Analyses of enteric bacterial products as a causative agent for the onset of intestinal amoebiasis

• Project/Area Number: 24590511
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Keio University
• Principal Investigator: KOBAYASHI Seiki 慶應義塾大学, 医学部, 講師 (70112688)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 赤痢アメーバ / Bacteroides fragilis / 腸持続感染 / GalGalNAcレクチン / ペルオキシダーゼ / ミエロペルオキシダーゼ / CBA マウス / 腸持続感染モデル / Tritrichomonas foetus / Gal/GalAc lectin / B. fragilis 培養上清 / 増殖促進因子 / Entamoeba dispar / マウス腸持続感染モデル / virulence / 糖鎖 / Citrobacter freundii

Outline of Final Research Achievements

Promoting effects for the growth of Entamoeba histolytica, and for both persistent infections of E. histolytica and Tritrichomonas foetus to the cecum of CBA mouse were found in the culture supernatant of obligate anaerobic Bacteroides fragilis. Increase of corresponding sugar chains for GalGalNAc lectin of E. histolytica and inhibition of peroxidase activity were found by analyses of the culture supernatant. These bacterial metabolites have a possibility to affect to the onset of amoebiasis that has various conditions from latent to fulminant type.

Research Products

• [Journal Article] Characterization of a human isolate of <i>Tritrichomonas foetus</i> (cattle/swine genotype) infected by a zoonotic opportunistic infection (2016)
  • Author(s): Suzuki J, Kobayashi S, Osuka H, Kawahata D, Oishi T, Sekiguchi K, Hamada A, Iwata S.
  • Journal Title: Journal of Veterinary Medical Science Volume: 78 Issue: 4 Pages: 633-640
  • DOI: 10.1292/jvms.15-0644
  • NAID: 130007331495
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Mouse model of amoebiasis and culture methods of amoeba (2016)
  • Author(s): Deloer S, Nakamura R, Mi-ichi F, Adachi K, Kobayashi S, Hamano S.
  • Journal Title: Parasitol. Int. Volume: in press Issue: 5 Pages: 30044-30047
  • DOI: 10.1016/j.parint.2016.03.012
  • Peer Reviewed / Open Access
• [Journal Article] Identification of Entamoeba polecki with unique 18S rRNA gene sequences from Celebes crested macaques and pigs in Tangkoko Nature Reserve, North Sulawesi, Indonesia. (2016)
  • Author(s): Tuda, J., Feng, M., Imada, M., Kobayashi, S., Cheng, X. and Tachibana, H.
  • Journal Title: J. Euk. Microbiol. Volume: 63 Issue: 5 Pages: 1-6
  • DOI: 10.1111/jeu.12304
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A case of peristomal cutaneous ulcer following amebic colitis caused by Entamoeba histolytica (2016)
  • Author(s): Sasaki Y, Yoshida T, Suzuki J, Kobayashi S, Sato T.
  • Journal Title: kansenshogaku Zasshi Volume: 90 Pages: 73-76
  • Peer Reviewed
• [Journal Article] Isolation and molecular characterization of Entamoeba nuttalli strains showing novel isoenzyme patterns from wild toque macaques in Sri Lanka (2016)
  • Author(s): Tachibana H, Yanagi T, Feng M, Bandara KB, Kobayashi S, Cheng X, Hirayama K, Rajapakse RP.
  • Journal Title: Journal of Eukaryotic Microbiology Volume: 63 Issue: 2 Pages: 171-180
  • DOI: 10.1111/jeu.12265
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 劇症型アメーバ症の診断および治療 (2015)
  • Author(s): 小林正規, 柳川泰昭, 渡辺恒二
  • Journal Title: 日本集中治療医学会雑誌 Volume: 22
  • NAID: 130005067812
  • Peer Reviewed
• [Journal Article] 赤痢アメーバの鑑別ポイントは？ (2014)
  • Author(s): 小林正規
  • Journal Title: Medical Technology Volume: 42 Pages: 828-829
• [Journal Article] 赤痢アメーバ (Entamoeba histolytica) (2014)
  • Author(s): 小林正規
  • Journal Title: 臨床検査 Volume: 58 Pages: 1427-1429
• [Journal Article] Species-specific immunity induced by infection with Entamoeba histolytica and Entamoeba moshkovskii in mice (2013)
  • Author(s): Shimokawa, C., Culleton, R., Imai, T., Suzue, K., Hirai, M., Taniguchi, T., Kobayashi, S., Hisaeda, H., Hamano, S
  • Journal Title: PLoS One Volume: 8(11) Issue: 11 Pages: e82025-e82025
  • DOI: 10.1371/journal.pone.0082025
  • NAID: 120006986684
  • Peer Reviewed
• [Journal Article] Prevalence of Entamoeba nuttalli infection in wild rhesus macaques in Nepal and characterization of the parasite isolates (2013)
  • Author(s): Tachibana H, Yanagi T, Lama C, Pandey K, Feng M, Kobayashi S, Sherchand JB
  • Journal Title: Parasitology International Volume: 62 Pages: 230-235
  • Peer Reviewed
• [Journal Article] Expression analysis of Entamoeba invadens profilins in encystation and excystation (2012)
  • Author(s): Makioka A, Kumagai M, Hiranuka K, Kobayashi S, Takeuchi T
  • Journal Title: Parasitology Research Volume: 110 Pages: 2095-2104
  • Peer Reviewed
• [Journal Article] Entamoeba moshkovskii is associated with diarrhea in infants and causes diarrhea and colitis in mice (2012)
  • Author(s): Shimokawa C, Kabir M, Taniuchi M, Mondal D, Kobayashi S, Ali IK, Sobuz SU, Senba M, Houpt E, Haque R, Petri WA Jr, Hamano S
  • Journal Title: Infectious Diseases Volume: 206 Pages: 744-751
  • Peer Reviewed
• [Presentation] ミャンマーに生息するアカゲザルからのEntamoeba nuttalliの分離とその遺伝子多型解析 (2015)
  • Author(s): 橘 裕司 、馮 萌 、Kosuwin Rattiporn、小林 正規 、柳 哲雄 、Mon Hla Myat 、 Putaporntip Chaturong 、Jongwutiwes Somchai
  • Organizer: 第84回日本寄生虫学会大会
  • Place of Presentation: 東京都三鷹市 杏林大学三鷹キャンパス
  • Year and Date: 2015-03-20 – 2015-03-22
• [Presentation] タイ中央部における野生カニクイザルからの Entamoeba nuttalli の分離とその性状解析 (2013)
  • Author(s): 橘 裕司， 柳 哲雄，王 女亭，河田 寿子，馮 萌，小林 正規，平山 謙二，Pattanawong Urassaya，Putaporntip Chaturong，Jongwutiwes Somchai
  • Organizer: 第82回日本寄生虫学会
  • Place of Presentation: 東京医科歯科大学
• [Presentation] Double-edged effects of IFN-γ in amoeba infection of mice (2013)
  • Author(s): 下川 周子，小林 正規，千馬 正敬，鈴江 一友，平井 誠，今井 孝，谷口 委
  • Organizer: 第82回日本寄生虫学会
  • Place of Presentation: 東京医科歯科大学
• [Book] 寄生虫学研究: 材料と方法 2014年度版： 赤痢アメーバ分離培養株の樹立 (2015)
  • Author(s): 小林正規, 津久井久美子，野崎智義
  • Total Pages: 3
  • Publisher: 三恵社