatoxin isomers in several Ostreopsis strains collected in Japan. (2012)
  • Author(s): Toshiyuki Suzuki, Ryuichi Watanabe, Hajime Uchida, Ryoji Matsushima, Hiroshi Nagai, Takeshi Yasumoto, Takamichi Yoshimatsu, Shinya Sato, Masao Adachi
  • Journal Title: Harmful Algae Volume: 20 Pages: 81-91
  • Peer Reviewed
• [Presentation] 珪藻に感染するウイルス由来プロモーターの活性評価 (2014)
  • Author(s): 角野貴志・山口亜利沙・外丸裕司・長崎慶三・岡見卓馬・吉良望・福永一成・山口晴生・大西浩平・足立真佐雄
  • Organizer: 第1回分子珪藻研究会
  • Place of Presentation: 関西学院大学梅田キャンパス
  • Year and Date: 2014-12-15
• [Presentation] 珪藻に感染するウイルス由来プロモーターの活性評価 (2014)
  • Author(s): 角野貴志・山口亜利沙・外丸裕司・長崎慶三・岡見卓馬・吉良望・福永一成・山口晴生・大西浩平・足立真佐雄
  • Organizer: 平成26年度日本水産学会春季大会
  • Place of Presentation: 北海道大学
• [Presentation] 海洋細菌のフォスフォトリエステラーゼ産生能 (2014)
  • Author(s): 山口晴生・有坂大志・関美樹・外丸裕司・足立真佐雄
  • Organizer: 平成26年度日本水産学会春季大会
  • Place of Presentation: 北海道大学
• [Presentation] Algal Viral Promoter Useful for Marine Diatom Transformation. (2013)
  • Author(s): Masao Adachi, Takashi Kadono, Arisa Miyagawa-Yamaguchi, Yuji Tomaru, Keizo Nagasaki, Takuma Okami, Nozomu Kira, Kazunari Fukunaga, Haruo Yamaguchi and Kohei Ohnishi
  • Organizer: EMBO Workshop, The Molecular Life of Diatoms
  • Place of Presentation: Paris, France
• [Presentation] 珪藻に感染するウイルス由来プロモーターの活性評価 (2013)
  • Author(s): 角野貴志・山口亜利沙・外丸裕司・長崎慶三・岡見卓馬・吉良望・福永一成・山口晴生・大西浩平・足立真佐雄
  • Organizer: 珪藻に感染するウイルス由来プロモーターの活性評価
  • Place of Presentation: 沖縄県市長村自治会館
• [Presentation] 海産珪藻の炭化水素生合成に関わる酵素群のRNA-Seqによる遺伝子発現解析 (2013)
  • Author(s): 福永一成・吉松孝倫・大西裕美・角野貴志・山口晴生・足立真佐雄
  • Organizer: 第15回マリンバイオテクノロジー学会大会
  • Place of Presentation: 沖縄県市長村自治会館
• [Presentation] 土佐湾沿岸域における付着性渦鞭毛藻Gambierdiscus属の動態 (2013)
  • Author(s): 西村朋宏･坂成浩嗣･Wittaya Tawong･上原啓太・井口大輝・池上拓志・中村正利・吉岡拓也・阿部翔太・山口晴生･足立真佐雄
  • Organizer: 2013年日本ベントス学会・日本プランクトン学会合同大会
  • Place of Presentation: 東北大学
• [Presentation] 海産珪藻Rhizosolenia setigeraの増殖に及ぼす水温、塩分および光強度の影響 (2013)
  • Author(s): 吉松孝倫・田中愛依・谷知宏・山口晴生・足立真佐
  • Organizer: 平成25年度日本水産学会秋季大会
  • Place of Presentation: 三重大学
• [Presentation] 海産珪藻Phaeodactylum tricornutum の培養法ならびに増殖生理 (2013)
  • Author(s): 吉松孝倫・田中愛依・谷知宏・山口晴生・岡内正典・足立真佐雄
  • Organizer: 平成25年度日本水産学会秋季大会
  • Place of Presentation: 三重大学
• [Presentation] 海産珪藻Chaetoceros属藻が溶存核酸からリン・窒素を多段的に獲得する可能性について (2013)
  • Author(s): 小林崇晃・山口晴生・足立真佐雄
  • Organizer: 平成25年度日本水産学会秋季大会
  • Place of Presentation: 三重大学
• [Presentation] 中心目珪藻Rhizosolenia setigeraの炭化水素及び脂質生産と培地条件の検討 (2013)
  • Author(s): 石井健一郎・大西晃・足立真佐雄・澤山茂樹
  • Organizer: 平成25年度日本水産学会秋季大会
  • Place of Presentation: 三重大学
• [Presentation] 15．付着性有毒渦鞭毛藻類 (2013)
  • Author(s): 足立真佐雄
  • Organizer: 平成25年度水産環境保全委員会研究会「有害有毒プランクトンの分類・生理・生態・生活史・個体群動態」
  • Place of Presentation: 三重大学
