Uptake mechanisms study and molecular analysis of biomedicine in tissue culture system.
| Project/Area Number |
24590218
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Meiji Pharmaceutical University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 酵素補充療法 / マンノース６リン酸レセプター / バイオ医薬品 / 薬物動態 / 血管内皮細胞 |
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| Outline of Final Research Achievements |
To estimate uptake mechanism of biomedicine such as alpha-galactosidase (GLA), we developed the evaluation system of uptake analysis system using in vitro primary culture cell. We proved GLA uptake dependent mannose-6-phosphate receptor (M6PR) in primary cultured cell. However, M6PR independent uptake was obtained in renal proximal tubule epithelial cells and renal cortical epithelial cells. These results correlate with in vivo pharmacokinetics. These results suggest that our cell culture system is useful for pharmacokinetic study in pre-clinical.
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Report
(4 results)
Research Products
(8 results)
