Mass spectrometry imaging of single hair specimens for the reliable chronological estimation of drug abuse, and for the investigation of the mechanism of drug incorporation into hair

• Project/Area Number: 24590065
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Osaka Medical College
• Principal Investigator: TSUCHIHASHI Hitoshi 大阪医科大学, 医学部, 准教授 (40596029)
• Co-Investigator(Renkei-kenkyūsha): NAKANISHI Toyofumi 大阪医科大学, 医学部, 准教授 (10247843) ; SATO Takako 大阪医科大学, 医学部, 講師 (10530420)
• Research Collaborator: MIKI Akihiro 大阪医科大学, 法医学, 特別協力研究員 ; KAMATA Tooru 大阪医科大学, 法医学, 特別協力研究員 ; SHIMA Noriaki 大阪医科大学, 法医学, 特別協力研究員 ; ZAITSU Kei 大阪医科大学, 法医学, 特別協力研究員 ; KATAGI Munehiro 大阪府警察本部, 科学捜査研究所, 主席研究員
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 毛髪 / 乱用薬物 / 質量分析 / イメージング / 毛髪分析 / TOF/TOF

Outline of Final Research Achievements

A novel mass spectrometry imaging procedure was established for the visualization of drugs incorporated into hair. Mass spectrometry imaging was performed on the longitudinal sections of single scalp hair shafts from volunteers who took an OTC medicine containing methoxyphenamine, a model drug of methamphetamine. Drug positive bands were observed on the hair specimens in accordance with their schedule of administration. To investigate the mechanism of drug incorporation into hair, single hair specimens were collected by plucking out with the roots intact, and analyzed by the established method.

Research Products

• [Journal Article] Single-hair analysis of zolpidem on the supposition of its single (2015)
  • Author(s): Shima, N.; Sasaki, K.; Kamata, T.; Matsuta, S.; Katagi, M.; Miki,
  • Journal Title: Forensic Toxicol. Volume: 33 Issue: 1 Pages: 122-130
  • DOI: 10.1007/s11419-014-0260-7
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Time-Course Mass Spectrometry Imaging for Depicting Drug Incorporation into Hair (2015)
  • Author(s): Tooru Kamata, Noriaki Shima, Keiko Sasaki, Shuntaro Matsuta, Shiori Takei, Munehiro Katagi, Akihiro Miki, Kei Zaitsu, Toyofumi Nakanishi, Takako Sato, Koichi Suzuki, and Hitoshi Tsuchihashi
  • Journal Title: Analytical Chemistry (ACS Publications) Volume: - Issue: 11 Pages: 5476-5481
  • DOI: 10.1021/acs.analchem.5b00971
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 毛髪の質量分析イメージングで探る薬物使用歴 (2013)
  • Author(s): 三木昭宏 土橋均 等
  • Journal Title: 臨床化学 Volume: 42 Pages: 311316-311316
• [Presentation] Time-Course Mass Spectrometry Imaging for Depicting Drug Incorporation into Hair (2014)
  • Author(s): Noriaki Shima, Tooru Kamata, Akihiro Miki, Munehiro Katagi, Kei Zaitsu, Takako Sato,
  • Organizer: TIAFT (The International Association of Forensic Toxicologists TIAFT2014 Meeting)（TIAFT 2014）国際法中毒学会
  • Place of Presentation: Buenos Airis, Argentina ブエノスアイレス（アルゼンチン）
  • Year and Date: 2014-11-11
• [Presentation] 質量分析イメージングによる毛髪中薬物の分析 (2014)
  • Author(s): 三木昭宏, 鎌田徹, 志摩典明, 片木宗弘, 中西豊文, 土橋均
  • Organizer: 日本法中毒学会第33年会 シンポジウム
  • Place of Presentation: 名古屋
  • Year and Date: 2014-07-04
  • Invited
• [Presentation] イメージング質量分析による毛髪中メトキシフェナミンの画像化 (2013)
  • Author(s): 鎌田徹 土橋均 等
  • Organizer: 日本法中毒学会第32年会
  • Place of Presentation: 千葉県柏市 さわやかちば県民プラザ
• [Presentation] Hair Analysis for Drugs by Mass Spectrometry Imaging : A New Perspective to Investigate Drug Use History. (2013)
  • Author(s): 三木昭宏 土橋均 等
  • Organizer: TIAFT 2013
  • Place of Presentation: ポルトガル（フンシャル）
• [Presentation] Imaging Hair Analyses for Drugs:A New Perspective to Investigate Drug Use History.
  • Author(s): 鎌田 徹 土橋 均 等
  • Organizer: 第19回 国際質量分析学会
  • Place of Presentation: 国立京都国際会館（京都）
• [Presentation] イメージング質量分析による毛髪試料中薬物の検出
  • Author(s): 鎌田 徹 土橋 均 等
  • Organizer: 日本法科学技術学会 第１８回学術集会
  • Place of Presentation: ホテルフロラシオン青山（東京）