| Project/Area Number |
24590433
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
ESUMI Mariko 日本大学, 医学部, 准教授 (10147019)
TOU Shoen 日本大学, 医学部, 助教 (20326036)
FUCHINOUE Fumi 日本大学, 医学部, 助手 (10409021)
AMANO Sadao 日本大学, 医学部, 准教授 (80159459)
SAKURAI Kenichi 日本大学, 医学部, 講師 (20366602)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 乳癌 / 遺伝子変異多様性 / クロナリティ / ミトコンドリアDNA D-loop領域 / ミトコンドリアDNA / 癌細胞系譜 / クロナリティー / 細胞系譜 / 再発 |
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| Outline of Final Research Achievements |
(1)Gene alteration of mtDNA-DL (GAMDDL) was detected in metachronously resected ipsilateral normal breast (0.03%)and contralateral breast (0.74%).(2)Breast cancers with polysomy of centromere 17 and those with HER2 gene heterogeneity were not overlapped. (3)The protocol to visualize HER2 protein and gene simultaneously was established.(4)Primary breast cancer with heterogeneity, e.g. with and without HER2 gene amplification, and recurrent tumor without HER2 gene amplification was analyzed by GAMDDL method. There are differences of MDDL between carcinoma cells of primary tumor with and without HER2 gene amplification.
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