Elucidation of genetic factors involving mycotoxin producibility in the fungus parasitic on rice

• Project/Area Number: 24580474
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Boundary agriculture
• Research Institution: Gifu University
• Principal Investigator: SUGA Haruhisa 岐阜大学, 生命科学総合研究支援センター, 准教授 (20283319)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 菌類 / 遺伝子 / マイコトキシン / イネばか苗病菌 / Fusarium

Outline of Final Research Achievements

Fusarium fujikuroi is the fugal pathogen of the rice bakanae disease. Fumonisin is a mycotoxin that causes swine pulmonary edema and equine leukoencephalomalacia. Fumonisin producing population and non-producing population are present in F. fujikuroi. The causative mutations are found to be dispersed in the fumonisin biosynthetic gene cluster in a fumonisin non-producing isolate. One of the mutations is a g.2551G>T(p.G678*) substitution in FUM21 gene coding transcription factor for other fumonisin biosynthetic genes. The coding protein presumed to be disfunctioned by the missing of 11 amino acids at C-terminal derived from the substitution.

Research Products

• [Journal Article] A single nucleotide polymorphism in the translation elongation factor 1α gene correlates with the ability to produce fumonisin in Japanese Fusarium fujikuroi (2014)
  • Author(s): Suga H, Kitajima M, Nagumo R, Tsukiboshi T, Uegaki R, Nakajima T, Kushiro M, Nakagawa H, Shimizu M, Kageyama K, Hyakumachi M
  • Journal Title: Fungal Biologｙ Volume: 118 Issue: 4 Pages: 402-412
  • DOI: 10.1016/j.funbio.2014.02.005
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 主要な植物病原性Fusarium菌の種分類の現状と簡易菌種同定法の利用例 (2014)
  • Author(s): 須賀晴久
  • Journal Title: 植物防疫 Volume: 68 Pages: 269-273
  • NAID: 40020085053
  • Acknowledgement Compliant
• [Journal Article] Root and crown rot of strawberry caused by Pythium helicoides and its distribution in strawberry production areas of Japan (2014)
  • Author(s): IshiguroY, Otsubo K, Watanab H, Suzuki M, Nakayama K, Fukuda T, Fujinaga M, Suga H, Kageyama K
  • Journal Title: Journal of General Plant Pathology Volume: 80 Issue: 5 Pages: 423-429
  • DOI: 10.1007/s10327-014-0520-8
  • Peer Reviewed
• [Journal Article] Monitoring by real-time PCR of three water-borne zoosporic Pythium species in potted flower and tomato greenhouses under hydroponic culture systems (2014)
  • Author(s): Li M, Ishiguro Y, Otsubo K, Suzuki H, Tsuji T, Miyake N, Nagai H, Suga H, Kageyama K
  • Journal Title: European Journal of Plant Pathology Volume: 140 Issue: 2 Pages: 229-242
  • DOI: 10.1007/s10658-014-0456-z
  • Peer Reviewed
• [Journal Article] Phytophthora asiatica sp. nov., a new species causing leaf and stem blight of kudzu in Japan (2014)
  • Author(s): Rahman MZ, Mukobata H, Suga H, Kageyama K
