| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Outline of Final Research Achievements |
An unusual alternative splicing occurring within the prion protein gene which resulted in the generation of mRNA lacking the GPI anchor signal sequence, GPI-anchorless PrP (PrPSV), was identified in the human, bovine and sheep cell lines. PrP or PrPSV cDNAs from sheep were cloned into the retroviral vector. To make sure that proper proteins are produced, HpL3-4 cells, murine PrP deficient cell line, were used. Stable expression of sheep PrP or PrPSV in the HpL3-4 cells were cultured with scrapie, and then the Proteinase K-resistant prion protein (PrPres) positive cells were detected. However, PrPres were detected in the mock-infected cells. The PrP- or PrPSV-expressing cell lines would be useful to investigate prion pathology.
|
