The role of autophagy-lysosomal pathway on the developmental competence of bovine oocytes and embryo development

• Project/Area Number: 24580439
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Hokkaido University
• Principal Investigator: TAKAHASHI MASASHI 北海道大学, (連合)農学研究科(研究院), 教授 (10343964)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: ウシ胚 / 発生 / リソソーム / カテプシン / オートファジー / ウシ / 胚盤胞 / 卵子

Outline of Final Research Achievements

This study investigated 1)the relationship between heat shock during in vitro maturation (IVM) of bovine cumulus oocyte complexes (COCs) and cathepsin(CTS) B activity in relation to apoptosis and 2) the effect of supplementation of CTSB inhibitor (E-64) during IVM of heat-shocked COCs on embryonic development were investigated. Heat stress exposure during maturation significantly decreased the cleavage and blastocyst rate as well as increase in lysosome and CTSB activities. Besides, increase of caspase-3 activities in HS-treated oocytes showed significant increase of TUNEL-positive cells in blastocysts.Addition of CTDB inhibitor E-64 to maturation medium significantly increased the developmental rate and total cell number of blastocyst as well as decreasing the activities of lysosome and caspase-3. These results indicate that the regulation of lysosomal CTS by specific inhibitors improve the developmental competence of normal and heat-shocked COCs.