• [Presentation] Algal Viral Promoter Useful for Marine Diatom Transformation. (2012)
  • Author(s): Masao Adachi, Arisa Miyagawa-Yamaguchi, Yuji Tomaru, Keizo Nagasaki, Kazunari Fukunaga, Kohei Ohno, Takuma Okami, Nozomu Kira, Kohei Ohnishi and Haruo Yamaguchi
  • Organizer: 2nd International conference on Algal Biomass, Biofuels and Bioproducts
  • Place of Presentation: Westin San Diego, USA
• [Presentation] Effect of temperature change on the dominant species of Gambierdiscus in Japan - From a non-toxic species to a toxic species? (2012)
  • Author(s): Masao Adachi, Takamichi Yoshimatsu, Haruka Iwamoto, Tomohiro Nishimura and Haruo Yamaguchi
  • Organizer: PICES 2012 Annual Meeting
  • Place of Presentation: Hiroshima, Japan
  • Invited
• [Presentation] Transformation of the diatom Rhizosolenia setigera using diatom infecting viral promoters. (2012)
  • Author(s): Kazunari Fukunaga, Naoto Hariganeya, Haruo Yamaguchi, Yuji Tomaru, Keizo Nagasaki, Masao Adachi
  • Organizer: The 9th APMBC2012
  • Place of Presentation: Kochi, Japan
• [Presentation] Effect of temperature change on the dominant species of Gambierdiscus in Japan - from a non-toxic species to a toxic species (2012)
  • Author(s): Masao Adachi, Takamichi Yoshimatsu, Haruka Iwamoto, Tomohiro Nishimura and Haruo Yamaguchi
  • Organizer: 15th ICHA 2012 KOREA
  • Place of Presentation: Gyeongnam, Korea
• [Presentation] Development of a quantitative PCR assay for the detection and enumeration of the ciguatera-causing dinoflagellate Gambierdiscus spp. in Japanese coastal areas. (2012)
  • Author(s): Tomohiro Nishimura, Naohito Hariganeya, Tawong Wittaya, Hiroshi Sakanari, Yuko Tanimoto, Haruo Yamaguchi and Masao Adachi
  • Organizer: 15th ICHA 2012 KOREA
  • Place of Presentation: Gyeongnam, Korea
• [Presentation] Effects of temperature and salinity on growth of toxic dinoflagellate Ostreopsis spp. isolated from Japanese coastal area. (2012)
  • Author(s): Yuko Tanimoto, Haruo Yamaguchi, Takamichi Yoshimatsu and Masao Adachi
  • Organizer: 15th ICHA 2012 KOREA
  • Place of Presentation: Gyeongnam, Korea
• [Presentation] The phylogeny of the dinoflagellate Ostreopsis spp. from Thailand. (2012)
  • Author(s): Wittaya Tawong, Tomohiro Nishimura, Hiroshi Sakanari, Haruo Yamaguchi and Masao Adachi
  • Organizer: 15th ICHA 2012 KOREA
  • Place of Presentation: Gyeongnam, Korea
• [Presentation] Clarification of growth-irradiance relationships in the toxic Ostreopsis species cultures using the newly devised method. (2012)
  • Author(s): Haruo Yamaguchia, Yuya Tomoria, Yuko Tanimotoa, Masao Adachi
  • Organizer: 15th ICHA 2012 KOREA
  • Place of Presentation: Gyeongnam, Korea