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Molecular and celluler signaling of hypoxic adaptation in the pathogenic yeast Cryptococcus neoformans

Research Project

Project/Area Number 24590518
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionChiba University

Principal Investigator

KAWAMOTO Susumu  千葉大学, 真菌医学研究センター, 教授 (80125921)

Co-Investigator(Renkei-kenkyūsha) HAGIWARA Daisuke  千葉大学, 真菌医学研究センター, 特任助教 (20612203)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords真菌 / クリプトコックス / 感染症 / 細胞周期 / 低酸素応答 / 環境適応 / G2-アレスト / 転写因子 / 低酸素ストレス / 病原真菌 / シグナル伝達 / 環境応答 / 細胞周期制御 / アグロバクテリウム / カルシニューリン
Outline of Final Research Achievements

Limited aeration has been demonstrated to cause slowdown in proliferation and delayed budding, resulting eventually in a unique unbudded G2-arrest in the obligate aerobic pathogenic yeast Cryptococcus neoformans. Also, the ability to adapt to decreased oxygen levels during pathogenesis has been identified as a virulence factor in C. neoformans. The aim of this study was to identify and characterize genes that are necessary for the proliferation slowdown and G2-arrest caused by limited aeration.  This gene was identified as homologous both to calcineurin responsive (Crz1) and PKC1-dependent (Sp1-like) transcription factors. Our data demonstrate a tight molecular link between slowdown of proliferation during hypoxic adaptation and maintenance of cell integrity in C. neoformans and present a new role for the Crz1 family of transcription factors in fungi.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (24 results)

All 2015 2014 2013 2012

All Journal Article (10 results) (of which Peer Reviewed: 10 results,  Open Access: 2 results) Presentation (14 results) (of which Invited: 6 results)

  • [Journal Article] Positional cloning in Cryptococcus neoformans and its application for identification and cloning of the gene encoding methylenetetrahydrofolate reductase.2015

    • Author(s)
      Akio Toh-e, Misako Ohkusu, Kiminori Shimizu, Susumu Kawamoto
    • Journal Title

      Fung. Genet. Biol.

      Volume: 印刷中

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The effects of F-actin inhibitor latrunculin A on pathogenic yeast Cryptococcus neoformans.2015

    • Author(s)
      Marie Kopecka, Masashi Yamaguchi, Susumu Kawamoto
    • Journal Title

      Chemotherapy

      Volume: 印刷中

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dendritic cell-based immunization ameliorates pulmonary infection with highly virulent Cryptococcus gattii.2015

    • Author(s)
      Keigo Ueno, Yuki Kinjo, Yoichiro Okubo, Kyoko Aki, Makoto Urai, Yukihiro Kaneko, Kiminori Shimizu, Dan-Ni Wang, Akiko Okawara, Takuya Nara, Kayo Ohkouchi, Yuki Mizuguchi, Susumu Kawamoto, Katsuhiko Kamei, Hideaki Ohno, Yoshihito Niki, Kazutoshi Shibuya, Yoshitsugu Miyazaki
    • Journal Title

      Infect. Immun.

      Volume: 83 Issue: 4 Pages: 1577-1586

    • DOI

      10.1128/iai.02827-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Functional characterization of PMT2, encoding a protein-O-mannosyltransferase, in the human pathogen Cryptococcus neoformans.2014

    • Author(s)
      Kiminori Shimizu, Yumi Imanishi, Akio Toh-e, Jun Uno, Hiroji Chibana, Christina M. Hull, Susumu. Kawamoto
    • Journal Title

      Fung. Genet. Biol.