  • Journal Title: Mycological Progress Volume: 13 Issue: 3 Pages: 759-769
  • DOI: 10.1007/s11557-014-0959-1
  • Peer Reviewed
• [Journal Article] Re-evaluation of Japanese Phytophthora isolates based on molecular phylogenetic analyses (2014)
  • Author(s): Rahman MZ, Uematsu S, Coffey MD,Uzuhashi S, Suga H, Kageyama K
  • Journal Title: Mycoscience Volume: 55 Issue: 4 Pages: 314-327
  • DOI: 10.1016/j.myc.2013.11.005
  • Peer Reviewed
• [Journal Article] Two new species, Phytophthora nagaii sp. nov. and P. fragariaefoilia sp. nov., causing seriuos diseases on rose and strawberry in Japan (2014)
  • Author(s): Rahman MZ, Uematsu S, Takeuchi T, Shirai K, Ishiguro Y, Suga H, Kageyama K
  • Journal Title: Journal of General Plant Pathology Volume: 80 Issue: 4 Pages: 348-365
  • DOI: 10.1007/s10327-014-0519-1
  • Peer Reviewed
• [Journal Article] Phylogenetic relationships among Phytopythium species, and re-evaluation of Phytopythium fagopyri comb. nov., recovered from damped-off buckwheat seedlings in Japan (2014)
  • Author(s): Baten MA, Asano T, Motohashi K, Ishiguro Y, Rahman MZ, Inaba S, Suga H, Kageyama K
  • Journal Title: Mycological Progress Volume: 13 Issue: 4 Pages: 1145-1156
  • DOI: 10.1007/s11557-014-1003-1
  • Peer Reviewed
• [Journal Article] Development of PCR primers to identify Fusarium oxysporum f. sp. fragariae. (2013)
  • Author(s): Suga H, Hirayama Y, Morishima M, Suzuki T, Kageyama K, Hyakumachi M
  • Journal Title: Plant Disease Volume: 97 Issue: 5 Pages: 619-625
  • DOI: 10.1094/pdis-07-12-0663-re
  • Peer Reviewed
• [Journal Article] Simultaneous Detection and Quantification of <i>Phytophthora nicotianae</i> and <i>P. cactorum</i>, and Distribution Analyses in Strawberry Greenhouses by Duplex Real-time PCR (2013)
  • Author(s): Li M, Inada M, Watanabe H, Suga H, Kageyama K
  • Journal Title: Microbes and Environments Volume: 28 Issue: 2 Pages: 195-203
  • DOI: 10.1264/jsme2.ME12177
  • NAID: 10031158622
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed
• [Journal Article] Simultaneous detection by multiplex PCR of the high-temperature-growing Pythium species: P. aphanidermatum, P. helicoides and P. myriotylum. (2013)
  • Author(s): Ishiguro Y, Asano T, Otsubo K, Suga H, Kageyama K
  • Journal Title: Journal of General Plant Pathology Volume: 79 Issue: 5 Pages: 350-358
  • DOI: 10.1007/s10327-013-0466-2
  • NAID: 10031195199
  • Peer Reviewed
• [Journal Article] Fusarium azukicola sp. nov., an exotic azuki bean root-rot pathogen in Hokkaido, Japan (2012)
  • Author(s): T. Aoki
  • Journal Title: Mycologia Volume: 104 Issue: 5 Pages: 1068-1084
  • DOI: 10.3852/11-303
  • Peer Reviewed
• [Journal Article] Mycoheterotrophic germination of Pyrola asarifolia dust seeds reveals convergences with germination in orchids (2012)
