The role of autophagy-lysosomal pathway on the developmental competence of bovine oocytes and embryo development
| Project/Area Number |
24580439
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Hokkaido University |
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Principal Investigator |
TAKAHASHI MASASHI 北海道大学, (連合)農学研究科(研究院), 教授 (10343964)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ウシ胚 / 発生 / リソソーム / カテプシン / オートファジー / ウシ / 胚盤胞 / 卵子 |
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| Outline of Final Research Achievements |
This study investigated 1)the relationship between heat shock during in vitro maturation (IVM) of bovine cumulus oocyte complexes (COCs) and cathepsin(CTS) B activity in relation to apoptosis and 2) the effect of supplementation of CTSB inhibitor (E-64) during IVM of heat-shocked COCs on embryonic development were investigated. Heat stress exposure during maturation significantly decreased the cleavage and blastocyst rate as well as increase in lysosome and CTSB activities. Besides, increase of caspase-3 activities in HS-treated oocytes showed significant increase of TUNEL-positive cells in blastocysts.Addition of CTDB inhibitor E-64 to maturation medium significantly increased the developmental rate and total cell number of blastocyst as well as decreasing the activities of lysosome and caspase-3. These results indicate that the regulation of lysosomal CTS by specific inhibitors improve the developmental competence of normal and heat-shocked COCs.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Transcriptional wiring for establishing cell lineage specification at the blastocyst stage in cattle2013
Author(s)
Nagatomo, H., Kagawa, S., Kishi, Y., Takuma, T., Sada, A., Yamanaka, K., Abe, Y., Wada, Y., Takahashi, M., Kono, T., Kawahara, M.
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Journal Title
Biol. Reprod
Volume: Jun 27;88(6)
Issue: 6
Pages: 158-158
DOI
Related Report
Peer Reviewed
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[Journal Article] Production of bioactive bovine fibroblast growth factor 4 in E. coli based on the common nucleotide sequence of its structural gene in three breeds.2013
Author(s)
Sugawara S, Ito T, Sato S, Yokoo M, Mori Y, Kasuga K, Kojima I, Fukuda T, Yamanaka K, Sakatani M, Takahashi M, Kobayashi M.
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Journal Title
Animal Science Journal
Volume: 84
Pages: 275-280
Related Report
Peer Reviewed
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[Journal Article] Production of bioactive bovine fibroblast growth factor 4 in E. coli based on the common nucleotide sequence of its structural gene in three breeds.2013
Author(s)
Sugawara S., Ito T., Sato Sho, Yokoo Mari, Mori Y., Kasuga K., Kojima I., Fukuda T., Yamanaka K., Sakatani M., Takahashi M. and Kobayashi M.
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Journal Title
Animal Science Journal
Volume: 84
Issue: 3
Pages: 275-280
DOI
Related Report
Peer Reviewed
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[Journal Article] Hollow fiber vitrification provides a novel method for cryopreserving in vitro maturation/fertilization-derived porcine embryos2012
Author(s)
Maehara M, Matsunari H, Honda K, Nakano K, Takeuchi Y, Kanai T, Matsuda T, Matsumura Y, Hagiwara Y, Sasayama N, Shirasu A, Takahashi M, Watanabe M, Umeyama K, Hanazono Y, Nagashima H.
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Journal Title
Biology of REproduction
Volume: 87
Issue: 6
Pages: 1-8
DOI
Related Report
Peer Reviewed
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