Regulation mechanisms by micro-RNA in scirrhous carcinoma
| Project/Area Number |
24590431
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KURODA Masahiko 東京医科大学, 医学部, 教授 (80251304)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 胃癌 / マイクロRNA / 上皮間葉移行 / miR-200c / Zeb1 / Zeb2 / E-cadherin / tissue array / tisssue array |
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| Outline of Final Research Achievements |
Poorly differentiated gastric carcinoma usually presents as scirrhous carcinoma, which is highly proliferative with poor prognosis. Concerning invasive ability and morphology of gastric carcinoma, micro-RNA (miR)-200c, which is associated with epithelial-mesenchymal transition, was investigated. In situ hybridization of gastric cancer tissue revealed that expression of miR-200c was enhanced in well differentiated adenocarcinomas, while its expression was decreased in poorly differentiated adenocarcinomas. ZEB1, ZEB2, and E-cadherin, the targets of miR-200c, also varied in accordance with expression level of miR-200c. Investigation of cell lines showed that expression of miR-200c was preserved in rounded cells, while it was down-regulated in spindle cells, with paralleled shifts of the target RNAs and proteins. It was suggested that down-regulation of miR-200c plays a role in epithelial-mesenchymal transition in gastric carcinoma.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Correlation between number of renal cysts and aortic circumferences measured using autopsy material.2013
Author(s)
Kurata A, Inoue S, Ohno S, Nakatsubo R, Takahashi K, Ito T, Kawasaki K, Kokubo R, Sakai T, Ubukata J, Matsubara S, Muraoka R, Yamazaki J, Hirose T, Hojo M, Watanabe E, Kuroda M.
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Journal Title
Pathol Res Pract
Volume: 209
Issue: 7
Pages: 441-447
DOI
Related Report
Peer Reviewed
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[Journal Article] AP-VAS 2012 case report: a case of systemic MPO-ANCA-associated vasculitis that demonstrated brain infarction and immunohistochemically MPO-positive capillaries.2013
Author(s)
Kurata A, Kawashima S, Terado Y, Komagata Y, Kaname S, Okano H, Nishiyama K, Hirano K, Kamma H, Arimura Y, Yamada A.
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Journal Title
CEN Case Rep
Volume: 2
Issue: 2
Pages: 148-153
DOI
Related Report
Peer Reviewed
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