Nano vectors with biosurfactant MEL-A

• Project/Area Number: 24590060
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Aichi Gakuin University
• Principal Investigator: NAKANISHI MAMORU 愛知学院大学, 薬学部, 教授 (90090472)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Hiromitsu 愛知学院大学, 薬学部, 教授 (30275094) ; INHO Yoshikazu 愛知学院大学, 薬学部, 講師 (90434547)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: nano vectors / liposome / biosurfactant / gene transfection / medical treatment / membrane fusion / ナノ遺伝子ベクター / バイオ・ナノ遺伝子 / 正電荷リポソイーム / バイオサーファクタント / 遺伝子導入 / レーザ顕微鏡 / 金ナノ粒子 / RNA干渉 / 正電荷リポソーム / ＲＮＡ干渉

Outline of Final Research Achievements

The liposomes made by biosurfactant(MEL-A) dramatically increased the efficiency in transfection of plasmid DNAs and foreign DNAs. The efficiency by nano vector is derived from the unique pathway similar to that by certain RNA virus vectors. From the safety and low toxicity the nano vector would appear to be a new operating technique with great potential in gene transfection and drug delivery in medical treatment.

Research Products

• [Journal Article] Phosphorylation of syntaxin-3 at Thr 14 negatively regulates exocytosis in RBL-2H3 cells (2016)
  • Author(s): Tadokoro, S., Sibata, T., Inoh, Y., Amano, T., Nakanishi, M., Hirashima, N.
  • Journal Title: Cell Biol. Int., Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Impaired expression of the mitochondrial calcium (2015)
  • Author(s): Furuno, T., Shinkai, N., Inoh, Y.., Nakanishi, M.
  • Journal Title: Mol. Cell.Biochem. Volume: 410 Issue: 4 Pages: 215-221
  • DOI: 10.1002/cbf.3185
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Effects of PIP2 on membrane fusion between mast cell SNARE liposomes (2015)
  • Author(s): Tadokoro, S.., Inoh, Y., Nakanishi, M.,Hirashima, N.
  • Journal Title: Biochim. Biophys. Acta. Volume: 1848 Pages: 2290-2294
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Role of cathepsin E from decidual macrophages (2014)
  • Author(s): S. Goto, Y. Ozaki, N. Suzumori, M. Nakanishi
  • Journal Title: Mol. Hum. Reprod. Volume: 20 Pages: 454-462
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Role of cathepsin E from decidual macrophages (2014)
  • Author(s): Goto, S., Ozaki, Y., Furuno, T., Nakanishi, M.
  • Journal Title: Mol. Hum. Reprod., Volume: 20
  • Peer Reviewed
• [Journal Article] Increased expression of cell adhesion molecule 1. (2013)
  • Author(s): Hagiyama, M., Inoue, T., Furuno, T., Iino, T.. Nakanishi.，M.
  • Journal Title: Br. J. Dermatol., Volume: 168 Pages: 771-778
  • Peer Reviewed
• [Journal Article] Synergistic effect of a biosurfactant and protamine on gene transfection. (2013)
  • Author(s): Inoh, Y., Furuno, T., Hirashima, N., Kitamoto, D., Nakanishi, M.
  • Journal Title: Eur. J. Pharm. Sci. Volume: 49 Pages: 1-9
  • Peer Reviewed
• [Journal Article] New transffection agents based on liposomes containing biosurfactant MEL-A. (2013)
  • Author(s): Nakanishi, M., Inoh, Y., Furuno, T.
  • Journal Title: Pharmaceutics Volume: 5 Pages: 411-420
  • Peer Reviewed
• [Journal Article] Substance P is an important role in cell adhesion 1-mediated nerve-pancreatic alpha cell interaction. (2013)
  • Author(s): Nakamura, M., Inoh, Y., Nakanishi, M., Furuno, T.,
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 438 Pages: 563-567
  • Peer Reviewed
• [Journal Article] Inhibitory effects of a cationic liposome on allergic reaction. (2013)
  • Author(s): Inoh, Y., Tadokoro, S., Tanabe, H., Inoue,M., Nakanishi., M., Furuno. T.
  • Journal Title: Biochem. Pharmacol. Volume: 86 Pages: 1731-1738
  • Peer Reviewed
• [Journal Article] 肥満細胞と感覚神経の接着におけるCADM1とネクチン3の関与 (2013)
  • Author(s): 中西 守、古野忠秀
  • Journal Title: 生体の科学 Volume: 64 Pages: 216-219
  • Peer Reviewed
