Cell cycle-related protein kinase in Aspergillus fumigatus

• Project/Area Number: 24590546
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: UMEYAMA Takashi 国立感染症研究所, その他部局等, 研究員 (20360696)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: Aspergillus fumigatus / アスペルギルス症 / シグナル伝達 / プロテインキナーゼ / 病原真菌 / 真菌症 / 糸状菌 / 細胞周期

Outline of Final Research Achievements

Overall mortality with invasive pulmonary aspergillosis was over 50%, leading to strong expectation for development of a novel therapeutics. Aim of study is to elucidate correlation between cell cycle regulation and filamentous growth via analysis of protein kinases in Aspergillus fumigatus, the major causative agent of aspergillosis. I analyzed 8 protein kinases by inhibitors, gene disruption, gene downregulation, and chemical genetics. I found that Mps1 are essential for the growth. These results could contribute to basic research for a novel therapeutics of aspergillosis and give a new insight of unique mechanism for cell cycle regulation into basic biology field.

Research Products

• [Journal Article] Invasive pulmonary aspergillosis due to Aspergillus lentulus: Successful treatment of a liver transplant patient. (2015)
  • Author(s): Yoshida H, Seki M, Umeyama T, Urai M, Kinjo Y, Nishi I, Toyokawa M, Kaneko Y, Ohno H, Miyazaki Y, Tomono K.
  • Journal Title: J Infect Chemother Volume: epub Issue: 6 Pages: 479-481
  • DOI: 10.1016/j.jiac.2015.02.010
  • Peer Reviewed / Open Access
• [Journal Article] Aspergillus fungal ball in central cavity of a pulmonary cryptococcal nodal lesion. (2015)
  • Author(s): Kimura M, Maenishi O, Enoki E, Chikugo T, Umeyama T, Ohno H, Miyazaki Y.
  • Journal Title: Pathol Int. Volume: epub Issue: 6 Pages: 329-331
  • DOI: 10.1111/pin.12266
  • Peer Reviewed
• [Journal Article] Potent drugs that attenuate anti-Candida albicans activity of fluconazole and their possible mechanisms of action. (2014)
  • Author(s): Urai M, Kaneko Y, Niki M, Inoue M, Tanabe K, Umeyama T, Fukazawa H, Ohno H, Miyazaki Y.
  • Journal Title: J Infect Chemother Volume: 20 Issue: 10 Pages: 612-615
  • DOI: 10.1016/j.jiac.2014.06.004
  • Peer Reviewed / Open Access
• [Journal Article] Histopathological study of murine pulmonary cryptococcosis induced by Cryptococcus gattii and Cryptococcus neoformans. (2013)
  • Author(s): Yoichiro Okubo, Megumi Wakayama, Hideaki Ohno, Shuhei Yamamoto, Naobumi Tochigi, Koichi Tanabe, Yukihiro Kaneko, Satoshi Yamagoe, Takashi Umeyama, et al.
  • Journal Title: Jpn J Infect Dis, Volume: 66 Pages: 216-221
  • NAID: 130003381674
  • Peer Reviewed
• [Journal Article] Application of nested PCR for diagnosis of histoplasmosis. (2013)
  • Author(s): Hideaki Ohno, Koichi Tanabe, Takashi Umeyama, Yukihiro Kaneko, Satoshi Yamagoe, and Yoshitsugu Miyazaki.
  • Journal Title: J Infect Chemother Volume: 19 Issue: 5 Pages: 999-1003
  • DOI: 10.1007/s10156-013-0548-2
  • Peer Reviewed
• [Journal Article] The Candida glabrata sterol scavenging mechanism, mediated by the ATP-binding cassette transporter Aus1p, is regulated by iron limitation. (2013)
  • Author(s): Nagi M, Tanabe K, Ueno K, Nakayama H, Aoyama T, Chibana H, Yamagoe S, Umeyama T, Oura T, Ohno H, Kajiwara S, Miyazaki Y.
  • Journal Title: Mol Microbiol. Volume: 88 Issue: 2 Pages: 371-81
  • DOI: 10.1111/mmi.12189
  • Peer Reviewed
• [Journal Article] Determination of Epidemiology of Clinically Isolated <i>Cryptococcus neoformans</i> Strains in Japan by Multilocus Sequence Typing (2013)
  • Author(s): Umeyama T, Ohno H, Minamoto F, Takagi T, Tanamachi C, Tanabe K, Kaneko Y, Yamagoe S, Kishi K, Fujii T, Takemura H, Watanabe H, Miyazaki Y.
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 66 Issue: 1 Pages: 51-55
  • DOI: 10.7883/yoken.66.51
  • NAID: 130003381641
  • ISSN: 1344-6304, 1884-2836