      Volume: 69 Pages: 13-22

    • DOI

      10.1016/j.fgb.2014.05.007

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure based functional distinction between Cln1 and Cln2 depends on the ubiquitin-proteasome pathway.2014

    • Author(s)
      Akiko Suganami, Norio Takase, Hajime Sugiyama, Eric V. Virtudazo, Susumu Kawamoto, Yutaka Tamura
    • Journal Title

      J. Proteomics Bioinformatics

      Volume: 7 Issue: 05 Pages: 102-107

    • DOI

      10.4172/jpb.1000309

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dynamics of cell components during budding of Cryptococcus albidus yeast cells.2014

    • Author(s)
      Masashi Yamaguchi, Kiminori Shimizu, Susumu Kawamoto, Amalya A. Stepanova, Nataliya V. Vasilyeva
    • Journal Title

      Problems in Medical Mycology

      Volume: 16 Pages: 29-35

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Heterologous expression of a gene of Magnaporthe oryzae chrysovirus 1 strain A disrupts growth of the human pathogenic fungus Cryptococcus neoformans.2014

    • Author(s)
      Urayama SI, Fukuhara T, Moriyama H, Toh-E A, Kawamoto S.
    • Journal Title

      Microbiogy and Immunology

      Volume: 58 Issue: 5 Pages: 294-302

    • DOI

      10.1111/1348-0421.12148

    • NAID

      40020065609

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ordered kinetochore assembly in the human-pathogenic basidiomycetous yeast Cryptococcus neoformans2013

    • Author(s)
      Lukasz Kozubowski, Vikas Yadav Gautam Chatterjee, Shreyas Sridhar, Masashi Yamaguchi, Susumu Kawamoto, Indrani Bose,Joseph Heitman, Kaustuv Sanyal
    • Journal Title

      mBio

      Volume: 4 Issue: 5

    • DOI

      10.1128/mbio.00614-13

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A new F-actin structure in fungi: actin ring formation around the cell nucleus of Cryptococcus neoformans2013

    • Author(s)
      Marie Kopecka
    • Journal Title

      Microscopy

      Volume: 62 Issue: 2 Pages: 295-301

    • DOI

      10.1093/jmicro/dfs074

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] The CRZ1/SP1-like gene links survival under limited aeration, cell integrity and biofilm formation in the pathogenic yeast Cryptococcus neoformans2013

    • Author(s)
      Zuzana Moranova
    • Journal Title

      Biomedical Papers

      Volume: 157 Issue: 2 Pages: 212-220

    • DOI

      10.5507/bp.2013.024

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Towards understanding cell cycle regulation, hypoxic adaptation and nitric oxide (NO) signaling in Cryptococcus neoformans2015

    • Author(s)
      Susumu Kawamoto, Eric V Virtudazo, Zuzana Moranova, Misako Ohkusu, Kiminori Shimizu, Akio Toh-e, Hiroshi Takagi, Akiko Suganami, Yutaka Tamura, Vladislav Raclavsky
    • Organizer
      19th Congress of International Society for Human and Animal Mycology (ISHAM-2015)
    • Place of Presentation
      メルボルン会議センター(オーストラリア・メルボルン)
    • Year and Date
      2015-05-04 – 2015-05-08
    • Related Report
      2014 Annual Research Report
  • [Presentation] Towards understanding of cell cycle regulation and hypoxic signaling of Cryptococcus neoformans2014

    • Author(s)
      Susumu Kawamoto
    • Organizer
      講演会
    • Place of Presentation
      貴陽医学院(中国・貴州省・貴陽)
    • Year and Date
      2014-12-13
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Cryptococcus neoformansの細胞周期制御と低酸素ストレス応答の分子細胞シグナリング解析2014

    • Author(s)
      川本 進
    • Organizer
      第58回日本医真菌学会総会・学術集会学会賞記念講演
    • Place of Presentation
      ワークピア横浜(神奈川県・横浜市)
    • Year and Date
      2014-11-01 – 2014-11-02
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Cryptococcus neoformansの細胞周期制御と低酸素応答の分子機能解析2014

    • Author(s)
      川本 進
    • Organizer
      第87回日本生化学会大会シンポジウム「感染症研究における生化学的イノベーションの最前線」
    • Place of Presentation
      国立京都国際会館(京都府・京都市)
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 有用酵母研究から病原酵母研究へ2014

