• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of effective transformation methods for harmful algae

Research Project

Project/Area Number 24580268
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General fisheries
Research InstitutionKochi University

Principal Investigator

ADACHI Masao  高知大学, 自然科学系, 教授 (70274363)

Co-Investigator(Kenkyū-buntansha) OHNISHI Kohei  高知大学, 教育研究部総合科学系, 教授 (50211800)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords有害・有毒プランクトン / 形質転換 / 有害・有毒藻 / ウイルスプロモーター
Outline of Final Research Achievements

The objective for this study is to develop a transformation system of marine harmful microalgae using new promoters from microalgae-infecting viruses. We have determined the genome sequences of viruses which infect the diatom Rhizosolenia, the causative organism for bleaching of aquacultured ‘nori’ (Porphyra yezoensis) and the fish-killing raphidophyte Heterosigma akashiwo. We have succeeded in cultivation of the two harmful algae on semi-solid media containing low concentration of agarose. We have established a transformation method of Rhizosolenia using a vector which contained a new viral promoter isolated from a virus infecting the diatom. This is the first study describing the transformation system of harmful microalgae.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (28 results)

All 2014 2013 2012

All Journal Article (8 results) (of which Peer Reviewed: 7 results) Presentation (20 results) (of which Invited: 1 results)

  • [Journal Article] Development of a biofuel production system using genetic engineered marine diatoms2014

    • Author(s)
      Masao Adachi, Shigeru Okada, Shigeki Sawayama, Kengo Suzuki
    • Journal Title

      Journal of Japan Institute of Energy

      Volume: 93 Pages: 444-451

    • NAID

      110009818961

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Effects of temperature, salinity and their interaction on growth of toxic Ostreopsis sp. 1 and Ostreopsis sp. 6 (Dinophyceae) isolated from Japanese coastal waters2013

    • Author(s)
      Yuko Tanimoto, Haruo Yamaguchi, Takamichi Yoshimatsu, Shinya Sato, Masao Adachi
    • Journal Title

      Fisheries Sci.

      Volume: 79 Issue: 2 Pages: 285-291

    • DOI

      10.1007/s12562-013-0597-6

    • NAID

      10031138666

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Quantitative PCR method for enumeration of cells of cryptic species of the toxic marine dinoflagellate Ostreopsis spp. in coastal waters of Japan.2013

    • Author(s)
      Naohito Hariganeya, Yuko Tanimoto, Haruo Yamaguchi, Tomohiro Nishimura, Wittaya Tawong, Hiroshi Sakanari, Takamichi Yoshimatsu, Shinya Sato, Christina M. Preston, Masao Adachi
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 3 Pages: e57627-e57627

    • DOI

      10.1371/journal.pone.0057627

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Genetic diversity and distribution of the ciguatera-causing dinoflagellate Gambierdiscus spp. (Dinophyceae) in coastal areas of Japan2013

    • Author(s)
      Tomohiro Nishimura, Shinya Sato, Wittaya Tawong, Hiroshi Sakanari, Keita Uehara, Md Mahfuzur Rahman Shah, Shoichiro Suda, Takeshi Yasumoto, Yohsuke Taira, Haruo Yamaguchi, Masao Adachi
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 4 Pages: e60882-e60882

    • DOI

      10.1371/journal.pone.0060882

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] LC-MS/MS analysis of palytoxin analogues in blue humphead parrotfish Scarus ovifrons causing human poisoning in Japan.2013

    • Author(s)
      Toshiyuki Suzuki, Ryuichi Watanabe, Ryoji Matsushima, Kenji Ishihara, Hajime Uchida, Saori Kikutsugi, Tomoko Harada , Hiroshi Nagai, Masao Adachi, Takeshi Yasumoto, Masakazu Murata
    • Journal Title

      Food Additives & Contaminants

      Volume: 30 Issue: 8 Pages: 1358-1364

    • DOI

      10.1080/19440049.2013.790085

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Culture method and growth characteristics of the marine benthic dinoflagellate Ostreopsis spp. isolated from Japanese coastal waters.2012

    • Author(s)
      Haruo Yamaguchi, Yuko Tanimoto, Takamichi Yoshimatsu, Shinya Sato, Tomohiro Nishimura, Keita Uehara, Masao Adachi
    • Journal Title

      Fisheries Science

      Volume: 78 Issue: 5 Pages: 993-1000

    • DOI

      10.1007/s12562-012-0530-4

    • NAID

      10030871684

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effects of temperature, salinity and their interaction on growth of the benthic dinoflagellate Ostreopsis cf. ovata (Dinophyceae) from Japanese coastal waters.2012

