The immunological analysis of MARCH I regulating IgE production

• Project/Area Number: 24590466
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Gihu University of Medical Science (2013-2015); The Institute of Physical and Chemical Research (2012)
• Principal Investigator: HOSHINO Mari 岐阜医療科学大学, 保健科学部, 教授 (10313511)
• Research Collaborator: HOSHINO Katsuaki 香川大学, 医学部, 教授 (50324843)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: E3ユビキチンリガーゼ / IgE / ユビキチンリガーゼ / MHCクラスII

Outline of Final Research Achievements

Membrane-associated RING-CH I (MARCH-I) has been suggested as a E3 ubiquitin ligase for the regulation of immune system.
In this study we observed lower antigen(Ag)-specific antibody responses, especially and drastically lower Ag-specific IgE responses and lower Ag-specific T cell responses when MARCH I deficient mice were systemically immunized, compared with control mice. Next, we showed that MARCH I deficiency in dendritic cells was important for the decrease of immune responses such as low IgE production. This study revealed that MARCH I might be responsible for susceptibility to infection.

Research Products

• [Int'l Joint Research] Medical School of Nanjing University/China Agricultural University(中国)
• [Journal Article] 樹状細胞の生存維持とMHCクラスIIのユビキチン化 (2013)
  • Author(s): 星野 真理
  • Journal Title: 臨床免疫・アレルギー科 Volume: 59(4) Pages: 491-9
• [Journal Article] HSP90α deficiency does not affect Ig gene hypermutation and class switch but causes enhanced MHC class II antigen presentation (2012)
  • Author(s): Li, Y., Li, S., Hoshino, M., Ishikawa, R., Kajiwara, C., Gao, X., Zhao, Y., Ishido, S., Udono, H., Wang, J. -Y.
  • Journal Title: Int. Immunol. Volume: 24 Issue: 12 Pages: 751-758
  • DOI: 10.1093/intimm/dxs076
  • Peer Reviewed
• [Presentation] MHC ubiquitination in dendritic cells is necessary for the normal immune responses (2014)
  • Author(s): Ｈｏｓｈｉｎｏ Ｍａｒｉ
  • Organizer: The 43rd Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 京都
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] Systemic immune responses in MARCH-I-deficient mice. (2013)
  • Author(s): M. Ohmura-Hoshino, S. Ishido
  • Organizer: 第42回日本免疫学会
  • Place of Presentation: 幕張メッセ
• [Presentation] High antigen-specific IgE production in MARCH-8 transgenic mice. (2012)
  • Author(s): M. Ohmura-Hoshino
  • Organizer: 第41回日本免疫学会
  • Place of Presentation: 神戸国際会議場(兵庫県神戸市)