    • Author(s)
      川本 進
    • Organizer
      第21回酵母合同シンポジウム
    • Place of Presentation
      東京大学伊藤謝恩ホール(東京都・文京区)
    • Year and Date
      2014-09-03 – 2014-09-04
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Cell cycle regulation and hypoxic adaptation in pathogenic yeast, Cryptococcus neoformans2014

    • Author(s)
      Susumu Kawamoto, Eric V Virtudazo, Zuzana Moranova, Misako Ohkusu, Kiminori Shimizu, Akiko Suganami, Yutaka Tamura, Vladislav Raclavsky
    • Organizer
      International Union of Microbiological Societies Cpngress (IUMS-2014)
    • Place of Presentation
      モントリオール会議センター(カナダ・モントリオール)
    • Year and Date
      2014-07-27 – 2014-08-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] Towards understanding cell cycle control and hypoxic adaptation in Cryptococcus neoformans2014

    • Author(s)
      Susumu Kawamoto, Eric V Virtudazo, Zuzana Moranova, Misako Ohkusu, Kiminori Shimizu, Akiko Suganami, Yutaka Tamura, Vladislav Raclavsky
    • Organizer
      9th International Conference on Cryptococcus and Cryptococcosis (ICCC-9)
    • Place of Presentation
      王立熱帯研究所(オランダ・アムステルダム)
    • Year and Date
      2014-05-14 – 2014-05-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Towards understanding cell cycle control and hypoxic adaptation in Cryptococcus neoformans2014

    • Author(s)
      Susumu Kawamoto, Eric V Virtudazo, Zuzana Moranova, Misako Ohkusu, Kiminori Shimizu, Akiko Suganami, Yutaka Tamura, Vladislav Raclavsky
    • Organizer
      9th International Conference on Cryptococcus and Cryptococcosis (ICCC-9, 2014)
    • Place of Presentation
      Amsterdam, The Netherlands
    • Related Report
      2013 Research-status Report
  • [Presentation] Cell cycle regulation and hypoxic adaptation in pathogenic yeast, Cryptococcus neoformans2014

    • Author(s)
      Susumu Kawamoto, Eric V Virtudazo, Zuzana Moranova, Misako Ohkusu, Kiminori Shimizu, Akiko Suganami, Yutaka Tamura, Vladislav Raclavsky
    • Organizer
      International Union of Microbiological Societies Congress (IUMS-2104)
    • Place of Presentation
      Montreal, Canada
    • Related Report
      2013 Research-status Report
  • [Presentation] 病原酵母Cryptococcus neoformansの細胞周期制御と低酸素応答機構の解明に向けて2013

    • Author(s)
      川本 進
    • Organizer
      第86回日本生化学会大会シンポジウム「多様な生物に学ぶユニークな酵素・代謝機能とその応用」
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] カイコを用いた真菌感染症研究2013

    • Author(s)
      松本靖彦
    • Organizer
      第86回日本細菌学会総会ワークショップ「真菌症研究のニューフロンテイア」
    • Place of Presentation
      千葉
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Cell cycle regulation mechanism in pathogenic yeast, Cryptococcus neoformans: Structure-function relationship of G1 and G1/S cyclins homologue CnCln12013

    • Author(s)
      Susumu Kawamoto
    • Organizer
      2013 Annual Meeting of American Society for Biochemistry and Molecular Biology (Experimental Biology 2013)
    • Place of Presentation
      Boston, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] Towards understanding cell cycle control in Cryptococcus neoformans: Structure-function relationship of G1 and G1/S cyclins homologue CnCln12012

    • Author(s)
      Susumu Kawamoto
    • Organizer
      18th Congress of the International Society for Human and Animal Mycology (ISHAM 2012)
    • Place of Presentation
      Berlin, Germany
    • Related Report
      2012 Research-status Report
  • [Presentation] Study on cell cycle control mechanism in pathogenic yeast, Cryptococcus neoformans: Structure-function relationship of G1 and G1/S cyclins homologue CnCln12012

    • Author(s)
      Susumu Kawamoto
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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