Voltage-dependent structural changes and functinal mechanism of voltage-dependent ion channels
| Project/Area Number |
24590048
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | The University of Tokyo |
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Principal Investigator |
OSAWA Masanori 東京大学, 薬学研究科(研究院), 講師 (60361606)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMADA Ichio 東京大学, 大学院薬学系研究科, 教授 (70196476)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 電位依存性イオンチャネル / SS-locking / 構造変化 / 電位依存性K+チャネル / 膜電位依存性 / NMR / rHDL |
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| Outline of Final Research Achievements |
Voltage-dependent K+ channels (Kv) regulates active potential by selectively permeating K+ through cell membrane, playing critical roles in neurotransmission and muscle contraction. To date, voltage-dependent confomational changes of Kv remais elusive, due to lack of the method to analyze the strucutre under membrane potential.
The aim of this study is to reveal structrural mechanism of Kv that senses membrane potential. We established a novel method to analyze structure under membrane potential. Based on the results obtained here, we propose that Kv undergoes structural equilibrium that shifts depending upon the membrane potential.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Structural basis for the binding of the membrane-proximal C-terminal region of chemokine receptor CCR2 with the cytosolic regulator FROUNT2014
Author(s)
Esaki K, Yoshinaga S, Tsuji T, Toda E, Terashima Y, Saitoh T, Kohda D, Kohno T, Osawa M, Ueda T, Shimada I, Matsushima K, Terasawa H
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Journal Title
FEBS J
Volume: 281
Issue: 24
Pages: 552-566
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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