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Analysis and search of novel signal transduction-related molecules targeting a broad spectrum of antiviral drugs

Research Project

Project/Area Number 24590167
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental pharmacy
Research InstitutionYasuda Women's University

Principal Investigator

MORIMOTO Kinjiro  安田女子大学, 薬学部, 教授 (80183664)

Co-Investigator(Kenkyū-buntansha) SATO Yuichiro  安田女子大学, 薬学部・薬学科, 講師 (60416427)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsウイルス感染 / レクチン / 抗ウイルス活性 / 自然免疫 / ウイルス
Outline of Final Research Achievements

In early stages (4hpi and 8hpi) of infection with influenza virus, changes in expression of the cellular genes were analyzed using human lung cell lines (A549 cells and NCI-H292 cells). Mannose-binding lectin PFL (derived from Pseudomonas fluorescens Pf01 strain), which inhibited virus-invasion into the host cells due to its binding with HA protein on the envelope of influenza virus particle, was examined for effect of the cellular gene expression.
PML gene (lectin derived from Pseudomonas mandelii) and PTL gene (lectin derived from Pseudomonas taiwanensis) were cloned, expressed in E coli, and the gene products were purified. These lectins were examined for the inhibition of influenza virus infection. PFL was also found to have an activity for the invasion inhibition of feline immunodeficient virus.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (14 results)

All 2015 2014 2013 2012

All Journal Article (6 results) (of which Peer Reviewed: 5 results) Presentation (8 results)

  • [Journal Article] Sequence analysis of the CXCR4 gene derived from cells surviving feline lentivirus infection.2014

    • Author(s)
      Tadafumi S Tochikura, Kinjiro Morimoto
    • Journal Title

      Archives of Virology

      Volume: 45 Issue: 6 Pages: 1511-1513

    • DOI

      10.1007/s00705-013-1960-8

    • NAID

      120005647478

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Rabies virus glycoprotein variants display different patterns in rabies monosynaptic tracing.2014

    • Author(s)
      Takuma Mori, Kinjiro Morimoto
    • Journal Title

      Frontiers in Neuroanatomy

      Volume: 7 Pages: 47-47

    • DOI

      10.3389/fnana.2013.00047

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] ウイルス吸着・侵入あるいはレクチン結合による細胞遺伝子の発現変動2014

    • Author(s)
      森本金次郎、佐藤雄一郎
    • Journal Title

      安田女子大学紀要

      Volume: 42 Pages: 329-336

    • Related Report
      2013 Research-status Report
  • [Journal Article] High Mannose-Binding Antiviral Lectin PFL from Pseudomonas fluorescens Pf0-1 Promotes Cell Death of Gastric Cancer Cell MKN28 via Interaction with 2-integrin.2012

    • Author(s)
      Yuichiro Sato
    • Journal Title

      PLOS ONE

      Volume: 7 Issue: 9 Pages: 45922-45922

    • DOI

      10.1371/journal.pone.0045922

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecular analysis of the mutational effects of Thai street rabies virus with increased virulence in mice after passages in the BHK cell line.2012

    • Author(s)
      P. Virojanapirom
    • Journal Title

      Archives of Virology

      Volume: 157 Issue: 11 Pages: 2201-2205

    • DOI

      10.1007/s00705-012-1402-z

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Serial passage of a street rabies virus in mouse neuroblastoma cells resulted in attenuation: potential role of the additional N-glycosylation of a viral glycoprotein in reduced pathogenicity of street rabies virus.2012

    • Author(s)
      Kentaro Yamada
    • Journal Title

      Virus Research

      Volume: 165 Pages: 34-45

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 鯉ヘルペスウイルスの感染抗原に対する鶏卵抗体(IgY)の調製2015

    • Author(s)
      八田一、杣本智軌、佐藤巧視、森本金次郎
    • Organizer
      日本農芸化学会 2015年度大会
    • Place of Presentation
      岡山大学津島キャンパス(岡山)
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 非増殖型組換え狂犬病ウイルスを用いたアレナウイルスに対するワクチンの開発2014

    • Author(s)
      伊藤(高山)睦代、林 昌宏、森本金次郎、垣内五月、山口幸恵、堀谷まどか、西條政幸
    • Organizer
      第62回 日本ウイルス学会学術集会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2014-11-10 – 2014-11-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] 細菌レクチンPFLの誘導するインテグリン/EGFRの内在化とオートファジー性がん細胞死2014

    • Author(s)
      佐藤雄一郎、森本金次郎、久保貴紀、瀬山敏雄
    • Organizer
      第87回 日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都)
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] リンパ球性脈絡髄膜炎ウイルス感染症に対する非増殖型組換え狂犬病ワクチンの開発2014

    • Author(s)
      西條政幸、伊藤(高山)睦代、森本金次郎、垣内五月、山口幸恵、堀谷まどか、林 昌宏
    • Organizer
      第19回 日本神経感染症学会総会学術集会
    • Place of Presentation
      金沢歌劇座(金沢)
    • Year and Date
      2014-09-04 – 2014-09-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] Preparation of anti-Koi Herpes Virus IgY and its prophylactic effect2014

    • Author(s)
      Hajime Hatta, Tomonori Somamoto, Atsushi Satou, Kinjiro Morimoto
    • Organizer
      EGG BANFF FORUM CONFERENCE
    • Place of Presentation
      Wroclow, Poland
    • Year and Date
      2014-06-25 – 2014-06-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] ラッサウイルスなどのアレナウイルスに対する非増殖型組換え狂犬病ウイルスワクチンの開発2013

    • Author(s)
      伊藤(高山)睦代
    • Organizer
      日本ウイルス学会学術集会
    • Place of Presentation
      神戸国際会議場(神戸)
    • Related Report
      2013 Research-status Report
  • [Presentation] 細菌レクチン(PFL)により誘導されるアノイキス様がん細胞死とオートファジーの関係2013

    • Author(s)
      佐藤 雄一郎
    • Organizer
      日本薬学会・日本薬剤師会・日本病院薬剤師会 中国四国支部学術大会
    • Place of Presentation
      松山大学(松山)
    • Related Report
      2013 Research-status Report
  • [Presentation] 細菌Pseudomonas fluorescens Pf0-1由来レクチン(PFL)の抗ウイルス、抗がん作用の分子機構2012

    • Author(s)
      佐藤 雄一郎
    • Organizer
      日本生化学会大会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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