| Project/Area Number |
24590265
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kurume University |
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Principal Investigator |
OHTA Keisuke 久留米大学, 医学部, 准教授 (00258401)
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| Co-Investigator(Kenkyū-buntansha) |
RIKIMARU Yukiko 久留米大学, 医学部, 助教 (90368960)
KOGA Noriyuki 久留米大学, 医学部, 講師 (30309851)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAMURA Kei-ichiro 久留米大学, 医学部, 教授 (20172398)
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| Research Collaborator |
OKA Takeshi
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 真皮 / 創傷治癒 / マクロファージ / 線維芽細胞 / FIB-SEM / 三次元再構築 / ストラクトーム / 皮膚 / 細胞間接着 / 細胞性ネットワーク / 皮膚病変 / ケロイド / 肥厚性瘢痕 / 細胞接着 |
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| Outline of Final Research Achievements |
In this study, we attempted to visualize detail spacial relationships between macrophages and fibroblasts in the dermis using recent advanced 3D reconstruction technic "FIB-SEM tomography". We showed the cells in normal skin frequently made contacts each other. The macrophage located near the fibroblast was wrapped by the cellular processes of the fibroblasts almost 1/3 of the total surface of it. On the other hand, the cells in the pathologic dermis tended to reduce the contact frequency than the normal dermis. Co-culture of fibroblast and macrophage also showed the close contacts between them, and promoted the gene expression which related to the intracellular signaling. These results suggest that the direct cell to cell contact between fibroblast and macrophage relates to some sort of tissue maintenance functions.
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