  • Peer Reviewed
• [Journal Article] Serum cholesterol promotes the growth of Candida glabrata in the presence of fluconazole (2013)
  • Author(s): Nagi M, Tanabe K, Nakayama H, Yamagoe S, Umeyama T, Oura T, Ohno H, Kajiwara S, Miyazaki Y
  • Journal Title: Journal of Infection and Chemotherapy Volume: 19 Issue: 1 Pages: 138-143
  • DOI: 10.1007/s10156-012-0531-3
  • Peer Reviewed
• [Journal Article] The mannan antigen lacking β-1,2-linked oligomannosides from Candida albicans increases production of inflammatory cytokines released by dendritic cells (2013)
  • Author(s): Ueno, K.
  • Journal Title: Med. Microbiol. Volume: 51 Issue: 4 Pages: 385-395
  • DOI: 10.3109/13693786.2012.733892
  • Peer Reviewed
• [Journal Article] A First Report of <i>Rothia aeria</i> Endocarditis Complicated by Cerebral Hemorrhage (2012)
  • Author(s): Tarumoto N, Sujino K, Yamaguchi T, Umeyama T, Ohno H, Miyazaki Y, Maesaki S.
  • Journal Title: Internal Medicine Volume: 51 Issue: 23 Pages: 3295-3299
  • DOI: 10.2169/internalmedicine.51.7946
  • NAID: 130003308145
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed
• [Journal Article] Multilocus sequence typing of Cryptococcus neoformans in non-HIV associated cryptococcosis in Nagasaki, Japan (2012)
  • Author(s): Mihara T, Izumikawa K, Kakeya H, Kohno S, et al.
  • Journal Title: Med Mycol Volume: 51 Issue: 3 Pages: 252-260
  • DOI: 10.3109/13693786.2012.708883
  • Peer Reviewed
• [Presentation] アスペルギルスの抗真菌薬耐性 (2014)
  • Author(s): 梅山 隆、山越 智、田辺公一、名木稔、大野秀明、宮﨑義継
  • Organizer: 第58回日本医真菌学会総会・学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-01 – 2014-11-02
• [Presentation] 病原糸状菌Aspergillus fumigatusのPolo-likeキナーゼ遺伝子破壊株の菌糸成長・分生子形成・抗真菌薬感受性への影響 (2014)
  • Author(s): 梅山 隆、山越 智、田辺公一、名木稔、金子幸弘、金城雄樹、大野秀明、宮﨑義継
  • Organizer: 第88回日本感染症学会学術講演会・第62回日本化学療法学会総会合同学会
  • Place of Presentation: 福岡
  • Year and Date: 2014-06-18 – 2014-06-20
• [Presentation] 症例から学ぶ感染症セミナーームーコル症の真菌同定検査 (2014)
  • Author(s): 梅山 隆、大野秀明、田辺公一、山越 智、名木稔、宮﨑義継
  • Organizer: 第88回日本感染症学会学術講演会・第62回日本化学療法学会総会合同学会
  • Place of Presentation: 福岡
  • Year and Date: 2014-06-18 – 2014-06-20
• [Presentation] Polo-like kinase regulates colony growth, conidiation, and cell polarity in Aspergillus fumigatus. (2014)
  • Author(s): Takashi Umeyama, Satoshi Yamagoe, Koichi Tanabe, Minoru Nagi, Hideaki Ohno, and Yoshitsugu Miyazaki.
  • Organizer: 6th Advances in Aspergillus and Aspergillosis
  • Place of Presentation: Madrid, Spain
• [Presentation] 国立感染症研究所の真菌同定検査の取り組みと培養陰性で遺伝子検査で同定できた4症例. (2014)
  • Author(s): 梅山 隆、田辺公一、金子幸弘、壇辻百合香、中山靖子、荒岡秀樹、高野淳一朗、中島由紀子、大川原明子、山越 智、名木 稔、上野圭吾、金城雄樹、大野秀明、宮﨑義継.
  • Organizer: 第25回日本臨床微生物学会総会
  • Place of Presentation: 名古屋
• [Presentation] Mps1 kinase is required for normal nuclear segregation in Aspergillus fumigatus. (2014)
  • Author(s): Umeyama T, Yamagoe S, Tanabe K, Nagi M, Kaneko Y, Kinjo Y, Ohno H, Miyazaki Y
  • Organizer: 2013 Congress of Asia Pacific Society for Medical Mycology.
  • Place of Presentation: Chengdu, China
• [Presentation] Aspergillus fumigatusのPolo-likeキナーゼは菌糸伸長と分生子形成を制御している. (2013)
  • Author(s): 梅山 隆, 山越 智, 田辺公一, 名木 稔, 金子幸弘, 金城雄樹, 大野秀明, 宮﨑義継
  • Organizer: 第57回日本医真菌学会総会・学術集会
  • Place of Presentation: 東京
• [Presentation] Aspergillus fumigatusの肺胞上皮細胞への接着と侵入機構 (2012)
  • Author(s): 梅山 隆, 山越 智, 田辺公一, 大野秀明, 宮﨑義継
  • Organizer: 第33回関東医真菌懇話会学術集会
  • Place of Presentation: 東京
  • Invited
• [Presentation] Aspergillus fumigatusプロテインキナーゼの特異的阻害による病原性制御 (2012)
  • Author(s): 梅山 隆, 山越 智, 田辺公一, 大野秀明, 宮﨑義継
  • Organizer: 第60回日本化学療法学会学術集会
  • Place of Presentation: 長崎
• [Presentation] 侵襲性肺アスペルギルス症の主要原因菌Aspergillus fumigatusによる肺胞上皮細胞への接着と侵入. (2012)
  • Author(s): 梅山 隆, 山越 智, 田辺公一, 大野秀明, 宮﨑義継
  • Organizer: 第86回日本感染症学会総会・学術講演会
  • Place of Presentation: 長崎
• [Remarks] 国立感染症研究所 真菌部
  • URL: http://www.nih.go.jp/niid/ja/from-bioact.html