Establishment of the differentiation method to spermatogenesis by the xenogeneic transplantation of spermatogonia stem cells and the in vitro culture
Project/Area Number |
24590243
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General anatomy (including Histology/Embryology)
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Research Institution | Tokyo Medical University |
Principal Investigator |
Qu Ning 東京医科大学, 医学部, 講師 (70527952)
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Co-Investigator(Kenkyū-buntansha) |
ITOH Masahiro 東京医科大学, 医学部, 教授 (00232471)
YOSHIMOTO Takayuki 東京医科大学, 医学部, 教授 (80202406)
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Project Period (FY) |
2012-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | 精子形成 / 精子幹細胞 / 異種移植 / 免疫寛容 / マウス / 精巣 / マクロファージ / サイトカイン / 抗がん剤 / 移植 / 免疫抑制 |
Outline of Final Research Achievements |
There have been increasing reports on xenogeneic transplantation of mammalian male spermatogonial stem cells (SSCs). However, in most previous studies on xenogeneic SSCs transplantation, to avoid immunologic rejection, immunodeficient or immunosuppressed mice were used as the recipients, which were pre-treated with busulfan (Myleran, 1,4-butanediol methanesulfonate) for depletion of endogenous germ cells before the SSCs transplantation. This study demonstrated that in the testes of busulfan-treated immune-competent mice, the increase of TNF-a in the seminiferous tubule and the infiltration of the macrophage were seen in the interstitium, and the increase of apoptotic cytokine such as Fas,FasL,caspase8 and the decrease of caspase9 were detected. Furthermore, it was revealed that immune-suppressive cytokines and M2 macrophage in the testis are related with testicular immune privileged circumstances.
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Report
(5 results)
Research Products
(28 results)
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[Journal Article] Contribution of IL-12/IL-35 common subunit p35 to maintaining the testicular immune privilege2014
Author(s)
Terayama H, Yoshimoto T, Hirai S, Naito M, Qu N, Hatayama N, Hayashi S, Mitobe K, Furusawa J, Mizoguchi I, Kezuka T, Goto H, Suyama K, Moriyama H, Sakabe K, Itoh M.
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Journal Title
PLoS One
Volume: 9
Issue: 4
Pages: e96120-e96120
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Neonatal estrogen treatment with β-estradiol 17-cypionate induces in post-pubertal mice inflammation in the ductuli efferentes, epididymis, and vas deferens, but not in the testis, provoking obstructive azoospermia.2014
Author(s)
Naito M, Hirai S, Terayama H, Qu N, Hayashi S, Hatayama N, Kawamura H, Nakano T, Itoh M.
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Journal Title
Med Mol Morphol.
Volume: 47
Issue: 1
Pages: 21-30
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Pivotal Roles of T-Helper 17-Related Cytokines, IL-17, IL-22, and IL-23, in Inflammatory Diseases.2013
Author(s)
Qu N, Xu M, Mizoguchi I, Furusawa J, Kaneko K, Watanabe K, Mizuguchi J, Itoh M,Kawakami Y, Yoshimoto T.
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Journal Title
Clin Dev Immunol.
Volume: 2013:968549
Pages: 1-13
DOI
Related Report
Peer Reviewed
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[Journal Article] Cadmium exposure increases susceptibility to testicular autoimmunity in mice2013
Author(s)
Ogawa Y, Itoh M, Hirai S, Suna S, Naito M, Qu N, Terayama H, Ikeda A, Miyaso H, Matsuno Y, Komiyama M, Mori C
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Journal Title
J Appl Toxicol
Volume: 33(7)
Pages: 652-660
Related Report
Peer Reviewed
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[Journal Article] Effects on the local immunity in the testis by exposure to di-(2-ethylhexyl) phthalate (DEHP) in mice.2013
Author(s)
Kitaoka M, Hirai S, Terayama H, Naito M, Qu N, Hatayama N, Miyaso H, Matsuno Y, Komiyama M, Itoh M, Mori C
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Journal Title
J Reprod Dev
Volume: 59(5)
Pages: 485-490
Related Report
Peer Reviewed
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[Presentation] 精巣におけるマクロファージの免疫寛容機構2016
Author(s)
曲 寧,寺山 隼人, 平井 宗一, 林 省吾、畑山 直之,倉升 三幸, 小川 夕輝, 坂部 貢, 伊藤 正裕
Organizer
第121回日本解剖学会・全国学術集会
Place of Presentation
福島
Year and Date
2016-03-28
Related Report
Invited
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[Presentation] ラットにおける異所性精巣移植の試み2016
Author(s)
畑山 直之,曲 寧,林 省吾,平井 宗一,、倉升 三幸, 永堀 健太,小川 夕輝, 伊藤 正裕
Organizer
第121回日本解剖学会・全国学術集会
Place of Presentation
福島
Year and Date
2016-03-28
Related Report
Invited
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[Presentation] Mono-(2-ethylhexyl) phthalate on drug transporter, P-glycoprotein (MDR1), in the testis of prepubertal rats2014
Author(s)
内藤 宗和, 平井 宗一, 曲 寧, 林 省吾, 畑山 直之, 小川 夕輝, 倉升 三幸, 河田 晋一, 伊藤 正裕
Organizer
第119回日本解剖学会・全国学術集会
Place of Presentation
栃木
Related Report
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