    • Author(s)
      Haruo Yamaguchi, Takamichi Yoshimatsu, Yuko Tanimoto, Shinya Sato, Tomohiro Nishimura, Keita Uehara, Masao Adachi
    • Journal Title

      Phycological Research

      Volume: 60 Issue: 4 Pages: 297-304

    • DOI

      10.1111/j.1440-1835.2012.00660.x

    • NAID

      10031136645

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] LC-MS/MS analysis of novel ovatoxin isomers in several Ostreopsis strains collected in Japan.2012

    • Author(s)
      Toshiyuki Suzuki, Ryuichi Watanabe, Hajime Uchida, Ryoji Matsushima, Hiroshi Nagai, Takeshi Yasumoto, Takamichi Yoshimatsu, Shinya Sato, Masao Adachi
    • Journal Title

      Harmful Algae

      Volume: 20 Pages: 81-91

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 珪藻に感染するウイルス由来プロモーターの活性評価2014

    • Author(s)
      角野貴志・山口亜利沙・外丸裕司・長崎慶三・岡見卓馬・吉良望・福永一成・山口晴生・大西浩平・足立真佐雄
    • Organizer
      第1回分子珪藻研究会
    • Place of Presentation
      関西学院大学梅田キャンパス
    • Year and Date
      2014-12-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] 珪藻に感染するウイルス由来プロモーターの活性評価2014

    • Author(s)
      角野貴志・山口亜利沙・外丸裕司・長崎慶三・岡見卓馬・吉良望・福永一成・山口晴生・大西浩平・足立真佐雄
    • Organizer
      平成26年度日本水産学会春季大会
    • Place of Presentation
      北海道大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 海洋細菌のフォスフォトリエステラーゼ産生能2014

    • Author(s)
      山口晴生・有坂大志・関美樹・外丸裕司・足立真佐雄
    • Organizer
      平成26年度日本水産学会春季大会
    • Place of Presentation
      北海道大学
    • Related Report
      2013 Research-status Report
  • [Presentation] Algal Viral Promoter Useful for Marine Diatom Transformation.2013

    • Author(s)
      Masao Adachi, Takashi Kadono, Arisa Miyagawa-Yamaguchi, Yuji Tomaru, Keizo Nagasaki, Takuma Okami, Nozomu Kira, Kazunari Fukunaga, Haruo Yamaguchi and Kohei Ohnishi
    • Organizer
      EMBO Workshop, The Molecular Life of Diatoms
    • Place of Presentation
      Paris, France
    • Related Report
      2013 Research-status Report
  • [Presentation] 珪藻に感染するウイルス由来プロモーターの活性評価2013

    • Author(s)
      角野貴志・山口亜利沙・外丸裕司・長崎慶三・岡見卓馬・吉良望・福永一成・山口晴生・大西浩平・足立真佐雄
    • Organizer
      珪藻に感染するウイルス由来プロモーターの活性評価
    • Place of Presentation
      沖縄県市長村自治会館
    • Related Report
      2013 Research-status Report
  • [Presentation] 海産珪藻の炭化水素生合成に関わる酵素群のRNA-Seqによる遺伝子発現解析2013

    • Author(s)
      福永一成・吉松孝倫・大西裕美・角野貴志・山口晴生・足立真佐雄
    • Organizer
      第15回マリンバイオテクノロジー学会大会
    • Place of Presentation
      沖縄県市長村自治会館
    • Related Report
      2013 Research-status Report
  • [Presentation] 土佐湾沿岸域における付着性渦鞭毛藻Gambierdiscus属の動態2013

    • Author(s)
      西村朋宏・坂成浩嗣・Wittaya Tawong・上原啓太・井口大輝・池上拓志・中村正利・吉岡拓也・阿部翔太・山口晴生・足立真佐雄
    • Organizer
      2013年日本ベントス学会・日本プランクトン学会合同大会
    • Place of Presentation
      東北大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 海産珪藻Rhizosolenia setigeraの増殖に及ぼす水温、塩分および光強度の影響2013

    • Author(s)
      吉松孝倫・田中愛依・谷知宏・山口晴生・足立真佐
    • Organizer
      平成25年度日本水産学会秋季大会
    • Place of Presentation
      三重大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 海産珪藻Phaeodactylum tricornutum の培養法ならびに増殖生理2013

    • Author(s)
      吉松孝倫・田中愛依・谷知宏・山口晴生・岡内正典・足立真佐雄
    • Organizer
      平成25年度日本水産学会秋季大会
    • Place of Presentation
      三重大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 海産珪藻Chaetoceros属藻が溶存核酸からリン・窒素を多段的に獲得する可能性について2013