  • Author(s): Hashimoto Y, Fukukawa S, Kunishi A, Suga H, Richard F, Sauve M, Selosse M-A
  • Journal Title: New Phytologist Volume: 195 Issue: 3 Pages: 620-630
  • DOI: 10.1111/j.1469-8137.2012.04174.x
  • Peer Reviewed
• [Presentation] エジプトのイネから分離されたFusarium fujikuroi種複合体の菌種構成とジベレリン及びフモニシン産生 (2015)
  • Author(s): Elkot Gabr A., Elsharkawy Mohsen M., 船坂美佳, 清水将文, 景山幸二, 百町満朗, 須賀晴久
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 明治大学 (東京)
  • Year and Date: 2015-03-29
• [Presentation] Fusarium fujikuroiに見られるジベレリン産生力の違いの原因マッピング (2015)
  • Author(s): 長坂拓弥, 新井満大, 北嶋美葉, 景山幸二, 清水将文, 百町満朗, 須賀晴久
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 明治大学 (東京)
  • Year and Date: 2015-03-29
• [Presentation] Fusarium fujikuroiのフモニシン産生能喪失に関わるFUM21遺伝子中の変異 (2015)
  • Author(s): 須賀晴久, 船坂美佳, 景山幸二, 清水将文, 百町満朗
  • Organizer: 第76回日本マイコトキシン学会学術講演会
  • Place of Presentation: 千葉大学(千葉県)
  • Year and Date: 2015-02-06
• [Presentation] Fusarium fujikuroi におけるフモニシン産生株と非産生株のジベレリン産生性の違い (2014)
  • Author(s): 須賀晴久, 新井満大, 瀧瀬康子, 船坂美佳, 竪石秀明, 清水将文, 景山幸二, 百町満朗
  • Organizer: 日本植物病理学会西部会
  • Place of Presentation: 富山大学(富山県)
  • Year and Date: 2014-09-27
• [Presentation] イネ種子から分離された小型分生胞子連鎖状形成Fusarium属菌の種とフモニシン産生能 (2014)
  • Author(s): 須賀晴久, 船坂美佳, 清水将文, 景山幸二, 百町満朗
  • Organizer: 日本菌学会58回大会
  • Place of Presentation: サイエンスヒルズこまつ(石川県)
  • Year and Date: 2014-06-15
• [Presentation] ムギ類赤かび病菌におけるFGSG02810遺伝子破壊による病原性と子のう殻形成能の喪失 (2014)
  • Author(s): 須賀晴久, 船坂美佳, 清水将文, 景山幸二, 百町満朗
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2014-06-02
• [Presentation] 遺伝子導入によるFusarium fujikuroi G系統株のフモニシン産生能の回復
  • Author(s): 須賀晴久, スコット暁子, 小林弘典, 景山幸二, 清水将文, 百町満朗
  • Organizer: 第74回日本マイコトキシン学会学術講演会
  • Place of Presentation: 麻布大学(神奈川県)
• [Presentation] 国内分離株に見られるFusarium fujikuroiの2系統と国外分離株との関係
  • Author(s): 須賀晴久, 新井満大, 船坂美佳, 清水将文, 景山幸二, 百町満朗
  • Organizer: 日本植物病理学会西部会
  • Place of Presentation: 岡山大学(岡山県)
• [Presentation] Fusarium fujikuroiのG系統株に見出だされたフモニシン産生遺伝子クラスターの部分欠失とFUM遺伝子の発現異常
  • Author(s): 須賀晴久, スコット暁子, 景山幸二, 北嶋美葉, 清水将文, 百町満朗
  • Organizer: 第73回日本マイコトキシン学会学術講演会
  • Place of Presentation: 大阪府立大学(大阪府)
• [Presentation] Fusarium fujikuroi に見出されたイネばか苗病を起こさない系統
  • Author(s): 須賀晴久, 船坂美佳, 深澤恵海, 新井満大, 清水将文, 景山幸二, 百町満朗
  • Organizer: 日本植物病理学会
  • Place of Presentation: 岐阜大学(岐阜県)
• [Presentation] Fusarium fujikuroiに見られるフモニシン非産生株のフモニシン産生遺伝子クラスター領域の構造
  • Author(s): 山口竜央, スコット暁子, 景山幸二, 百町満朗, 須賀晴久
  • Organizer: 第72回日本マイコトキシン学会学術講演会
  • Place of Presentation: 東京家政大学(東京都)
• [Presentation] Fusarium fujikuroi におけるフモニシン非産生の原因マッピング
  • Author(s): 須賀晴久, 北嶋美葉, スコット暁子, 船坂美佳, 景山幸二, 百町満朗
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: とりぎん文化会館(鳥取県)
• [Presentation] チオファネートメチル耐性ムギ類赤かび病菌のトリコテセン系マイコトキシン産生に対するテブコナゾールの影響について
  • Author(s): 須賀晴久, 船坂美佳, 景山幸二, 百町満朗, 平八重一之
  • Organizer: 第71回日本マイコトキシン学会学術講演会
  • Place of Presentation: 沖縄県市町村自治会館(沖縄県)