An analysis of cell lineage for breast cancer by gene alteration of mitochondria DNA D-loop region

• Project/Area Number: 24590433
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Nihon University
• Principal Investigator: MASUDA Shinobu 日本大学, 医学部, 教授 (20276794)
• Co-Investigator(Renkei-kenkyūsha): ESUMI Mariko 日本大学, 医学部, 准教授 (10147019) ; TOU Shoen 日本大学, 医学部, 助教 (20326036) ; FUCHINOUE Fumi 日本大学, 医学部, 助手 (10409021) ; AMANO Sadao 日本大学, 医学部, 准教授 (80159459) ; SAKURAI Kenichi 日本大学, 医学部, 講師 (20366602)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 乳癌 / 遺伝子変異多様性 / クロナリティ / ミトコンドリアDNA D-loop領域 / ミトコンドリアDNA / 癌細胞系譜 / クロナリティー / 細胞系譜 / 再発

Outline of Final Research Achievements

(1)Gene alteration of mtDNA-DL (GAMDDL) was detected in metachronously resected ipsilateral normal breast (0.03%)and contralateral breast (0.74%).(2)Breast cancers with polysomy of centromere 17 and those with HER2 gene heterogeneity were not overlapped. (3)The protocol to visualize HER2 protein and gene simultaneously was established.(4)Primary breast cancer with heterogeneity, e.g. with and without HER2 gene amplification, and recurrent tumor without HER2 gene amplification was analyzed by GAMDDL method. There are differences of MDDL between carcinoma cells of primary tumor with and without HER2 gene amplification.

Research Products

• [Journal Article] Histopathological characteristics of breast ductal carcinoma in situ and association with imaging findings. (2015)
  • Author(s): Tang X, Yamashita T, Hara M, Kumaki N, Tokuda Y, Masuda S.
  • Journal Title: Breast Cancer Volume: 印刷中 Issue: 3 Pages: 491-498
  • DOI: 10.1007/s12282-015-0592-0
  • Peer Reviewed
• [Journal Article] Pathological examination of breast cancer biomarkers: current status in Japan. (2015)
  • Author(s): Masuda S
  • Journal Title: Breast Cancer Volume: 印刷中 Issue: 4 Pages: 485-494
  • DOI: 10.1007/s12282-014-0566-7
  • Peer Reviewed
• [Journal Article] Altered intracellular region of MUC1 and disrupted correlation of polarity-related molecules in breast cancer subtypes. (2015)
  • Author(s): Iizuka M, Nakanishi Y, Fuchinoue F, Maeda T, Murakami E, Obana Y, Enomoto K, Tani M, Sakurai K, Amano S, Masuda S.
  • Journal Title: Cancer Science Volume: 106 Issue: 3 Pages: 307-314
  • DOI: 10.1111/cas.12596
  • Peer Reviewed / Open Access
• [Journal Article] Overexpression of PGC1α and accumulation of p62 in apocrine carcinoma of the breast. (2015)
  • Author(s): Fuchinoue F, Hirotani Y, Nakanishi Y, Yamaguchi H, Nishimaki H, Noda H, Tang X, Iiuzuka M, Amano S, Sugitani M, Nemoto N, Masuda S.
  • Journal Title: Patholology International Volume: 65 Issue: 1 Pages: 19-26
  • DOI: 10.1111/pin.12235
  • Peer Reviewed
• [Journal Article] Comparative Study of the One-step Nucleic Acid Amplification Assay and Conventional Histlogical Examination for the Detection of Breast Cancer Sentinel Lymph Node Metastases. (2014)
  • Author(s): Terada M, Niikura N, Tsuda B, Masuda S, Kumaki N, Tang X, Okamura T, Saito Y, Suzuki Y, Tokuda Y.
  • Journal Title: Tokai Exp Clin Med Volume: 39 Pages: 122-127
  • Peer Reviewed
• [Journal Article] The cell cycle profiling-risk score based on CDK1 and 2 predicts early recurrence in node-negative, hormone receptor-positive breast cancer treated with endocrine therapy. (2014)
  • Author(s): Kim SJ, Masuda N, Tsukamoto F, Inaji H, Akiyama F, Sonoo H, Kurebayashi J, Yoshidome K, Tsujimoto M, Takei H, Masuda S, Nakamura S, Noguchi S.
  • Journal Title: Cancer Lett. Volume: 355 Issue: 2 Pages: 217-223
  • DOI: 10.1016/j.canlet.2014.08.042
  • Peer Reviewed / Open Access
• [Journal Article] Interpretation of HER2 tests in gastric cancer: confirmation of interobserver differences and validation of a QA/QC educational program. (2014)
  • Author(s): Kushima R, Kuwata T, Yao T, Kuriki H, Hashizume K, Masuda S, Tuda H, Ochiai A.
  • Journal Title: Virchows Arch Volume: 464 Issue: 5 Pages: 539-545
  • DOI: 10.1007/s00428-014-1567-9
  • Peer Reviewed
• [Journal Article] Prognostic Significance of the Ki67 Scoring Categories in Breast Cancer Subgroups. (2014)
  • Author(s): Niikura N, Masuda S, Kumaki N, Tang X, Terada M, Terao M, Iwamoto T, Oshitanai R, Morioka T, Tuda B, Okamura T, Saito Y, Suzuki Y, Tokuda Y.
  • Journal Title: Clinical Breast Cancer Volume: 14 Issue: 5 Pages: 323-329
  • DOI: 10.1016/j.clbc.2013.12.013
  • Peer Reviewed
• [Journal Article] Differing deregulation of HER2 in primary gastric cancer and synchronous related metastatic lymph nodes (2013)
  • Author(s): Kochi M, Masuda S, et al.
  • Journal Title: Diagnostic Pathology Volume: 8 Issue: 1 Pages: 191-191
  • DOI: 10.1186/1746-1596-8-191
  • Peer Reviewed
• [Journal Article] Analysis of gene alterations of mitochondrial DNA D-loop regions to determine breast cancer clonality. (2012)
  • Author(s): Masuda S, Kadowaki T, Kumaki N, Tang X, Tokuda Y, Yoshimura S, Takekoshi S, Osamura RY.
  • Journal Title: Br J Cancer Volume: 107 Issue: 12 Pages: 2016-23
  • DOI: 10.1038/bjc.2012.505
  • Peer Reviewed
• [Journal Article] Immunohistochemical Ki67 labeling index has a similar proliferation predictive power as various gene signatures in breast cancer. (2012)
  • Author(s): Niikura N, Iwamoto T, Masuda S, Kumaki N, Tang X, Shirane M, Mori K, Tsuda B, Okamura T, Saito Y, Suzuki Y, Tokuda Y.
  • Journal Title: Cancer Sci Volume: 103 Issue: 8 Pages: 1508-12
  • DOI: 10.1111/j.1349-7006.2012.02319.x
  • Peer Reviewed