| Project/Area Number |
24590237
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Yokohama City University |
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Principal Investigator |
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| Research Collaborator |
OHNO Shigeo
MORIYAMA Kayano
ASADA Kio
FUKUDA Ayano
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 細胞極性 / 平面極性 / 形態形成 / 遺伝子相互作用 / aPKC / Daam1 / Wnt-PCP / 発生学 / 形態形成学 / リン酸化プロテオミクス / ノックアウトマウス / 薬剤誘導性遺伝子ノックアウトシステム / 不死化上皮細胞株 / ES細胞株 / プロテオミクス解析 / 発生分化 |
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| Outline of Final Research Achievements |
Proper regulation of cell polarity and directional cell movements is essential to form correct three-dimensional architecture. Atypical protein kinase C (aPKC) is known as the key regulator for apical-basal (z axis) cell polarity, however, it is not clarified the role in the regulation of cell sheet (x-y plane) polarity, as well as planar cell polarity (PCP) in relation to Wnts and other signaling cascades. In this study, we show that aPKC genetically connect to Daam1 (Dshevelled associated activator of morphogenesis 1), which plays important role in Wnt-PCP signaling. We also found functional interaction between aPKC and Daam1 in Xenopus embryogenesis. Further, we present physical interaction between aPKC and Daam1. These results strongly suggest that aPKC contributes planar cell polarity regulation, in vivo and in vitro.
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