    • Author(s)
      小林崇晃・山口晴生・足立真佐雄
    • Organizer
      平成25年度日本水産学会秋季大会
    • Place of Presentation
      三重大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 中心目珪藻Rhizosolenia setigeraの炭化水素及び脂質生産と培地条件の検討2013

    • Author(s)
      石井健一郎・大西晃・足立真佐雄・澤山茂樹
    • Organizer
      平成25年度日本水産学会秋季大会
    • Place of Presentation
      三重大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 15.付着性有毒渦鞭毛藻類2013

    • Author(s)
      足立真佐雄
    • Organizer
      平成25年度水産環境保全委員会研究会「有害有毒プランクトンの分類・生理・生態・生活史・個体群動態」
    • Place of Presentation
      三重大学
    • Related Report
      2013 Research-status Report
  • [Presentation] Algal Viral Promoter Useful for Marine Diatom Transformation.2012

    • Author(s)
      Masao Adachi, Arisa Miyagawa-Yamaguchi, Yuji Tomaru, Keizo Nagasaki, Kazunari Fukunaga, Kohei Ohno, Takuma Okami, Nozomu Kira, Kohei Ohnishi and Haruo Yamaguchi
    • Organizer
      2nd International conference on Algal Biomass, Biofuels and Bioproducts
    • Place of Presentation
      Westin San Diego, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] Effect of temperature change on the dominant species of Gambierdiscus in Japan - From a non-toxic species to a toxic species?2012

    • Author(s)
      Masao Adachi, Takamichi Yoshimatsu, Haruka Iwamoto, Tomohiro Nishimura and Haruo Yamaguchi
    • Organizer
      PICES 2012 Annual Meeting
    • Place of Presentation
      Hiroshima, Japan
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Transformation of the diatom Rhizosolenia setigera using diatom infecting viral promoters.2012

    • Author(s)
      Kazunari Fukunaga, Naoto Hariganeya, Haruo Yamaguchi, Yuji Tomaru, Keizo Nagasaki, Masao Adachi
    • Organizer
      The 9th APMBC2012
    • Place of Presentation
      Kochi, Japan
    • Related Report
      2012 Research-status Report
  • [Presentation] Effect of temperature change on the dominant species of Gambierdiscus in Japan - from a non-toxic species to a toxic species2012

    • Author(s)
      Masao Adachi, Takamichi Yoshimatsu, Haruka Iwamoto, Tomohiro Nishimura and Haruo Yamaguchi
    • Organizer
      15th ICHA 2012 KOREA
    • Place of Presentation
      Gyeongnam, Korea
    • Related Report
      2012 Research-status Report
  • [Presentation] Development of a quantitative PCR assay for the detection and enumeration of the ciguatera-causing dinoflagellate Gambierdiscus spp. in Japanese coastal areas.2012

    • Author(s)
      Tomohiro Nishimura, Naohito Hariganeya, Tawong Wittaya, Hiroshi Sakanari, Yuko Tanimoto, Haruo Yamaguchi and Masao Adachi
    • Organizer
      15th ICHA 2012 KOREA
    • Place of Presentation
      Gyeongnam, Korea
    • Related Report
      2012 Research-status Report
  • [Presentation] Effects of temperature and salinity on growth of toxic dinoflagellate Ostreopsis spp. isolated from Japanese coastal area.2012

    • Author(s)
      Yuko Tanimoto, Haruo Yamaguchi, Takamichi Yoshimatsu and Masao Adachi
    • Organizer
      15th ICHA 2012 KOREA
    • Place of Presentation
      Gyeongnam, Korea
    • Related Report
      2012 Research-status Report
  • [Presentation] The phylogeny of the dinoflagellate Ostreopsis spp. from Thailand.2012

    • Author(s)
      Wittaya Tawong, Tomohiro Nishimura, Hiroshi Sakanari, Haruo Yamaguchi and Masao Adachi
    • Organizer
      15th ICHA 2012 KOREA
    • Place of Presentation
      Gyeongnam, Korea
    • Related Report
      2012 Research-status Report
  • [Presentation] Clarification of growth-irradiance relationships in the toxic Ostreopsis species cultures using the newly devised method.2012

    • Author(s)
      Haruo Yamaguchia, Yuya Tomoria, Yuko Tanimotoa, Masao Adachi
    • Organizer
      15th ICHA 2012 KOREA
    • Place of Presentation
      Gyeongnam, Korea
    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi