| Project/Area Number |
24590431
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KURODA Masahiko 東京医科大学, 医学部, 教授 (80251304)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 胃癌 / マイクロRNA / 上皮間葉移行 / miR-200c / Zeb1 / Zeb2 / E-cadherin / tissue array / tisssue array |
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| Outline of Final Research Achievements |
Poorly differentiated gastric carcinoma usually presents as scirrhous carcinoma, which is highly proliferative with poor prognosis. Concerning invasive ability and morphology of gastric carcinoma, micro-RNA (miR)-200c, which is associated with epithelial-mesenchymal transition, was investigated. In situ hybridization of gastric cancer tissue revealed that expression of miR-200c was enhanced in well differentiated adenocarcinomas, while its expression was decreased in poorly differentiated adenocarcinomas. ZEB1, ZEB2, and E-cadherin, the targets of miR-200c, also varied in accordance with expression level of miR-200c. Investigation of cell lines showed that expression of miR-200c was preserved in rounded cells, while it was down-regulated in spindle cells, with paralleled shifts of the target RNAs and proteins. It was suggested that down-regulation of miR-200c plays a role in epithelial-mesenchymal transition in gastric carcinoma.
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