• [Journal Article] Increased expression of cell adhesion molecule 1 by mast cells. (2013)
  • Author(s): Hagiyama, M., Inoue, T., Furuno, T., Nakanishi, M.
  • Journal Title: Br. J. Dermatol. Volume: 168 Pages: 771-778
  • Peer Reviewed
• [Journal Article] Synergistic effect of a biosurfactant and protamine on gene transfecion efficiency.. (2013)
  • Author(s): Inoh, Y., Furuno, T., Nakanishi, M.
  • Journal Title: Eur. J. Pharm. Sci., Volume: 49 Pages: 1-9
  • Peer Reviewed
• [Journal Article] Kefiran suppresses antigen-inducednmastncellnactivation. (2012)
  • Author(s): furuno, T., Nakanishi, M.
  • Journal Title: Biol. Pharm. Bull. Volume: 35 Pages: 178-183
  • Peer Reviewed
• [Journal Article] Cell adhesion molecule 1 (CADM1) on mast cells promotes interacion with DRG. (2012)
  • Author(s): Furuno, T., Hagiyama, M., Nakanishi, M.
  • Journal Title: Neuroimmunol. Volume: 250 Pages: 50-58
  • Peer Reviewed
• [Journal Article] Mitochondria take up Ca ion in twon steps. (2012)
  • Author(s): Takekawa, M., Furuno, T., Nakanishi, M.
  • Journal Title: Biol. Pharm. Bull. Volume: 35 Pages: 1354-1360
  • Peer Reviewed
• [Presentation] MEL-A含有正電荷リポソイームによる樹状細胞へのタンパク質導入 (2016)
  • Author(s): 伊納義和、古野忠秀、中西 守
  • Organizer: 日本薬学会第１３６年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-03-28
• [Presentation] マスト細胞の脱顆粒に及ぼすミトコンドリアカルシュウムユニポーターの影響 (2016)
  • Author(s): 古野忠秀、伊納義和、中西 守
  • Organizer: 日本薬学会第１３６年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-03-28
• [Presentation] 細胞接着を介した神経によるアルファー細胞の機能解析 (2015)
  • Author(s): T. Furuno, Y. Inoh, N. Hirashima, M. Nakanishi
  • Organizer: 日本薬学会第１３５年会
  • Place of Presentation: 神戸サンボーホール
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] マスト細胞の脱顆粒におけるリン酸化の役割 (2015)
  • Author(s): T. Tadokoro, Y. Inoh, M. Nakanishi, N. Tate
  • Organizer: 日本薬学会第１３５年会
  • Place of Presentation: 神戸サンボーホール
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] Analysis of granule movement in pancreatic islet (2014)
  • Author(s): T. Furuno, Y. Inoh, N. Hirashima, M. Nakanishi
  • Organizer: 日本生物物理学会第５２年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 正電荷リポソームによる即時型アレルギー反応への影響 (2014)
  • Author(s): 伊納義和、田所 哲、古野忠秀、中西 守
  • Organizer: 日本薬学会年会
  • Place of Presentation: 熊本大学
• [Presentation] 共存培養系を用いた神経と膵島アルファー細胞の相互作用 (2014)
  • Author(s): 古野忠秀、中村真央、伊納義和、中西 守
  • Organizer: 日本薬学会年会
  • Place of Presentation: 熊本大学
• [Presentation] MEL-A含有正電荷リポソーム (2013)
  • Author(s): 伊納義和、古野忠秀、平嶋尚英、中西 守
  • Organizer: 日本薬学会年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 接着分子CADM1の接着力と相互作用 (2013)
  • Author(s): 古野忠秀、伊納義和、中西 守
  • Organizer: 日本薬学会年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 共存培養系を用いた細胞間相互作用の可視化解析 (2013)
  • Author(s): 横川 慧、伊納義和、古野忠秀、中西守
  • Organizer: 日本薬学会年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] ＭＥＬ－Ａ含有正電荷リポソームによるプロタミン／ＤＮＡの遺伝子導入 (2013)
  • Author(s): 伊納義和、古野忠秀、中西 守
  • Organizer: 日本薬学会第１３３回年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 接着分子ＣＡＤＭ１が神経細胞とマスト細胞の接着力及び相互作用に及ぼす影響 (2013)
  • Author(s): 古野忠秀、伊納義和、中西 守
  • Organizer: 日本薬学会第１３３回年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 共存培養系を用いた細胞間相互作用の可視化解析 (2013)
  • Author(s): 横川 慧、伊納義和、古野忠秀
  • Organizer: 日本薬学会第１３３回年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Cell adhesion molecule 1 (CADM1) on mast cells promotes interaction with dorsal root ganglion neurites by heterophilic binding to nectin-3 (2012)
  • Author(s): Furuno, T., Inoh, Y., Nakanishi, M.
  • Organizer: 日本生物物理学会第５０年会
  • Place of Presentation: 名古屋大学東山キャンパス
• [Presentation] Promotion of DNA delivery into nucleus dramatically enhances the transfection efficiency mediated by biosurfactant -containing (2012)
  • Author(s): Inoh, Y., Furuno, T., Nakanishi, M.
  • Organizer: 日本生物物理学会第５０年会
  • Place of Presentation: 名古屋大学東山キャンパス