Molecular and celluler signaling of hypoxic adaptation in the pathogenic yeast Cryptococcus neoformans
| Project/Area Number |
24590518
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Chiba University |
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Principal Investigator |
KAWAMOTO Susumu 千葉大学, 真菌医学研究センター, 教授 (80125921)
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| Co-Investigator(Renkei-kenkyūsha) |
HAGIWARA Daisuke 千葉大学, 真菌医学研究センター, 特任助教 (20612203)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 真菌 / クリプトコックス / 感染症 / 細胞周期 / 低酸素応答 / 環境適応 / G２-アレスト / 転写因子 / 低酸素ストレス / 病原真菌 / シグナル伝達 / 環境応答 / 細胞周期制御 / アグロバクテリウム / カルシニューリン |
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| Outline of Final Research Achievements |
Limited aeration has been demonstrated to cause slowdown in proliferation and delayed budding, resulting eventually in a unique unbudded G2-arrest in the obligate aerobic pathogenic yeast Cryptococcus neoformans. Also, the ability to adapt to decreased oxygen levels during pathogenesis has been identified as a virulence factor in C. neoformans. The aim of this study was to identify and characterize genes that are necessary for the proliferation slowdown and G2-arrest caused by limited aeration. This gene was identified as homologous both to calcineurin responsive (Crz1) and PKC1-dependent (Sp1-like) transcription factors. Our data demonstrate a tight molecular link between slowdown of proliferation during hypoxic adaptation and maintenance of cell integrity in C. neoformans and present a new role for the Crz1 family of transcription factors in fungi.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Dendritic cell-based immunization ameliorates pulmonary infection with highly virulent Cryptococcus gattii.2015
Author(s)
Keigo Ueno, Yuki Kinjo, Yoichiro Okubo, Kyoko Aki, Makoto Urai, Yukihiro Kaneko, Kiminori Shimizu, Dan-Ni Wang, Akiko Okawara, Takuya Nara, Kayo Ohkouchi, Yuki Mizuguchi, Susumu Kawamoto, Katsuhiko Kamei, Hideaki Ohno, Yoshihito Niki, Kazutoshi Shibuya, Yoshitsugu Miyazaki
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Journal Title
Infect. Immun.
Volume: 83
Issue: 4
Pages: 1577-1586
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Towards understanding cell cycle regulation, hypoxic adaptation and nitric oxide (NO) signaling in Cryptococcus neoformans2015
Author(s)
Susumu Kawamoto, Eric V Virtudazo, Zuzana Moranova, Misako Ohkusu, Kiminori Shimizu, Akio Toh-e, Hiroshi Takagi, Akiko Suganami, Yutaka Tamura, Vladislav Raclavsky
Organizer
19th Congress of International Society for Human and Animal Mycology (ISHAM-2015)
Place of Presentation
メルボルン会議センター（オーストラリア・メルボルン）
Year and Date
2015-05-04 – 2015-05-08
Related Report
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[Presentation] 有用酵母研究から病原酵母研究へ2014
Author(s)
川本 進
Organizer
第21回酵母合同シンポジウム
Place of Presentation
東京大学伊藤謝恩ホール（東京都・文京区）
Year and Date
2014-09-03 – 2014-09-04
Related Report
Invited
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