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Roles of afadin and nectins in synapse formation

Research Project

Project/Area Number 24590354
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General medical chemistry
Research InstitutionKobe University

Principal Investigator

MANDAI Kenji  神戸大学, 医学(系)研究科(研究院), 准教授 (50322186)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsシナプス形成 / アファディン / ネクチン / 海馬 / 大脳新皮質 / 中脳水道 / 嗅球 / 分子神経生物学 / シナプス
Outline of Final Research Achievements

We investigated the roles of afadin in synapse formation and synaptic functions in the hippocampus. At mossy fiber-CA3 pyramidal cell synapses, afadin was required for the accumulation of the PAJ components, nectin-1, nectin-3 and N-cadherin, and for the accumulation of the presynaptic components, VGLUT1 and bassoon. In addition, afadin regulated pre- and post-synaptic functions in cultured hippocampal excitatory neurons. We also investigated the roles of afadin in the developing brain. In the midbrain, afadin was required for morphogenesis of the cerebral aqueduct, and in the neocortex, afadin was required for the formation of the six-layered laminated architecture by regulating neural migration. Furthermore, we investigated the roles of nectin-1 in the olfactory bulb and showed that nectin-1 constituted a novel adhesion apparatus that tethered mitral cell dendrites in a dendritic meshwork structure of the developing mouse olfactory bulb.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (14 results)

All 2015 2014 2013 2012 Other

All Journal Article (9 results) (of which Peer Reviewed: 8 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (5 results)

  • [Journal Article] Nectins and nectin-like molecules in development and disease.2015

    • Author(s)
      Mandai K, Rikitake Y, Mori M, Takai Y.
    • Journal Title

      Curr Top Dev Biol.

      Volume: 112 Pages: 197-231

    • DOI

      10.1016/bs.ctdb.2014.11.019

    • ISBN
      9780124077584
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Nectin-1 spots as a novel adhesion apparatus that tethers mitral cell lateral dendrites in a dendritic meshwork structure of the developing mouse olfactory bulb.2015

    • Author(s)
      Inoue, T., Fujiwara, T., Rikitake, Y., Maruo, T., Mandai, K., Kimura, K., Kayahara, T., Wang, S., Ioth, Y., Sai, K., Mori, M., Mori, K., Mizoguchi, A., & Takai, Y.
    • Journal Title

      J Comp Neurol.

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impairment of radial glial scaffold-dependent neuronal migration and formation of double cortex by genetic ablation of afadin.2015

    • Author(s)
      Yamamoto, H., Mandai, K., Konno, D., Maruo, T., Matsuzaki, F., & Takai, Y.
    • Journal Title

      Brain Res.

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Roles of nectins and nectin-like molecules in the nervous system.2014

    • Author(s)
      Mori, M., Rikitake, Y., Mandai, K., & Takai, Y.
    • Journal Title

      Adv Neurobiol.

      Volume: 8 Pages: 91-116

    • Related Report
      2014 Annual Research Report
  • [Journal Article] s-Afadin binds more preferentially to the cell adhesion molecules nectins than l-afadin.2014

    • Author(s)
      Kobayashi R, Kurita S, Miyata M, Maruo T, Mandai K, Rikitake Y, Takai Y.
    • Journal Title

      Genes Cells.

      Volume: 19 Issue: 12 Pages: 853-863

    • DOI

      10.1111/gtc.12185

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Afadin regulates puncta adherentia junction formation and presynaptic differentiation in hippocampal neurons.2014

    • Author(s)
      Toyoshima D, Mandai K, Maruo T, Supriyanto I, Togashi H, Inoue T, Mori M, Takai Y.
    • Journal Title

      PLoS One.

      Volume: 9 Issue: 2 Pages: e89763-e89763

    • DOI

      10.1371/journal.pone.0089763

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Afadin/AF-6 and Canoe: Roles in cell adhesion and beyond2013

    • Author(s)
      Kenji Mandai
    • Journal Title

      Progress in Molecular Biology and Translational Science

      Volume: 116 Pages: 433-454

    • DOI

      10.1016/b978-0-12-394311-8.00019-4

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] The role of nectins in different types of cell-cell adhesion.2012

    • Author(s)
      Rikitake, Y.
    • Journal Title

      J Cell Sci.

      Volume: 125 Issue: 16 Pages: 3713-3722

    • DOI

      10.1242/jcs.099572

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Immunoglobulin superfamily receptors and adherens junctions.2012

    • Author(s)
      Shimono, Y.
    • Journal Title

      Subcell Biochem.

      Volume: 60 Pages: 137-170

    • DOI

      10.1007/978-94-007-4186-7_7

    • ISBN
      9789400741850, 9789400741867
    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] SBF-SEM 3D reconstitution analysis reveals alterations in composition and morphology of mouse hippocampal mossy fiber synapses by afadin knockout.2015

    • Author(s)
      Fujiwara, T., Wang, S., Itoh, Y., Sai, K., Kaito, A., Miyazaki, N., Murata, K., Maruo, T., Yamamoto, H., Mandai, K., Takai, Y., & Mizoguchi, A.
    • Organizer
      第120回日本解剖学会全国学術集会・第92回日本生理学会大会合同大会
    • Place of Presentation
      神戸国際会議場・展示場(兵庫県神戸市)
    • Year and Date
      2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Afadin plays an important role in formation of puncta adherentia junction and differentiation of presynapses in hippocampal neurons.2014

    • Author(s)
      Toyoshima, D., Mandai, K., Maruo, T., Supriyanto, I., Togashi, H., Inoue, T., Mori, M., & Takai, Y.
    • Organizer
      Society for Neuroscience, 2014 Annual Meeting
    • Place of Presentation
      Washington DC, U.S.A.
    • Year and Date
      2014-11-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Pathophysiological roles of afadin in the brain development.2014

    • Author(s)
      Yamamoto, H., Mandai, K., Maruo, T., Konno, D., & Matsuzaki, F.
    • Organizer
      第37回日本神経科学会大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Abnormal radial glial scaffold for migrating neurons leads to formation of the double cortex in afadin-deficient mice.2014

    • Author(s)
      Yamamoto, H., Mandai, K., Konno, D., Maruo, T., Matsuzaki, F., & Takai, Y.
    • Organizer
      Cold Spring Harbor Laboratory Meeting, Glia in Health & Disease
    • Place of Presentation
      Cold Spring Harbor, NY, U.S.A.
    • Year and Date
      2014-07-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] Afadin is involved in synaptogenesis of hippocampal neurons.

    • Author(s)
      Maruo, T., Toyoshima, D., Mandai, K., Togashi, H., Inoue, T., Yamamoto, H., Miyoshi, J., Supriyanto, I., Mori, M., and Takai, Y.
    • Organizer
      第36回日本神経科学会大会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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