The development study of highly sensitive chemiluminescent probe for tumor hypoxia in vivo imaging
| Project/Area Number |
24590054
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Gifu Pharmaceutical University |
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Principal Investigator |
OKUDA Kensuke 岐阜薬科大学, 薬学部, 准教授 (00311796)
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| Co-Investigator(Kenkyū-buntansha) |
NAGASAWA Hideko 岐阜薬科大学, 薬学部, 教授 (90207994)
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| Co-Investigator(Renkei-kenkyūsha) |
KONDO Shinae 東京工業大学, 生命理工学研究科, 教授 (40314182)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 化学発光 / 分析科学 / 低酸素 / イメージング / 高感度 / 低侵襲性 / 非侵襲性 / がん / インビボ / 癌 |
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| Outline of Final Research Achievements |
Chemiluminescence imaging has the advantage of lower detection limits and improved signal-to-noise ratio compared to existing fluorescent imaging. To visualize the hypoxic status of various tumor cells which are considered to be closely related to malignancy, I developed a probe that emit chemiluminescence, which is suitable for non-invasive bioimaging, under hypoxia. To utilize hypoxia selective reductive metabolic reaction, I designed and synthesized chemiluminescent probe molecules rationally. Then, I evaluated them using nitroreductase to select the molecule to emit luminescence from the reaction. Next, I analyzed reaction products to show the molecule worked as the enzyme substrate to emit luminescence. As a result, I obtained significant expertise to apply the molecule for in vivo chemiluminescent imaging.
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Report
(4 results)
Research Products
(15 results)