Research Products

• [Journal Article] Status of autophagy, lysosome activity and apoptosis during corpus luteum regression in cattle (2015)
  • Author(s): Aboelenain M, Kawahara M, Balboula AZ, Montasser AE, Zaabel SM, Okuda K, Takahashi M
  • Journal Title: Journal of Reproduction and Development Volume: 61 Issue: 3 Pages: 229-236
  • DOI: 10.1262/jrd.2014-135
  • NAID: 130005077734
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Heat stress during in vitro fertilization decreases fertilization success by disrupting anti-polyspermy systems of the oocytes (2015)
  • Author(s): Sakatani M, Yamanaka K, Balboula AZ, Takenouchi N, Takahashi M
  • Journal Title: Molecular Reproduction and Development Volume: 82 Issue: 1 Pages: 36-47
  • DOI: 10.1002/mrd.22441
  • Peer Reviewed / Open Access
• [Journal Article] Restricted development of mouse triploid fetuses with disorganized expression of imprinted genes (2014)
  • Author(s): Yamazaki W, Takahashi M, Kawahara M.
  • Journal Title: Zygote Volume: 16 Issue: 6 Pages: 1-11
  • DOI: 10.1017/s0967199414000550
  • NAID: 120005768767
  • Peer Reviewed / Open Access
• [Journal Article] Expression and regulation of Foxa2 in the rat uterus during early pregnancy (2014)
  • Author(s): Yamagami K, Yamauchi N, Kubota K, Nishimura S, Chowdhury VS, Yamanaka K, Takahashi M, Tabata S, and Hattori MA.
  • Journal Title: Journal of Reproduction and Development Volume: 60 Issue: 6 Pages: 468-475
  • DOI: 10.1262/jrd.2014-086
  • NAID: 130004690752
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed / Open Access
• [Journal Article] Cathepsin B activity has a crucial role in the developmental competence of bovine cumulus-oocyte complexes exposed to heat shock during in vitro maturation. (2013)
  • Author(s): Balboula AZ, Yamanaka K, Sakatani M, Kawahara M,Hegab AO, Zaabel SM, Takahashi M
  • Journal Title: Reproduction Volume: 146 Pages: 407-417
  • Peer Reviewed
• [Journal Article] Dynamics of intracellular phospholipid membrane organization during oocyte maturation and successful vitrification of immature oocytes retrieved by ovum pick-up in cattle. (2013)
  • Author(s): Aono A., Nagatomo H., Takuma T., Nonaka R., Ono Y., Wada Y., Abe Y., Takahashi M., Watanabe T. and Kawahara M.
  • Journal Title: Theriogenology Volume: 79 Pages: 1146-1152
  • NAID: 120005572429
  • Peer Reviewed
• [Journal Article] Transcriptional wiring for establishing cell lineage specification at the blastocyst stage in cattle (2013)
  • Author(s): Nagatomo, H., Kagawa, S., Kishi, Y., Takuma, T., Sada, A., Yamanaka, K., Abe, Y., Wada, Y., Takahashi, M., Kono, T., Kawahara, M.
  • Journal Title: Biol. Reprod Volume: Jun 27;88(6) Issue: 6 Pages: 158-158
  • DOI: 10.1095/biolreprod.113.108993
  • Peer Reviewed
• [Journal Article] Production of bioactive bovine fibroblast growth factor 4 in E. coli based on the common nucleotide sequence of its structural gene in three breeds. (2013)
  • Author(s): Sugawara S, Ito T, Sato S, Yokoo M, Mori Y, Kasuga K, Kojima I, Fukuda T, Yamanaka K, Sakatani M, Takahashi M, Kobayashi M.
  • Journal Title: Animal Science Journal Volume: 84 Pages: 275-280
  • Peer Reviewed
• [Journal Article] Biological Activity of Recombinant Bovine Interferon τ Produced by a Silkworm-Baculovirus Gene Expression System. (2013)
  • Author(s): Takahashi H, Tsunazaki M, Hamano T, Takahashi M, Okuda K, Inumaru S, Okano A, Geshi M, Hirako M.
  • Journal Title: Journal of Veterinary Medical Science Volume: 76 Pages: 447-451
  • NAID: 130003382378
  • Peer Reviewed
• [Journal Article] 家畜の繁殖機能におよぼす暑熱ストレス (2013)
  • Author(s): 高橋昌志
  • Journal Title: 北海道畜産草地学会誌 Volume: 1 Pages: 47-54
  • NAID: 40020240493
• [Journal Article] 家畜の繁殖技術研究の流れと展望 (2013)
  • Author(s): 高橋昌志
  • Journal Title: 日本胚移植学雑誌 Volume: 35 Pages: 1-8
  • NAID: 10031185620
