Functional analysis of Fanconi anemia proteins in chromosome fragile site

• Project/Area Number: 24590391
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: MATSUSHITA Nobuko 東京薬科大学, 生命科学部, 准教授 (30333222)
• Co-Investigator(Kenkyū-buntansha): YANAGI Shigeru 東京薬科大学, 生命科学部, 教授 (60252003)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: DNA複製 / ファンコニ貧血 / DNA損傷修復

Outline of Final Research Achievements

We identified several genes that are involved in DNA replication stress response by siRNA library screening. These genes are reported to function as chromatin remodeling factors, but their roles in replication stress response are not clear. One of them is FancD2 related protein (D2RP) which interacts with FANCD2. According to the functional analysis, we identified that D2RP involved in DNA damage response. However, D2RP dose not take a role in FancD2 localization following DNA replication stress.

Research Products

• [Journal Article] Modularized functions of the Fanconi anemia core complex. (2014)
  • Author(s): Huang Y, Leung JW, Lowery M, Matsushita N, Wang Y, Shen X, Huong D, Takata M, Chen J, Li L.
  • Journal Title: Cell Rep. Volume: 7 Issue: 6 Pages: 1849-57
  • DOI: 10.1016/j.celrep.2014.04.029
  • Peer Reviewed / Open Access
• [Journal Article] MITOL regulates endoplasmic reticulum-mitochondria contacts via Mitofusin2. (2013)
  • Author(s): Sugiura, A., Nagashima, S., Tokuyama, T., Amo, T., Matsuki, Y., Ishido, S., Kudo, Y., McBride, H.M., Fukuda, T., Matsushita, T., Inatome, R., and C Yanagi, S.
  • Journal Title: Mol. Cell Volume: 51 Issue: 1 Pages: 1-15
  • DOI: 10.1016/j.molcel.2013.04.023
  • Peer Reviewed
• [Presentation] TAX1BP1 regulates B cell differentiation through NF-κB pathway (2014)
  • Author(s): 鈴木みどり、伊波英克、柳 茂、松下暢子
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-26
• [Presentation] TAX1BP1は NF-κB経路を抑制化することによって特異抗体産生を制御する (2014)
  • Author(s): 鈴木みどり、伊波英克、柳 茂、松下暢子
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-18
• [Presentation] TAX1BP1 modulates antigen-specific antibody response through NF-κB pathway (2014)
  • Author(s): 鈴木みどり、伊波英克、柳 茂、松下暢子
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] TAX1BP1は体細胞高頻度突然変異を制御することによって抗体遺伝子多様化機構に機能する (2013)
  • Author(s): 松下暢子、鈴木みどり、伊波英克、柳 茂
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] TAX1BP1 regulates IgM expression at the cell surface through NF-κB pathway (2013)
  • Author(s): 鈴木みどり、松下暢子、伊波英克、柳 茂
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] TAX1BP1 plays a role in immunoglobulin gene diversification through regulation of somatic hyper mutation (2013)
  • Author(s): 松下暢子、鈴木みどり、柳 茂.
  • Organizer: 第36回 日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド