Regulatory mechanism of protein assembly in cell membranes

• Project/Area Number: 24590043
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Tohoku University
• Principal Investigator: MIURA Takashi 東北大学, 薬学研究科(研究院), 准教授 (30222318)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 脂質膜 / タンパク質 / ラマン分光法 / 蛍光分光法 / 円偏光二色性 / 構造生物学

Outline of Final Research Achievements

Effects of proteins on the structure and fluidity of lipid membranes were investigated to reveal the regulatory mechanism of protein assembly on cell membranes. Several studies have reported that Abeta peptide decreases fluidity of membranes based on an Abeta-induced increase in the fluorescence anisotropy of diphenylhexatriene (DPH). However, this study showed that the extraction of DPH by peptides or proteins can disturb the estimation of membrane fluidity by using the fluorescence anisotropy of DPH. Therefore we developed novel methods to investigate the structures and physical properties of lipid membranes by using Raman and CD spectroscopies.

Research Products

• [Journal Article] Effect of amyloid β-peptide on the fluidity of phosphatidylcholine membranes: Uses and limitations of diphenylhexatriene fluorescence anisotropy (2015)
  • Author(s): Masako Suzuki and Takashi Miura
  • Journal Title: Biochim. Biophys. Acta Volume: 1848 Issue: 3 Pages: 753-759
  • DOI: 10.1016/j.bbamem.2014.12.003
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] His-Trp Cation-π Interaction and Its Structural Role in an α-Helical Dimer of HIV-1 Vpr Protein (2013)
  • Author(s): Takayuki Kamiyama, Takashi Miura, and Hideo Takeuchi
  • Journal Title: Biophysical Chemistry Volume: 173-174 Pages: 8-14
  • DOI: 10.1016/j.bpc.2013.01.004
  • Peer Reviewed
• [Journal Article] Raman spectroscopic detection of the T-HgII-T base pair and the ionic characteristics of mercury (2012)
  • Author(s): Tomomi Uchiyama, Takashi Miura, Hideo Takeuchi, Takenori Dairaku, Tomoyuki Komuro, Takuya Kawamura, Yoshinori Kondo, Ladislav Benda, Vladimir Sychrovsky, Petr Bour, Itaru Okamoto, Akira Ono and Yoshiyuki Tanaka
  • Journal Title: Nucleic Acids Res Volume: 40 Issue: 12 Pages: 5766-5774
  • DOI: 10.1093/nar/gks208
  • Peer Reviewed
• [Presentation] diphenylhexatrieneを用いる脂質膜流動性測定に与えるAβペプチドの影響 (2014)
  • Author(s): Masako Suzuki, Takashi Miura, Takakazu Nakabayashi
  • Organizer: 第 52 回日本生物物理学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 変性apo-SOD1 のCu 結合部位におけるHis 残基の帰属 (2014)
  • Author(s): Nobuhiro Fujimaki, Takashi Miura, Takakazu Nakabayashi
  • Organizer: 第 52 回日本生物物理学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 家族性ALS に関連したA4V、G93A 変異SOD1 の酸化促進性獲得 (2014)
  • Author(s): Ken Nishiya, Nobuhiro Fujimaki, Furi Kitamura, Takashi Miura, Takakazu Nakabayashi, Hideo Takeuchi
  • Organizer: 第 52 回日本生物物理学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 脂質膜の物理的性質とアミロイド線維形成の関係 (2013)
  • Author(s): 三浦隆史, 鈴木麻紗子
  • Organizer: 第51回 日本生物物理学会年会
  • Place of Presentation: 京都
  • Invited
• [Presentation] 蛍光異方性を利用した脂質膜流動性測定に与えるAβペプチドの影響 (2013)
  • Author(s): 鈴木麻紗子、三浦隆史
  • Organizer: 第52回 日本薬学会東北支部大会
  • Place of Presentation: 仙台
• [Presentation] Role of Tryptophan Residues in Cu(I) Binding of Fission Yeast Copper Transport Proteins (2013)
  • Author(s): Mariko Okada and Takashi Miura
  • Organizer: 7th International Conference on Advanced Vibrational Spectroscopy
  • Place of Presentation: 神戸
• [Presentation] 銅輸送タンパク質Ctr4 の一価銅安定化作用における芳香族性側鎖の役割 (2013)
  • Author(s): 岡田毬子、三浦隆史
  • Organizer: 第52回 日本薬学会東北支部大会
  • Place of Presentation: 仙台
• [Presentation] 家族性ALS に関連した変異SOD1 のミスフォールディングと酸化促進性獲得 (2013)
  • Author(s): 西屋健、北村富里、三浦隆史、竹内英夫
  • Organizer: 第52回 日本薬学会東北支部大会
  • Place of Presentation: 仙台
• [Presentation] 神経変性疾患原因ペプチドの脂質膜結合に対する膜相状態の影響 (2012)
  • Author(s): 三浦隆史、依田真由美、東海林秀樹、志賀聡司、竹内英夫
  • Organizer: 第34回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 京都
• [Presentation] プリオンタンパク質神経毒性セグメントと酸性脂質膜の結合に対する膜相状態の影響
  • Author(s): 志賀聡司、三浦隆史、竹内英夫
  • Organizer: 第51回日本薬学会東北支部大会
  • Place of Presentation: 青森