• [Journal Article] Production of bioactive bovine fibroblast growth factor 4 in E. coli based on the common nucleotide sequence of its structural gene in three breeds. (2013)
  • Author(s): Sugawara S., Ito T., Sato Sho, Yokoo Mari, Mori Y., Kasuga K., Kojima I., Fukuda T., Yamanaka K., Sakatani M., Takahashi M. and Kobayashi M.
  • Journal Title: Animal Science Journal Volume: 84 Issue: 3 Pages: 275-280
  • DOI: 10.1111/asj.12036
  • Peer Reviewed
• [Journal Article] Hollow fiber vitrification provides a novel method for cryopreserving in vitro maturation/fertilization-derived porcine embryos (2012)
  • Author(s): Maehara M, Matsunari H, Honda K, Nakano K, Takeuchi Y, Kanai T, Matsuda T, Matsumura Y, Hagiwara Y, Sasayama N, Shirasu A, Takahashi M, Watanabe M, Umeyama K, Hanazono Y, Nagashima H.
  • Journal Title: Biology of REproduction Volume: 87 Issue: 6 Pages: 1-8
  • DOI: 10.1095/biolreprod.112.100339
  • Peer Reviewed
• [Presentation] The role of autophagy and lysosomal functions on reproductive efficiency in cattle (2014)
  • Author(s): MasashiTakahashi
  • Organizer: 4th Hokkaido University-Seoul National Unuversity joint meeting
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2014-11-28
  • Invited
• [Presentation] マウス胎子成長に及ぼす倍数性およびインプリント遺伝子発現の影響 (2014)
  • Author(s): 山崎 渉、河野友宏、高橋昌志、川原 学
  • Organizer: 第１０７回日本繁殖生物学会
  • Place of Presentation: 帯広畜産大学(帯広市)
  • Year and Date: 2014-08-21 – 2014-08-23
• [Presentation] The role of autophagy in regression of bovine corpus luteum in relation to lysosomal fucntion (2014)
  • Author(s): Mansour ABOELENAIN,, Manabu KAWAHARA, Ahmed Zaky BALBOULA, Abd El-monem MONTASSER, Samy Mowaed ZAABEL, Kiyoshi OKUDA, Masashi TAKAHASHI
  • Organizer: 第１０７回日本繁殖生物学会
  • Place of Presentation: 帯広畜産大学(帯広市)
  • Year and Date: 2014-08-21 – 2014-08-23
• [Presentation] 牛子宮内膜組織におけるMX1とMX1B発現に及ぼすI型インターフェロンの応答性およびその受容体の発情周期別比較 (2014)
  • Author(s): 白水貴大、川原 学、木村康二、高橋ひとみ、柳川洋二郎、永野昌志、パイ ルーラン、今川和彦、高橋昌志
  • Organizer: 第１０７回日本繁殖生物学会
  • Place of Presentation: 帯広畜産大学(帯広市)
  • Year and Date: 2014-08-21
• [Presentation] 発情周期に伴うウマ子宮組織内の粘液分泌・血管新生因子およびホルモン受容体遺伝子発現の変動 (2014)
  • Author(s): 菊地康太、香西圭輔、徳山翔太、阪谷美樹、川原 学、南保泰雄、奥田 潔、高橋昌
  • Organizer: 第118回日本畜産学会大会
  • Place of Presentation: 国際会議場エポカル(茨城県つくば市)
• [Presentation] マウス初期胚におけるRNAウイルスレセプターRIG-Iを介した抗ウイルス応答 (2013)
  • Author(s): 佐々木恵亮、高橋昌志、川原学
  • Organizer: 第106回日本繁殖生物学会大会
  • Place of Presentation: 東京農工大学(東京都府中市)
• [Presentation] 赤外線サーモグラフィーによる黒毛和種発情時の体温変動の検出 (2013)
  • Author(s): 高橋昌志、阪谷美樹、川原 学、竹之内直樹
  • Organizer: 第106回日本繁殖生物学会大会
  • Place of Presentation: 東京農工大学(東京都府中市)
• [Presentation] 二母性マウス胚を用いた新規父性メチル化インプリント遺伝子の検証 (2013)
  • Author(s): 山崎 渉、馬狩柚子、河野友宏、高橋昌志、川原 学
  • Organizer: 第106回日本繁殖生物学会大会
  • Place of Presentation: 東京農工大学(東京都府中市)
• [Presentation] 高温成熟条件がウシ卵子および受精卵のTETs、DNMTs発現に与える影響 (2013)
  • Author(s): 阪谷美樹、高橋昌志、竹之内直樹
  • Organizer: 第106回日本繁殖生物学会大会
  • Place of Presentation: 東京農工大学(東京都府中市)
• [Presentation] ウシ初期胚発生の進行に伴うCTGFおよびTEAD4遺伝子の発現動態 (2013)
  • Author(s): 定郁里、長友啓明、高橋昌志、川原学
  • Organizer: 第106回日本繁殖生物学会大会
  • Place of Presentation: 東京農工大学(東京都府中市)
• [Presentation] 暑熱ストレスがウシ体外受精胚のカテプシンB活性および発生能に及ぼす影響 (2013)
  • Author(s): 山中賢一、樫澤 彩、和田康彦、阪谷美樹、竹之内直樹、高橋昌志
  • Organizer: 第106回日本繁殖生物学会大会
  • Place of Presentation: 東京農工大学(東京都府中市)
• [Presentation] 中空糸によるマウス胚盤胞期胚のガラス化凍結保存 (2012)
  • Author(s): 佐野 渚、笹山典久、白数昭雄、太田久由、阪谷美樹、長嶋比呂志、川原 学、高橋昌志
  • Organizer: 第一回北海道畜産草地学会大会
  • Place of Presentation: 北海道大学(札幌)
• [Presentation] ウシ卵子成熟、初期胚発生、品質にかかわるシステインプロアーゼの作用機構 (2012)
  • Author(s): 高橋昌志
  • Organizer: 第１５回 麻布大学 生殖・発生工学セミナー
  • Place of Presentation: 麻布大学(相模原)
  • Invited
• [Book] Frontiers of Agricultural Science (2015)
  • Author(s): Masashi Takahashi
  • Total Pages: 277
  • Publisher: Shoukado
• [Remarks] ようこそ、家畜改良増殖学研究室のホームページへ
  • URL: http://www.agr.hokudai.ac.jp/anim/breed/