Functional analysis of acid-sensitive ion channels in mouse pituitary gland.
Project/Area Number |
24590281
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General physiology
|
Research Institution | Nagoya City University |
Principal Investigator |
UEDA Takashi 名古屋市立大学, 医学(系)研究科(研究院), 准教授 (90244540)
|
Co-Investigator(Kenkyū-buntansha) |
UGAWA Shinya 名古屋市立大学, 大学院医学研究科, 教授 (20326135)
|
Co-Investigator(Renkei-kenkyūsha) |
SHIMADA Shoichi 大阪大学, 大学院医学系研究科, 教授 (20216063)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
Keywords | 刺激分泌関連 / イオンチャネル型オーファン受容体 / 酸感受性イオンチャネル / 下垂体後葉 / 傍分泌 / ノックアウトマウス / レポーターマウス / 下垂体機能 / 下垂体 / 副腎髄質 / ASIC family / TRPV family |
Outline of Final Research Achievements |
In mouse pituitary gland, the anterior (AL) and intermediate lobes (IL) expressed acid-sensing ion channel 1(ASIC1) and ASIC4, whereas the posterior lobe (PL) expressed transient receptor potential vanilloid type 2 (TRPV2) channel. TRPV2, not TRPV4, was expressed in a unique subpopulation of glia-like pituicytes in the PL. Proton activated ASIC4 as well as ASIC1 in Xenopus oocytes. A TRPV2 agonist probenecid elevated the intracellular calcium concentration in a subset of the isolated pituitary gland cells (approximately 12 % of total cells) and evoked ATP release in these cells. Since ATP is known to be a paracrine modulator of vasopressin release in the PL, TRPV2 may be involved in the regulation of the endocrine system.
|
Report
(4 results)
Research Products
(7 results)
-
-
-
[Presentation] マウス鼻腔上皮におけるTRPV4チャネルの機能発現2015
Author(s)
植田高史、星川真理子、柴田泰宏、熊本奈都子、鵜川眞也
Organizer
第120回日本解剖学会総会・全国学術集会、第92回日本生理学会大会、合同学会
Place of Presentation
神戸国際会議場・ 展示場( 神戸ポートアイランド )( 兵庫県・ 神戸市)
Year and Date
2015-03-21 – 2015-03-23
Related Report
-
-
-
-