Expression of ion-channel proteins in the cutaneous mechanoreceptors of normal rats and atopic dermatitis model mice
| Project/Area Number |
24590253
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Tottori University |
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Principal Investigator |
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| Research Collaborator |
KAMEIE Toshio 鳥取大学, 技術部, 技術専門職員
MORINO Shinichi 鳥取大学, 技術部, 技術専門職員
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 皮膚 / 感覚 / 神経 / アトピー性皮膚炎 / チャネル分子 / 感覚受容器 / 接着分子 / 機械受容器 |
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| Outline of Final Research Achievements |
Immunohistochemistry showed that ion-channel proteins, Piezo2, TRPV2, ASIC2, and ASIC3 were expressed in the palisade nerve endings（PN）of rat vellus hairs. These results means that the PN in this skin region might be function as not only mechanoreceptor but also thermoreceptor and nociceptor. Immunoelectron microscopy confirmed that ion-channel receptor proteins, Piezo2 and ASIC2 were expressed on the cytoplasmic surface of plasma membrane of the sensory nerve endings. These proteins probably function as receptors of mechanosensation of the lanceolate nerve endings in the rat auricular skin.
In the auricular skin of the normal NC-hairless mice the expression of ion-channel proteins was inconspicuous in the PN, whereas in that of the dinitrofluorobenzene-induced atopic dermatitis mice, TRPV4, TRPA1, ASIC2 and ASIC3 were detected in the PN. Induction and expression of these ion-channel proteins in PN might cause itchy skin sensation in this atopic dermatitis model.
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Report
(4 results)
Research Products
(5 results)
