Development of auxiliaries for ligation of peptides at a site other than cystein
| Project/Area Number |
24590010
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemical pharmacy
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| Research Institution | The University of Tokushima |
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Principal Investigator |
SHIGENAGA Akira 徳島大学, 大学院ヘルスバイオサイエンス研究部, 講師 (10423394)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | Native Chemical Ligation / フラグメント縮合 / タンパク質化学合成 / 光解離性補助基 / NCL / タンパク質化学 / ペプチド化学 |
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| Outline of Final Research Achievements |
In this study, development of auxiliaries for ligation of peptides at a site other than cystein was challenged. The auxiliary that can be removed by UV-irradiation was syntheseized and was incorporated onto a side chain of asparagine (Asn). Ligation of peptides at Xaa-Asn site was successfully achieved by the use of the Asn derivative via auxiliary assisted ligation followed by photo-removal of the auxiliary. Application of the auxiliary to amino acids other than Asn is in progress.
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Report
(4 results)
Research Products
(46 results)
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[Journal Article] Development of a fluoride-responsive amide bond cleavage device that is potentially applicable to a traceable linker2014
Author(s)
J. Yamamoto, N. Maeda, C. Komiya, T. Tanaka, M. Denda, K. Ebisuno, W. Nomura, H. Tamamura, Y. Sato, A. Yamauchi, A. Shigenaga, and A. Otaka
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Journal Title
Tetrahedron
Volume: 70
Issue: 34
Pages: 5122-5127
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Development of a traceable linker containing a thiol-responsive amino acid for the enrichment and selective labelling of target proteins2014
Author(s)
J. Yamamoto, M. Denda, N. Maeda, M. Kita, C. Komiya, T. Tanaka, W. Nomura, H. Tamamura, Y. Sato, A. Yamauchi, A. Shigenaga, and A. Otaka
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Journal Title
Org. Biomol. Chem.
Volume: 12
Issue: 23
Pages: 3821-3826
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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